FastQCFastQC Report
Fri 10 Feb 2017
ERR1631053.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631053.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671173
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT130481.94405913229525No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG125091.8637519685684616No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT119631.7824018546634026TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC63800.9505745910517855No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC46920.6990746052061093RNA PCR Primer, Index 11 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC32160.4791611104737527No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24770.3690553702249644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24700.3680124200466944No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG14860.22140342355845663No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11810.1759605943624073No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA11780.17551361571457733No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT10040.1495888541404377No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9340.13915935235773788No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT8400.1251540213923981No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8140.12128020644453813No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7300.10876480430529833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC17250.033.0318831
GCAACGC352.3868379E-531.7142873
CGTCTTC6350.030.29921237
GTTAGAC250.00549473429.63
GCGCTGA250.00549473429.633
TTATACA19450.029.5809762
TATACAC20450.028.7677253
TCGCCGA509.083729E-625.9000021
GGTATCA4000.024.9751
AGCAACG451.3224105E-424.6666682
GTATCAA10600.024.4339621
CCGTCTT18650.024.30294837
GCCGTCT19300.023.48445536
GTTCGAG400.001930355123.12519
TTACGGG400.001930355123.1253
GATTACG450.003824173520.5555571
TGCCGTC22550.020.18181835
ATGCCGT23750.019.16210634
GGCAATT500.007031906418.534
ACGGGTA609.2313584E-418.515
Produced by FastQC (version 0.11.5)