FastQCFastQC Report
Fri 10 Feb 2017
ERR1631052.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631052.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731227
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59020.8071364979684831No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT58060.7940078799059662No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG53050.7254929043922065No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT49540.6774913946011293No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT39100.5347176731712587No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC33630.459911901502543No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19600.26804261877638547No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17020.23275945773337142No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC16560.22646866157841547No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11450.15658612168314354No Hit
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTC10760.14714992745070957Illumina PCR Primer Index 7 (95% over 21bp)
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9720.13292725788298299No Hit
CACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCG8540.11678999818113937No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8090.1106359584643346No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT8020.10967866339727608No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7710.10543921381458836No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7370.10078949491744697No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7350.10051598204114454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCT303.5982093E-430.83333228
GGTATCA6550.030.786261
CGTCTTC1600.030.062537
ATTACGG856.91216E-1126.117652
GATTACG856.91216E-1126.117651
CCGTCTT5900.026.02542337
GTATCAA23050.025.8438191
GCCGTCT5950.025.80672536
CTTATAC9000.025.0777781
AGCAACG701.9209256E-723.7857152
TATACAC9950.023.4271353
TCCCGGA400.00193051923.12533
TTATACA9650.023.0051822
GCAACGC909.466385E-820.5555553
GACCGTT555.140636E-420.1818187
TGTGACG555.140636E-420.18181810
TGCCGTC7850.019.56050935
ATGCCGT8200.018.7256134
TCACGGT1501.2732926E-1118.51
TCTTCGC801.61536E-518.513
Produced by FastQC (version 0.11.5)