FastQCFastQC Report
Fri 10 Feb 2017
ERR1631054.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631054.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356450
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT240626.750455884415767No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG187105.248983027072521No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT148514.166362743722822TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC99032.778229765745546No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC64971.8226960302987796RNA PCR Primer, Index 11 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT49481.388132977977276No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT47891.3435264412961145No Hit
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC47621.3359517463879926TruSeq Adapter, Index 11 (95% over 21bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA35410.9934072099873755No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG21920.6149530088371441No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA20910.5866180389956516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19260.5403282367793519No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA19210.5389255155000702No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT18500.5190068733342685No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTAT15330.4300743442278019No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT15080.42306073783139286No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA14980.4202552952728293No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT14690.41211951185299484No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC14610.4098751578061439No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG14600.4095946135502876No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC12800.35909664749614256No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG12670.35544957217000983No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT11530.3234675270023846No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG10950.30719596016271566No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG9160.25697853836442697No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG9000.2524898302707252No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT8790.2465984008977416No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG8350.23425445364006173No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG8340.23397390938420534No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8290.23257118810492355No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG8100.2272408472436527No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG8030.22527703745265815No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG7880.22106887361481276No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG7620.21377472296254732No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG7350.20620002805442558No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6730.1888062841913312No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6530.18319539907420396No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT6440.18067050077149668No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6370.17870669098050218No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC6310.177023425445364No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5980.1677654650021041No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA5960.16720437649039135No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5780.16215457988497686No Hit
ATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCT5760.16159349137326412TruSeq Adapter, Index 11 (95% over 22bp)
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5650.15850750455884416No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5460.15317716369757328No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5440.15261607518586057No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG5200.1458830130453079No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT5110.14335811474260066No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA4970.13943049516061157No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4900.13746668536961704No Hit
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT4890.13718614111376068TruSeq Adapter, Index 11 (96% over 25bp)
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA4880.13690559685790432No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG4880.13690559685790432No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4800.13466124281105346No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC4710.1321363445083462No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA4600.1290503576939262No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4580.12848926918221348No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG4580.12848926918221348No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG4490.12596437087950624No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4420.12400056108851172No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC4370.1225978398092299No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG4350.12203675129751719No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4270.11979239725066629No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG4260.11951185299480993No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG4200.11782858745967176No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA4180.11726749894795903No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4180.11726749894795903No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA4120.11558423341282087No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4030.11305933511011362No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3970.11137606957497545No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT3970.11137606957497545No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3890.10913171552812456No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC3770.10576518445784822No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA3650.1023986533875719No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA3600.10099593210829008No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG3590.10071538785243372No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3580.10043484359657737No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG3580.10043484359657737No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3570.10015429934072101No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAAC251.2319174E-437.03
TTCTACA200.001840233237.02
AACCGTC200.001840233237.016
ACAAATC200.001840233237.01
GTCGCCC200.001840233237.020
CGGCCAA200.001840233237.026
CCGGCCA200.001840233237.025
ATTACGG600.037.02
CAACATA200.001840233237.030
CCGTCGC200.001840233237.018
CATAGCC200.001840233237.033
AACATAG200.001840233237.031
GCTTAAA200.001840233237.02
TCAGAGT355.6167846E-737.03
TTACGGG600.037.03
ATTATAG200.001840233237.01
GGACAAC200.001840233237.012
ACTCAGA403.8206053E-837.01
ACATAGC200.001840233237.032
GATTACG600.037.01
Produced by FastQC (version 0.11.5)