##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631056.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	203970
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.194533509829878	31.0	31.0	34.0	28.0	34.0
2	31.263867235377752	31.0	31.0	34.0	28.0	34.0
3	30.686659802912192	31.0	31.0	34.0	26.0	34.0
4	34.81053096043536	37.0	35.0	37.0	32.0	37.0
5	35.062151296759325	35.0	35.0	37.0	32.0	37.0
6	35.28275236554395	37.0	35.0	37.0	32.0	37.0
7	35.26635779771535	37.0	35.0	37.0	32.0	37.0
8	35.30471147717802	37.0	35.0	37.0	32.0	37.0
9	37.136284747757024	39.0	37.0	39.0	34.0	39.0
10	36.94931607589351	39.0	37.0	39.0	33.0	39.0
11	36.97572191989018	39.0	37.0	39.0	33.0	39.0
12	36.870829043486786	39.0	37.0	39.0	33.0	39.0
13	36.99220963867236	39.0	37.0	39.0	33.0	39.0
14	37.80805020346129	39.0	37.0	41.0	33.0	41.0
15	37.868117860469674	39.0	37.0	41.0	33.0	41.0
16	37.805142913173505	39.0	37.0	41.0	33.0	41.0
17	37.80515271853704	39.0	37.0	41.0	33.0	41.0
18	37.97071137912438	40.0	37.0	41.0	33.0	41.0
19	37.90057361376673	40.0	37.0	41.0	33.0	41.0
20	37.959092023336765	40.0	37.0	41.0	33.0	41.0
21	37.7686179340099	39.0	37.0	41.0	32.0	41.0
22	37.41268323773104	39.0	36.0	41.0	31.0	41.0
23	37.54942393489239	39.0	36.0	41.0	32.0	41.0
24	37.28290925136049	39.0	36.0	41.0	31.0	41.0
25	37.20226503897632	39.0	36.0	40.0	31.0	41.0
26	37.10716281806148	39.0	36.0	40.0	31.0	41.0
27	36.79981860077462	39.0	35.0	40.0	30.0	41.0
28	36.89548953277443	39.0	35.0	40.0	31.0	41.0
29	36.522307202039514	39.0	35.0	40.0	30.0	41.0
30	36.33688287493259	38.0	35.0	40.0	30.0	41.0
31	36.2999852919547	38.0	35.0	40.0	30.0	41.0
32	35.699421483551504	38.0	34.0	40.0	27.0	41.0
33	35.69848016865225	38.0	34.0	40.0	27.0	41.0
34	35.721508064911504	38.0	34.0	40.0	28.0	41.0
35	35.594616855419915	38.0	34.0	40.0	27.0	41.0
36	35.605211550718245	38.0	34.0	40.0	28.0	41.0
37	35.66927489336667	38.0	34.0	40.0	28.0	41.0
38	35.58064911506594	38.0	34.0	40.0	27.0	41.0
39	35.22279256753444	38.0	34.0	40.0	26.0	41.0
40	34.87053488258077	38.0	33.0	40.0	26.0	41.0
41	34.84206010687846	38.0	33.0	40.0	25.0	41.0
42	34.676192577339805	38.0	33.0	40.0	24.0	41.0
43	33.497730058341915	36.0	31.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	14.0
10	20.0
11	13.0
12	14.0
13	20.0
14	14.0
15	11.0
16	6.0
17	9.0
18	12.0
19	31.0
20	66.0
21	96.0
22	177.0
23	409.0
24	714.0
25	1059.0
26	1648.0
27	2429.0
28	3231.0
29	4286.0
30	5381.0
31	6811.0
32	8390.0
33	10472.0
34	13067.0
35	17495.0
36	22710.0
37	31827.0
38	43718.0
39	29818.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.02549394518802	24.684022160121586	17.547188312006668	27.74329558268373
2	11.01534539393048	19.736725989116046	44.83845663577977	24.409471981173702
3	12.732754816884837	31.673775555228712	37.42216992695005	18.171299700936412
4	8.61646320537334	21.844879148894446	26.169044467323626	43.36961317840859
5	12.791586998087952	37.45207628572829	30.86385252733245	18.892484188851302
6	24.586458792959746	36.91915477766338	15.312545962641567	23.181840466735302
7	20.20150022062068	35.13163700544198	14.469774966906899	30.19708780703045
8	26.56567142226798	28.572339069471003	25.614060891307545	19.247928616953473
9	18.741971858606657	10.981026621561995	13.40834436436731	56.86865715546404
10	14.887483453449038	26.691180075501297	27.40991322253273	31.01142324851694
11	28.4723243614257	18.71794871794872	23.048487522674904	29.76123939795068
12	17.62317987939403	26.88924841888513	19.439133205863605	36.04843849585723
13	36.77060352012551	14.625189978918469	23.977055449330784	24.62715105162524
14	22.67784478109526	26.21611021228612	19.808305142913174	31.29773986370545
15	29.809285679266555	23.891748786586263	16.50242682747463	29.79653870667255
16	30.972201794381526	20.8339461685542	16.095994508996423	32.097857528067856
17	17.561406089130756	36.18326224444771	16.429376869147426	29.825954797274107
18	30.29612197872236	14.931607589351376	18.937588861107024	35.834681570819235
19	29.62102269941658	25.235573858900818	18.878266411727214	26.265137029955387
20	22.009609256263175	18.478697847722707	19.29891650732951	40.21277638868461
21	37.43246555866059	16.838260528509092	21.478648820905036	24.250625091925283
22	17.41285483159288	38.53164681080551	25.093396087659954	18.962102269941656
23	30.546158748835612	21.795362063048486	19.91077119184194	27.747707996273963
24	35.312055694464874	23.7824189831838	23.791734078540962	17.113791243810365
25	18.044810511349706	29.919596019022404	25.649360200029413	26.38623326959847
26	38.85375300289258	25.436093543168113	20.16473010736873	15.545423346570574
27	17.994312889150365	30.511839976467126	28.12472422415061	23.369122910231894
28	24.54037358435064	22.860714811001618	25.795950384860518	26.802961219787225
29	29.734274648232585	18.124724224150608	29.12291023189685	23.018090895719958
30	16.648036475952345	25.50718242878855	32.23856449477864	25.606216600480465
31	37.17997744766387	19.88527724665392	19.850468206108744	23.084277099573466
32	20.866303868215912	20.83786831396774	41.169289601411975	17.126538216404374
33	16.63381869882826	34.397215276756384	31.043290680001963	17.925675344413396
34	24.524685002696476	30.10050497622199	23.953522576849537	21.421287444231996
35	17.912928371819387	18.831690934941413	46.70147570721184	16.553904986027355
36	22.670000490268176	27.15742511153601	20.713340197087806	29.45923420110801
37	21.02613129381772	25.075256165122322	29.45923420110801	24.439378339951954
38	20.17747707996274	16.48085502770015	31.25018385056626	32.091484041770855
39	29.943619159680345	18.635583664264352	24.305044859538167	27.115752316517135
40	17.204000588321815	17.058881208020786	43.99862724910526	21.73849095455214
41	24.044712457714372	21.937539834289357	22.504780114722752	31.512967593273522
42	24.350149531793893	16.553904986027355	37.46041084473207	21.635534637446682
43	25.149776927979605	16.80100014708045	33.36961317840859	24.67960974653135
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	37.0
4	72.0
5	72.0
6	111.0
7	150.0
8	166.5
9	183.0
10	289.0
11	395.0
12	395.0
13	626.5
14	858.0
15	2217.0
16	3576.0
17	3608.0
18	3640.0
19	3640.0
20	2963.0
21	2286.0
22	1775.5
23	1265.0
24	1200.0
25	1135.0
26	1135.0
27	1104.5
28	1074.0
29	1093.5
30	1113.0
31	1148.5
32	1184.0
33	1184.0
34	1499.0
35	1814.0
36	1788.5
37	1763.0
38	2007.0
39	2251.0
40	2251.0
41	2934.5
42	3618.0
43	4837.5
44	6057.0
45	8278.0
46	10499.0
47	10499.0
48	14989.5
49	19480.0
50	33114.0
51	46748.0
52	32945.5
53	19143.0
54	19143.0
55	27593.0
56	36043.0
57	29359.5
58	22676.0
59	14849.5
60	7023.0
61	7023.0
62	5715.5
63	4408.0
64	3452.0
65	2496.0
66	2070.5
67	1645.0
68	1645.0
69	1270.0
70	895.0
71	613.5
72	332.0
73	206.0
74	80.0
75	80.0
76	54.0
77	28.0
78	22.5
79	17.0
80	15.0
81	13.0
82	13.0
83	9.0
84	5.0
85	3.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	203970.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.499289111143796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.66141043648796	13.25685149776928
2	10.860519969257679	4.0182379761729665
3	4.661701958497867	2.5871451684071185
4	2.530941086051997	1.8728244349659264
5	1.5371160522619458	1.4217777124086874
6	1.2429438422600907	1.3796146492131196
7	0.8719158296451381	1.1290876109231751
8	0.6519492221662736	0.9648477717311369
9	0.5538918188323219	0.9221944403588762
>10	4.433784750748682	16.36221012894053
>50	0.6121962208146715	7.910967299112615
>100	0.32067421090292314	11.464431043780948
>500	0.029152200991174836	3.7863411285973427
>1k	0.021201600720854422	9.140069618081089
>5k	0.002650200090106803	3.725547874687454
>10k+	0.007950600270320409	20.057851644849734
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT	15282	7.492278276217091	No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG	14526	7.121635534637447	No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	11104	5.443937833995196	TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC	7599	3.725547874687454	No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC	4623	2.266509780850125	RNA PCR Primer, Index 12 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC	3603	1.7664362406236211	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2416	1.1844879148894445	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2281	1.1183017110359368	No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG	1789	0.8770897681031524	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1417	0.6947100063734863	No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA	1375	0.6741187429523949	No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT	1139	0.5584154532529293	No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT	939	0.4603618179143992	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	923	0.4525175270873168	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	873	0.4280041182526842	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	827	0.40545178212482225	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	735	0.3603471098690984	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	603	0.29563171054566845	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	603	0.29563171054566845	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	595	0.29170956513212726	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	558	0.27356964259449923	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	546	0.26768642447418733	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	521	0.2554297200568711	No Hit
ATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCT	398	0.19512673432367506	TruSeq Adapter, Index 12 (95% over 21bp)
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	398	0.19512673432367506	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	393	0.19267539344021178	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	377	0.18483110261312938	No Hit
TCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT	374	0.18336029808305143	TruSeq Adapter, Index 12 (95% over 24bp)
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	369	0.18090895719958816	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	355	0.17404520272589105	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	355	0.17404520272589105	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	352	0.1725743981958131	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	350	0.1715938618424278	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	347	0.17012305731234986	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	338	0.165710643722116	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	333	0.16325930283865275	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	331	0.16227876648526743	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	326	0.15982742560180419	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	325	0.15933715742511154	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	310	0.15198313477472178	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	302	0.14806098936118056	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	295	0.144629112124332	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	292	0.14315830759425408	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	289	0.1416875030641761	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	288	0.14119723488748345	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	287	0.1407069667107908	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	276	0.13531401676717164	No Hit
CTTTACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA	271	0.1328626758837084	No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG	269	0.1318821395303231	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	269	0.1318821395303231	No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	258	0.1264891895867039	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	258	0.1264891895867039	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	242	0.11864489875962152	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	241	0.11815463058292887	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	236	0.11570328969946561	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	234	0.11472275334608031	No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA	233	0.11423248516938765	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	219	0.10736873069569054	No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG	217	0.10638819434230523	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	212	0.10393685345884199	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	208	0.10197578075207138	No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG	207	0.10148551257537874	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	206	0.1009952443986861	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	9.805363533853017E-4	0.0
11	0.0	0.0	0.0	9.805363533853017E-4	0.0
12	0.0	0.0	0.0	9.805363533853017E-4	0.0
13	0.0	0.0	0.0	0.0014708045300779527	0.0
14	0.0	0.0	0.0	0.0014708045300779527	0.0
15	0.0	0.0	0.0	0.0014708045300779527	0.0
16	0.0	0.0	0.0	0.0019610727067706033	0.0
17	0.0	0.0	0.0	0.0024513408834632546	0.0
18	0.0	0.0	0.0	0.0024513408834632546	0.0
19	0.0	0.0	0.0	0.0024513408834632546	0.0
20	0.0	0.0	0.0	0.0029416090601559054	0.0
21	0.0	0.0	0.0	0.004412413590233858	0.0
22	0.0	0.0	0.0	0.004902681766926509	0.0
23	0.0	0.0	0.0	0.0068637544736971125	0.0
24	0.0	0.0	0.0	0.007844290827082413	0.0
25	0.0	0.0	0.0	0.007844290827082413	0.0
26	0.0	0.0	0.0	0.008824827180467716	0.0
27	0.0	0.0	0.0	0.009315095357160366	0.0
28	0.0	0.0	0.0	0.02745501789478845	0.0
29	0.0	0.0	0.0	0.05245869490611364	0.0
30	0.0	0.0	0.0	0.0848163945678286	0.0
31	0.0	0.0	0.0	0.1387458940040202	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACGC	20	0.0018383889	37.0	3
ACAGACA	50	1.7826096E-10	37.0	17
ACGCATC	30	8.278477E-6	37.0	1
GACAGAC	50	1.7826096E-10	37.0	16
ATTACGG	20	0.0018383889	37.0	2
TCAGAGT	30	8.278477E-6	37.0	3
GATTACG	20	0.0018383889	37.0	1
CGCATCT	30	8.278477E-6	37.0	2
TGTGCAA	20	0.0018383889	37.0	10
TTACGGG	20	0.0018383889	37.0	3
CTTATAC	1765	0.0	35.847023	1
TTATACA	1875	0.0	33.744003	2
CAGACAC	55	4.947651E-10	33.636364	18
AGACAGA	55	4.947651E-10	33.636364	15
CGTCTTC	580	0.0	32.853447	37
ACTCAGA	35	2.3787577E-5	31.714285	1
TATACAC	2040	0.0	31.105392	3
GAGACAG	60	1.2569217E-9	30.833334	14
ACACGAA	25	0.0054858793	29.6	17
GATATGT	25	0.0054858793	29.6	1
>>END_MODULE