FastQCFastQC Report
Fri 10 Feb 2017
ERR1631050.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631050.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375973
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT151681.1023472117548818No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG110670.8043035728171992No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT79780.5798078886722341RNA PCR Primer, Index 29 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT70780.5143996284810821No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT66350.48220422929810397No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC60130.4369998539215522No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA39300.28561606950136376No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA32170.23379819226104No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC29290.21286754899987134RNA PCR Primer, Index 29 (95% over 21bp)
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC26480.19244563665130057RNA PCR Primer, Index 29 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23600.17151499339013193No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA19880.14447957917778909No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT18870.1371393188674487No Hit
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG15860.11526388962574119No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT15180.11032193218907639No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT14460.10508927137378421No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA14210.10327237525736334No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC13940.10131012745162878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC401.6003705E-632.3753
CTTATAC18250.030.4109611
GGTATCA11850.030.2869191
GATTACG1700.029.382351
TTATACA19250.028.638962
TATACAC19850.028.6120913
CCGTCTT12400.027.15322737
TTACGGG1950.026.56413
CGTCTTC3750.026.14666637
GCCGTCT13050.025.94252836
GTATCAA35850.024.3570441
CGAACGC701.9234903E-723.78571530
AGGACCG652.6834841E-622.769235
GCAACGC502.7024772E-422.23
AAGCCGA1201.0913936E-1121.58333436
GCTTTAT8550.021.4210531
TGCCGTC16100.021.0279535
TAAGCCG1151.3460522E-1020.91304435
TACGAAC806.962164E-720.812528
ACGAACG806.962164E-720.812529
Produced by FastQC (version 0.11.5)