##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631055.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	452674
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.351690178804173	31.0	31.0	34.0	28.0	34.0
2	31.363727539023667	31.0	31.0	34.0	28.0	34.0
3	30.800987023774283	31.0	31.0	34.0	26.0	34.0
4	34.93984633533183	37.0	35.0	37.0	32.0	37.0
5	35.18702200700725	37.0	35.0	37.0	32.0	37.0
6	35.40532480328007	37.0	35.0	37.0	33.0	37.0
7	35.40703022484172	37.0	35.0	37.0	33.0	37.0
8	35.425752307400025	37.0	35.0	37.0	33.0	37.0
9	37.23018331072692	39.0	37.0	39.0	34.0	39.0
10	37.05993054604417	39.0	37.0	39.0	33.0	39.0
11	37.13484317632557	39.0	37.0	39.0	33.0	39.0
12	37.04108917234036	39.0	37.0	39.0	33.0	39.0
13	37.13860526559952	39.0	37.0	39.0	33.0	39.0
14	38.09592333555715	40.0	37.0	41.0	33.0	41.0
15	38.1216173228416	40.0	37.0	41.0	33.0	41.0
16	38.12318798959074	40.0	37.0	41.0	33.0	41.0
17	38.098070576176234	40.0	37.0	41.0	33.0	41.0
18	38.21111660930383	40.0	38.0	41.0	33.0	41.0
19	38.146564635919006	40.0	37.0	41.0	33.0	41.0
20	38.15349677692998	40.0	37.0	41.0	33.0	41.0
21	38.09074300710887	40.0	37.0	41.0	33.0	41.0
22	37.97072065106456	40.0	37.0	41.0	33.0	41.0
23	38.01492243866447	40.0	37.0	41.0	33.0	41.0
24	37.887813304939094	40.0	37.0	41.0	33.0	41.0
25	37.82161997375595	40.0	37.0	41.0	33.0	41.0
26	37.639594940288156	39.0	37.0	41.0	33.0	41.0
27	37.52149891533421	39.0	36.0	41.0	32.0	41.0
28	37.52801132824063	39.0	36.0	41.0	32.0	41.0
29	37.418248010709696	39.0	36.0	41.0	32.0	41.0
30	37.214419206758066	39.0	36.0	40.0	31.0	41.0
31	37.12151349536311	39.0	36.0	40.0	31.0	41.0
32	36.934213142349684	39.0	35.0	40.0	31.0	41.0
33	36.82851897833761	39.0	35.0	40.0	31.0	41.0
34	36.787988707104894	39.0	35.0	40.0	31.0	41.0
35	36.68146613236015	39.0	35.0	40.0	30.0	41.0
36	36.58019457711289	39.0	35.0	40.0	30.0	41.0
37	36.50729664173335	39.0	35.0	40.0	30.0	41.0
38	36.42796361178243	39.0	35.0	40.0	30.0	41.0
39	36.20406738624264	39.0	35.0	40.0	30.0	41.0
40	35.95697786928341	38.0	35.0	40.0	28.0	41.0
41	35.879025523886945	38.0	35.0	40.0	28.0	41.0
42	35.721735730348996	38.0	35.0	40.0	28.0	41.0
43	34.840366356362416	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	8.0
10	11.0
11	11.0
12	8.0
13	8.0
14	8.0
15	4.0
16	7.0
17	9.0
18	14.0
19	35.0
20	89.0
21	165.0
22	289.0
23	542.0
24	924.0
25	1593.0
26	2424.0
27	3726.0
28	5327.0
29	7299.0
30	9485.0
31	12294.0
32	15297.0
33	19779.0
34	25717.0
35	34511.0
36	46977.0
37	68716.0
38	102970.0
39	94424.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.45559497563368	21.31997861595762	16.481176299058482	25.743250109350218
2	13.08645957134715	21.37211326473356	42.6505608892934	22.89086627462589
3	14.183275381400302	30.38941931721283	35.10053592651665	20.326769374870217
4	10.106169119498801	20.419993195986518	32.995268117895	36.47856956661968
5	13.321507309896305	36.95816415345259	32.74696580762315	16.973362729027954
6	26.069754392785978	39.53176016294287	15.87234963792928	18.52613580634187
7	23.541223927152874	32.35926958473427	19.32649102886404	24.77301545924882
8	26.504062526233007	31.357886691084534	23.012587424945988	19.12546335773647
9	22.04544550824655	12.415557332649986	17.05775016899579	48.48124699010767
10	15.679495619364046	27.57348555472592	29.341203603476234	27.405815222433805
11	30.175137074362567	21.517913553683226	24.201964327529303	24.104985044424907
12	20.048423368693584	26.78395489911062	23.680838749298612	29.486782982897186
13	32.64623106253065	17.701259626132714	26.03264159196243	23.619867719374206
14	22.967521881088818	23.626274095706844	24.398131989025217	29.008072034179122
15	26.995144408558918	25.91114135117104	20.66586550144254	26.427848738827503
16	28.125759376504945	23.258901549459434	20.58125715194599	28.034081922089626
17	19.776483738849592	32.19977290500449	21.331024092393204	26.692719263752725
18	26.136469070456886	21.091779072798523	23.755285260474427	29.016466596270163
19	25.52278239969603	27.62098110339891	23.87678550126581	22.979450995639244
20	26.4430914963086	20.787586651762638	24.312198182356397	28.457123669572365
21	29.295033511975504	21.62129921312026	23.973322965312786	25.110344309591447
22	20.59627899989838	32.818319585397	23.973985693898918	22.611415720805702
23	26.035955234893105	24.148725131109806	24.750703596848947	25.064616037148145
24	27.867957956498497	27.70647309101031	23.858450010382747	20.567118942108447
25	21.779249526149062	32.8145641234089	24.301815434506953	21.10437091593509
26	27.510305429514396	30.189054374671397	23.66139871077199	18.639241485042216
27	23.113543079567194	31.034254231522024	25.71497368967513	20.137228999235653
28	21.72203395821275	25.033909612657233	28.36169075316895	24.882365675961065
29	22.438222650295796	27.601541064872293	24.304687258380202	25.65554902645171
30	24.252994428661687	27.135421959290788	28.72773784224409	19.883845769803436
31	31.931146918091162	21.665922937920005	23.135854941967064	23.267075202021765
32	21.097301811016315	26.360471332570455	27.17187203152821	25.370354824885016
33	18.876056499821065	31.45773779806218	29.704378868678123	19.961826833438636
34	23.717288821536027	28.142106681629603	26.110180836540202	22.03042366029416
35	19.833036578199764	28.1438739578593	33.70173679071473	18.321352673226208
36	23.07731391685849	28.15602398193844	23.703371521227197	25.063290579975877
37	21.33765137825455	26.158339113799332	28.361469843640236	24.142539664305882
38	21.299875848844867	21.137065526184408	29.844656419409993	27.718402205560732
39	25.92307046572147	21.422259727751097	26.816649509359934	25.838020297167496
40	19.216036264508233	21.62549649416578	37.11324264260815	22.04522459871784
41	23.160375899654056	22.677909488947893	25.04893146060962	29.112783150788424
42	24.0572685862232	21.205547480085006	33.01360360877806	21.723580324913737
43	23.740042502993326	21.04582989082651	32.043368958676666	23.1707586475035
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5.0
1	11.5
2	18.0
3	104.5
4	191.0
5	191.0
6	300.0
7	409.0
8	454.5
9	500.0
10	749.0
11	998.0
12	998.0
13	1516.0
14	2034.0
15	4967.5
16	7901.0
17	8111.0
18	8321.0
19	8321.0
20	6818.0
21	5315.0
22	4310.5
23	3306.0
24	3217.5
25	3129.0
26	3129.0
27	3414.5
28	3700.0
29	4264.5
30	4829.0
31	5806.0
32	6783.0
33	6783.0
34	8415.5
35	10048.0
36	10983.0
37	11918.0
38	13775.5
39	15633.0
40	15633.0
41	17524.5
42	19416.0
43	22047.5
44	24679.0
45	28981.5
46	33284.0
47	33284.0
48	39132.5
49	44981.0
50	60998.0
51	77015.0
52	57929.0
53	38843.0
54	38843.0
55	48829.5
56	58816.0
57	48158.5
58	37501.0
59	25472.5
60	13444.0
61	13444.0
62	11288.0
63	9132.0
64	7070.0
65	5008.0
66	4059.0
67	3110.0
68	3110.0
69	2361.0
70	1612.0
71	1112.5
72	613.0
73	361.5
74	110.0
75	110.0
76	70.0
77	30.0
78	23.5
79	17.0
80	13.0
81	9.0
82	9.0
83	7.0
84	5.0
85	6.0
86	7.0
87	4.5
88	2.0
89	2.0
90	2.0
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	452674.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.190795141757647
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.65438095816381	12.306869844523874
2	11.086283320177301	4.033366175216601
3	4.907401785172142	2.678086216570865
4	2.954642054769567	2.1498915334214024
5	1.9309004796891127	1.756230753257311
6	1.5070738964114396	1.644892350786659
7	1.0917481328556682	1.3901836641821708
8	0.9071589046086588	1.32015534358059
9	0.7395713158054527	1.2108051268683422
>10	6.073228489890097	22.288225080300613
>50	0.6909952031088712	8.742936417819447
>100	0.40075292974679705	13.393081997198866
>500	0.029145667617948876	3.6880845818403527
>1k	0.018216042261218045	6.003216442738041
>5k	0.004857611269658145	5.499321807746855
>10k+	0.0036432084522436095	11.894652663948007
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT	22637	5.000729001444748	No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG	15674	3.4625359530257978	No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	15533	3.4313877094774603	RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC	7808	1.7248616001802621	No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC	6758	1.4929065950330702	RNA PCR Primer, Index 17 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5244	1.1584495685636904	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5084	1.1231040439698325	No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC	4794	1.0590402806434651	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	3102	0.6852613580634187	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2044	0.4515390766865338	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1828	0.4038226184848257	No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA	1824	0.40293898036997927	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1698	0.37510437975231625	No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG	1655	0.365605270017717	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1461	0.3227488214476643	No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTAT	1414	0.31236607359821855	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1339	0.2957978589448477	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	1299	0.28696147779638326	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	1279	0.28254328722215105	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	1220	0.26950962502816594	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	1116	0.24653503404215835	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	1102	0.2434423006401958	No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT	983	0.21715406672351403	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	973	0.21494497143639793	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	872	0.1926331090365252	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	857	0.18931946610585101	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	826	0.18247127071579106	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	820	0.18114581354352138	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	770	0.1701003371079408	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	764	0.16877487993567114	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	736	0.16258941313174602	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	735	0.16236850360303443	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	691	0.1526484843397235	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	677	0.14955575093776097	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	658	0.14535846989224033	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	647	0.1429284650764126	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	634	0.14005664120316166	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	626	0.13828936497346875	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	611	0.1349757220427946	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	585	0.1292320742962927	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	571	0.12613934089433013	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	570	0.12591843136561853	No Hit
ATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT	539	0.11907023597555856	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	523	0.1155356835161728	No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	515	0.1137684072864799	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	512	0.11310567870034506	No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG	482	0.10647839283899672	No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG	480	0.1060365737815735	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	465	0.10272293085089933	No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC	459	0.10139747367862967	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	4.418190574232229E-4	0.0
11	0.0	0.0	0.0	4.418190574232229E-4	0.0
12	0.0	0.0	0.0	4.418190574232229E-4	0.0
13	0.0	0.0	0.0	6.627285861348344E-4	0.0
14	0.0	0.0	0.0	6.627285861348344E-4	0.0
15	0.0	0.0	0.0	6.627285861348344E-4	0.0
16	0.0	0.0	0.0	8.836381148464458E-4	0.0
17	0.0	0.0	0.0	0.0015463667009812802	0.0
18	0.0	0.0	0.0	0.0015463667009812802	0.0
19	0.0	0.0	0.0	0.0015463667009812802	0.0
20	0.0	0.0	0.0	0.001988185758404503	0.0
21	0.0	0.0	0.0	0.0026509143445393375	0.0
22	0.0	0.0	0.0	0.0037554619880973946	0.0
23	0.0	0.0	0.0	0.005080919160367063	0.0
24	0.0	0.0	0.0	0.005964557275213509	0.0
25	0.0	0.0	0.0	0.006406376332636732	0.0
26	0.0	0.0	0.0	0.007290014447483178	0.0
27	0.0	0.0	0.0	0.009720019263310904	0.0
28	0.0	0.0	0.0	0.026067324387970152	0.0
29	0.0	0.0	0.0	0.05721556793630737	0.0
30	0.0	0.0	0.0	0.09808383074795549	0.0
31	0.0	0.0	0.0	0.17297216098119175	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAAGCA	20	0.0018407579	37.0	36
AGCACGG	20	0.0018407579	37.0	2
CTTATAC	2740	0.0	35.244526	1
GATTACG	80	0.0	34.6875	1
TTATACA	2860	0.0	33.70105	2
TATACAC	3015	0.0	32.15257	3
CGTCTTC	710	0.0	31.788734	37
TGATACA	30	3.5960617E-4	30.833334	2
TCAGAGT	55	1.850458E-8	30.272728	3
TAGGACC	80	1.8189894E-12	30.0625	4
TTGTATC	25	0.005492868	29.6	2
GTTGTAT	25	0.005492868	29.6	1
CAAGCAT	25	0.005492868	29.6	37
GAGCACG	25	0.005492868	29.6	1
TCAAGAC	25	0.005492868	29.6	2
TGCGTCG	25	0.005492868	29.6	10
ATTACTT	45	4.0012037E-6	28.777777	2
AGGACCG	60	4.310641E-8	27.750002	5
CTTAGGA	60	4.310641E-8	27.750002	2
GATACAC	40	5.9322992E-5	27.75	3
>>END_MODULE