##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631051.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	800084
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13683438238985	31.0	31.0	34.0	28.0	34.0
2	31.139960304168063	31.0	31.0	34.0	28.0	34.0
3	30.539754575769543	31.0	30.0	34.0	26.0	34.0
4	34.755390683978185	37.0	35.0	37.0	32.0	37.0
5	34.92385424530424	35.0	35.0	37.0	32.0	37.0
6	35.145562215967324	36.0	35.0	37.0	32.0	37.0
7	35.168434814344494	37.0	35.0	37.0	32.0	37.0
8	35.205127211642775	37.0	35.0	37.0	32.0	37.0
9	36.93315576864429	39.0	37.0	39.0	33.0	39.0
10	36.70910929352418	38.0	35.0	39.0	32.0	39.0
11	36.79341419150989	39.0	37.0	39.0	33.0	39.0
12	36.721796711345306	38.0	37.0	39.0	32.0	39.0
13	36.81376455472176	39.0	37.0	39.0	33.0	39.0
14	37.883132271111535	39.0	37.0	41.0	33.0	41.0
15	37.87796281390454	39.0	37.0	41.0	33.0	41.0
16	37.87626549212333	39.0	37.0	41.0	33.0	41.0
17	37.83925437829028	39.0	37.0	41.0	33.0	41.0
18	37.86097084806095	39.0	37.0	41.0	33.0	41.0
19	37.9046350133236	40.0	37.0	41.0	33.0	41.0
20	37.90691727368626	40.0	37.0	41.0	33.0	41.0
21	37.862346953569876	40.0	37.0	41.0	33.0	41.0
22	37.79116567760385	39.0	37.0	41.0	33.0	41.0
23	37.777299633538476	39.0	37.0	41.0	33.0	41.0
24	37.721058038905916	39.0	37.0	41.0	32.0	41.0
25	37.69811294814045	39.0	37.0	41.0	32.0	41.0
26	37.575667054959226	39.0	37.0	41.0	32.0	41.0
27	37.513717309682484	39.0	37.0	40.0	32.0	41.0
28	37.491278415766345	39.0	37.0	40.0	32.0	41.0
29	37.41879727628599	39.0	36.0	40.0	32.0	41.0
30	37.34216532264112	39.0	36.0	40.0	32.0	41.0
31	37.321658725833785	39.0	36.0	40.0	32.0	41.0
32	37.22731613180616	39.0	36.0	40.0	31.0	41.0
33	37.19235105313942	39.0	36.0	40.0	31.0	41.0
34	37.118092600276974	39.0	36.0	40.0	31.0	41.0
35	37.08138645442229	39.0	36.0	40.0	31.0	41.0
36	37.0802678218787	39.0	36.0	40.0	31.0	41.0
37	37.01814434484379	39.0	36.0	40.0	31.0	41.0
38	36.98722259162788	39.0	36.0	40.0	31.0	41.0
39	36.92409172036936	39.0	36.0	40.0	31.0	41.0
40	36.85311917248689	39.0	36.0	40.0	30.0	41.0
41	36.80081466446023	39.0	35.0	40.0	30.0	41.0
42	36.76938171491993	39.0	35.0	40.0	30.0	41.0
43	36.05796391378905	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	9.0
10	13.0
11	14.0
12	11.0
13	9.0
14	13.0
15	9.0
16	10.0
17	12.0
18	20.0
19	54.0
20	89.0
21	170.0
22	426.0
23	733.0
24	1291.0
25	2233.0
26	3421.0
27	5441.0
28	7885.0
29	11319.0
30	15479.0
31	20685.0
32	27121.0
33	34950.0
34	46826.0
35	62891.0
36	88141.0
37	129492.0
38	203672.0
39	137642.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.705060968598296	22.535508771578986	15.0377960314167	23.721634228406018
2	14.77607351228121	22.357777433369495	40.50974647661996	22.35640257772934
3	15.529619389964052	28.59799721029292	33.64284250153734	22.229540898205688
4	12.056109108543604	19.47245539218382	38.6068212837652	29.864614215507373
5	11.988741182175872	38.89441608631094	36.44417336179701	12.67266936971618
6	27.674969128241532	40.820338864419234	17.680143584923584	13.824548422415647
7	24.625789292124328	33.34849840766719	23.531904150064243	18.493808150144233
8	24.129716379780124	36.59503252158524	21.641852605476426	17.633398493158218
9	23.913614070522595	15.537618550052246	19.95203003684613	40.59673734257903
10	13.901790312017237	29.48052954439784	34.54862239464856	22.06905774893636
11	31.219721929197437	23.822998585148557	25.489448607896172	19.467830877757837
12	20.4477279885612	26.82868298828623	29.82374350693177	22.899845516220797
13	27.668969758175393	21.50586688397718	27.44011878752731	23.385044570320115
14	21.31613680563541	22.28253533378995	26.972542882997285	29.428784977577354
15	23.129946355632658	29.717629648886867	23.733632968538306	23.41879102694217
16	24.18146094660061	28.375145609710977	23.959984201658827	23.483409242029587
17	21.278390768969256	30.354562770909055	25.463076376980414	22.90397008314127
18	22.811604781497945	27.280135585763492	26.569710180431056	23.338549452307507
19	23.52040535743747	28.369271226521214	26.74869138740432	21.361632028636993
20	22.457391973842746	26.75319091495393	27.440868708785576	23.348548402417745
21	22.891721369256228	27.086280940501243	26.17887621799711	23.843121472245414
22	21.11103334149914	29.597642247564004	27.506486818884017	21.784837592052835
23	22.166797486263942	26.999665035171304	26.630953749856268	24.202583728708486
24	22.881847406022366	29.895735947725488	26.07501212372701	21.147404522525136
25	22.959714230005847	27.825453327400623	27.12865149159338	22.086180951000145
26	23.909739477354876	28.192289809569992	26.241119682433343	21.656851030641782
27	21.414626464221257	28.786477419870916	27.709965453627365	22.08893066228046
28	21.17790132036136	26.496092910244425	27.929317421670724	24.39668834772349
29	21.927697591752864	28.774103719109494	27.885322041185674	21.412876647951965
30	22.006189350118238	26.58870818564051	28.68023857494963	22.724863889291626
31	22.324655911129334	27.19251978542253	26.74131716169802	23.741507141750116
32	22.756985516520764	27.057408972057935	28.60099689532599	21.58460861609531
33	22.433894441083684	26.52059033801451	28.01443348448413	23.031081736417676
34	21.919198484159164	29.499652536483666	26.896175901530338	21.68497307782683
35	23.43066478019808	26.692322306157852	28.958459361767012	20.918553551877054
36	24.320196379380164	26.888301728318524	25.38021007794182	23.41129181435949
37	22.125301843306453	27.21339259377765	27.991060938601446	22.67024462431445
38	22.4406437324081	26.15362886896876	28.12217217192195	23.283555226701196
39	23.18994005629409	26.705195954424788	27.265262147474516	22.839601841806612
40	21.57598452162523	25.914903935086812	30.501547337529562	22.007564205758396
41	22.011813759555245	26.96004419535949	26.84718104599017	24.180960999095095
42	22.461641527639596	26.619829917858624	28.75460576639453	22.16392278810725
43	22.862224466431023	25.427705090965446	28.999205083466233	22.71086535913729
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7.0
1	13.0
2	19.0
3	68.0
4	117.0
5	117.0
6	155.5
7	194.0
8	193.0
9	192.0
10	290.5
11	389.0
12	389.0
13	635.5
14	882.0
15	1962.0
16	3042.0
17	3615.0
18	4188.0
19	4188.0
20	4697.0
21	5206.0
22	6830.5
23	8455.0
24	10968.5
25	13482.0
26	13482.0
27	16784.5
28	20087.0
29	25178.0
30	30269.0
31	33520.0
32	36771.0
33	36771.0
34	42079.0
35	47387.0
36	50116.5
37	52846.0
38	56157.0
39	59468.0
40	59468.0
41	61409.5
42	63351.0
43	61850.0
44	60349.0
45	59625.0
46	58901.0
47	58901.0
48	56785.0
49	54669.0
50	63627.5
51	72586.0
52	60927.5
53	49269.0
54	49269.0
55	51342.0
56	53415.0
57	44056.0
58	34697.0
59	28250.0
60	21803.0
61	21803.0
62	19415.5
63	17028.0
64	13548.0
65	10068.0
66	8323.0
67	6578.0
68	6578.0
69	5478.5
70	4379.0
71	4113.0
72	3847.0
73	3673.0
74	3499.0
75	3499.0
76	2540.5
77	1582.0
78	1146.0
79	710.0
80	456.5
81	203.0
82	203.0
83	143.0
84	83.0
85	60.0
86	37.0
87	29.0
88	21.0
89	21.0
90	14.0
91	7.0
92	4.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	800084.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.153697523457666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.14291753125347	44.40517967189695
2	8.764760225411585	9.142293073234969
3	2.10988926630573	3.3011557980869717
4	0.8934534553727469	1.863876050511933
5	0.5174822963452208	1.3494307578666467
6	0.3702758560308227	1.1586752997402532
7	0.2352111894520365	0.8586993260169946
8	0.19374030045796944	0.8083418422550998
9	0.15895850365945716	0.7461246346773089
>10	1.3159375344668895	14.158453013600269
>50	0.18799236617347742	6.847597333274679
>100	0.10313110506680465	8.961313009278307
>500	0.0038463815408761775	1.245870127626115
>1k	0.0016827919241333277	1.7851161192654348
>5k	4.807976926095222E-4	2.002394264699274
>10k+	2.403988463047611E-4	1.3654796779688052
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT	10891	1.3612320706325836	No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG	8770	1.0961349058348873	No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT	7201	0.9000304967978363	TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC	4344	0.5429429909859464	No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC	2438	0.304718004609516	RNA PCR Primer, Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC	1996	0.2494738052504487	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1767	0.2208518105598912	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1514	0.1892301308362622	No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG	1109	0.13861044590318017	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	1070	0.13373595772443894	No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA	928	0.11598782127876574	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.249868763779803E-4	0.0
3	0.0	0.0	0.0	1.249868763779803E-4	0.0
4	0.0	0.0	0.0	1.249868763779803E-4	0.0
5	0.0	0.0	0.0	1.249868763779803E-4	0.0
6	0.0	0.0	0.0	1.249868763779803E-4	0.0
7	0.0	0.0	0.0	1.249868763779803E-4	0.0
8	0.0	0.0	0.0	1.249868763779803E-4	0.0
9	0.0	0.0	0.0	1.249868763779803E-4	0.0
10	0.0	0.0	0.0	1.249868763779803E-4	0.0
11	1.249868763779803E-4	0.0	0.0	1.249868763779803E-4	0.0
12	1.249868763779803E-4	0.0	0.0	1.249868763779803E-4	0.0
13	1.249868763779803E-4	0.0	0.0	1.249868763779803E-4	0.0
14	1.249868763779803E-4	0.0	0.0	1.249868763779803E-4	0.0
15	1.249868763779803E-4	0.0	0.0	2.499737527559606E-4	0.0
16	1.249868763779803E-4	0.0	0.0	2.499737527559606E-4	0.0
17	1.249868763779803E-4	0.0	0.0	3.7496062913394094E-4	0.0
18	1.249868763779803E-4	0.0	0.0	3.7496062913394094E-4	0.0
19	1.249868763779803E-4	0.0	0.0	6.249343818899016E-4	0.0
20	1.249868763779803E-4	0.0	0.0	7.499212582678819E-4	0.0
21	1.249868763779803E-4	0.0	0.0	7.499212582678819E-4	0.0
22	1.249868763779803E-4	0.0	0.0	9.998950110238424E-4	0.0
23	1.249868763779803E-4	0.0	0.0	0.0013748556401577835	0.0
24	1.249868763779803E-4	0.0	0.0	0.0017498162692917243	0.0
25	1.249868763779803E-4	0.0	0.0	0.0017498162692917243	0.0
26	1.249868763779803E-4	0.0	0.0	0.0017498162692917243	0.0
27	1.249868763779803E-4	0.0	0.0	0.001999790022047685	0.0
28	1.249868763779803E-4	0.0	0.0	0.005749396313387094	0.0
29	1.249868763779803E-4	0.0	0.0	0.012873648266931973	0.0
30	1.249868763779803E-4	0.0	0.0	0.0241224671409502	0.0
31	1.249868763779803E-4	0.0	0.0	0.058618845021272764	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	70	0.0	34.357143	1
CTTATAC	1535	0.0	31.938114	1
TTATACA	1600	0.0	30.756252	2
GGTATCA	405	0.0	29.234568	1
TATACAC	1730	0.0	28.658958	3
CGTCTTC	375	0.0	27.626665	37
ACGTGGG	70	6.5865606E-9	26.42857	36
GCAACGC	50	9.086576E-6	25.900002	3
GTATCAA	1270	0.0	24.181103	1
CCGTCTT	1080	0.0	24.152779	37
GCCGTCT	1120	0.0	23.455357	36
GTTATAC	50	2.7011067E-4	22.2	1
CGGCCTT	100	1.2883902E-8	20.35	24
TTACGGG	110	1.7516868E-9	20.181818	3
AGCAACG	75	9.2599985E-6	19.733334	2
ATCGCTC	105	2.2568202E-8	19.380953	8
TGCCGTC	1385	0.0	18.833935	35
TCGCCAT	100	2.8722025E-7	18.5	13
AACCGCC	100	2.8722025E-7	18.5	34
AGGACCG	50	0.007033057	18.5	5
>>END_MODULE