FastQCFastQC Report
Fri 10 Feb 2017
ERR1631055.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631055.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452674
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT226375.000729001444748No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG156743.4625359530257978No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT155333.4313877094774603RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC78081.7248616001802621No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC67581.4929065950330702RNA PCR Primer, Index 17 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT52441.1584495685636904No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50841.1231040439698325No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC47941.0590402806434651No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA31020.6852613580634187No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20440.4515390766865338No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA18280.4038226184848257No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA18240.40293898036997927No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT16980.37510437975231625No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG16550.365605270017717No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA14610.3227488214476643No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTAT14140.31236607359821855No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT13390.2957978589448477No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC12990.28696147779638326No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT12790.28254328722215105No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG12200.26950962502816594No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG11160.24653503404215835No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC11020.2434423006401958No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT9830.21715406672351403No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG9730.21494497143639793No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8720.1926331090365252No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG8570.18931946610585101No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT8260.18247127071579106No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG8200.18114581354352138No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG7700.1701003371079408No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG7640.16877487993567114No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG7360.16258941313174602No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG7350.16236850360303443No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG6910.1526484843397235No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG6770.14955575093776097No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6580.14535846989224033No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6470.1429284650764126No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6340.14005664120316166No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG6260.13828936497346875No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6110.1349757220427946No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5850.1292320742962927No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA5710.12613934089433013No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC5700.12591843136561853No Hit
ATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT5390.11907023597555856No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT5230.1155356835161728No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5150.1137684072864799No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5120.11310567870034506No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4820.10647839283899672No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG4800.1060365737815735No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4650.10272293085089933No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC4590.10139747367862967No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAAGCA200.001840757937.036
AGCACGG200.001840757937.02
CTTATAC27400.035.2445261
GATTACG800.034.68751
TTATACA28600.033.701052
TATACAC30150.032.152573
CGTCTTC7100.031.78873437
TGATACA303.5960617E-430.8333342
TCAGAGT551.850458E-830.2727283
TAGGACC801.8189894E-1230.06254
TTGTATC250.00549286829.62
GTTGTAT250.00549286829.61
CAAGCAT250.00549286829.637
GAGCACG250.00549286829.61
TCAAGAC250.00549286829.62
TGCGTCG250.00549286829.610
ATTACTT454.0012037E-628.7777772
AGGACCG604.310641E-827.7500025
CTTAGGA604.310641E-827.7500022
GATACAC405.9322992E-527.753
Produced by FastQC (version 0.11.5)