FastQCFastQC Report
Fri 10 Feb 2017
ERR1631051.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631051.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800084
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT108911.3612320706325836No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG87701.0961349058348873No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT72010.9000304967978363TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC43440.5429429909859464No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC24380.304718004609516RNA PCR Primer, Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC19960.2494738052504487No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17670.2208518105598912No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15140.1892301308362622No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG11090.13861044590318017No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG10700.13373595772443894No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA9280.11598782127876574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG700.034.3571431
CTTATAC15350.031.9381141
TTATACA16000.030.7562522
GGTATCA4050.029.2345681
TATACAC17300.028.6589583
CGTCTTC3750.027.62666537
ACGTGGG706.5865606E-926.4285736
GCAACGC509.086576E-625.9000023
GTATCAA12700.024.1811031
CCGTCTT10800.024.15277937
GCCGTCT11200.023.45535736
GTTATAC502.7011067E-422.21
CGGCCTT1001.2883902E-820.3524
TTACGGG1101.7516868E-920.1818183
AGCAACG759.2599985E-619.7333342
ATCGCTC1052.2568202E-819.3809538
TGCCGTC13850.018.83393535
TCGCCAT1002.8722025E-718.513
AACCGCC1002.8722025E-718.534
AGGACCG500.00703305718.55