FastQCFastQC Report
Fri 10 Feb 2017
ERR1631056.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631056.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203970
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT152827.492278276217091No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG145267.121635534637447No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT111045.443937833995196TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC75993.725547874687454No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC46232.266509780850125RNA PCR Primer, Index 12 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC36031.7664362406236211No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24161.1844879148894445No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22811.1183017110359368No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG17890.8770897681031524No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14170.6947100063734863No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA13750.6741187429523949No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT11390.5584154532529293No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT9390.4603618179143992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9230.4525175270873168No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8730.4280041182526842No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8270.40545178212482225No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7350.3603471098690984No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT6030.29563171054566845No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6030.29563171054566845No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5950.29170956513212726No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG5580.27356964259449923No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5460.26768642447418733No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5210.2554297200568711No Hit
ATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCT3980.19512673432367506TruSeq Adapter, Index 12 (95% over 21bp)
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3980.19512673432367506No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3930.19267539344021178No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3770.18483110261312938No Hit
TCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT3740.18336029808305143TruSeq Adapter, Index 12 (95% over 24bp)
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3690.18090895719958816No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3550.17404520272589105No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG3550.17404520272589105No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3520.1725743981958131No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3500.1715938618424278No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3470.17012305731234986No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG3380.165710643722116No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG3330.16325930283865275No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3310.16227876648526743No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3260.15982742560180419No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3250.15933715742511154No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3100.15198313477472178No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3020.14806098936118056No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG2950.144629112124332No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG2920.14315830759425408No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2890.1416875030641761No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2880.14119723488748345No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG2870.1407069667107908No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC2760.13531401676717164No Hit
CTTTACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA2710.1328626758837084No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG2690.1318821395303231No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT2690.1318821395303231No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC2580.1264891895867039No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2580.1264891895867039No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT2420.11864489875962152No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2410.11815463058292887No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2360.11570328969946561No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2340.11472275334608031No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA2330.11423248516938765No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC2190.10736873069569054No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG2170.10638819434230523No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2120.10393685345884199No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2080.10197578075207138No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG2070.10148551257537874No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2060.1009952443986861No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGC200.001838388937.03
ACAGACA501.7826096E-1037.017
ACGCATC308.278477E-637.01
GACAGAC501.7826096E-1037.016
ATTACGG200.001838388937.02
TCAGAGT308.278477E-637.03
GATTACG200.001838388937.01
CGCATCT308.278477E-637.02
TGTGCAA200.001838388937.010
TTACGGG200.001838388937.03
CTTATAC17650.035.8470231
TTATACA18750.033.7440032
CAGACAC554.947651E-1033.63636418
AGACAGA554.947651E-1033.63636415
CGTCTTC5800.032.85344737
ACTCAGA352.3787577E-531.7142851
TATACAC20400.031.1053923
GAGACAG601.2569217E-930.83333414
ACACGAA250.005485879329.617
GATATGT250.005485879329.61
Produced by FastQC (version 0.11.5)