##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631050.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1375973
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.434074651174114	31.0	31.0	34.0	28.0	34.0
2	31.413928180276795	31.0	31.0	34.0	28.0	34.0
3	30.838307873773687	31.0	31.0	34.0	26.0	34.0
4	35.00095932114947	37.0	35.0	37.0	32.0	37.0
5	35.247566631031276	37.0	35.0	37.0	33.0	37.0
6	35.46002864881796	37.0	35.0	37.0	33.0	37.0
7	35.480107531179755	37.0	35.0	37.0	33.0	37.0
8	35.50307309809131	37.0	35.0	37.0	33.0	37.0
9	37.26792458863655	39.0	37.0	39.0	34.0	39.0
10	37.09788782192674	39.0	37.0	39.0	33.0	39.0
11	37.166471289770946	39.0	37.0	39.0	33.0	39.0
12	37.098610219822625	39.0	37.0	39.0	33.0	39.0
13	37.17033183063912	39.0	37.0	39.0	33.0	39.0
14	38.285240335384486	40.0	38.0	41.0	34.0	41.0
15	38.280260586508604	40.0	38.0	41.0	33.0	41.0
16	38.290796403708505	40.0	38.0	41.0	33.0	41.0
17	38.28675489998714	40.0	38.0	41.0	33.0	41.0
18	38.32202594091599	40.0	38.0	41.0	34.0	41.0
19	38.35617632032024	40.0	38.0	41.0	34.0	41.0
20	38.35204324503461	40.0	38.0	41.0	34.0	41.0
21	38.29699347298239	40.0	38.0	41.0	34.0	41.0
22	38.22997471607364	40.0	38.0	41.0	34.0	41.0
23	38.22337066206968	40.0	38.0	41.0	34.0	41.0
24	38.191460152197756	40.0	38.0	41.0	34.0	41.0
25	38.162405076262395	40.0	37.0	41.0	33.0	41.0
26	38.05764066591423	40.0	37.0	41.0	33.0	41.0
27	37.98363630681707	40.0	37.0	41.0	33.0	41.0
28	37.95466408134462	40.0	37.0	41.0	33.0	41.0
29	37.89029508573206	40.0	37.0	41.0	33.0	41.0
30	37.799566561262466	40.0	37.0	41.0	33.0	41.0
31	37.75550028961324	40.0	37.0	41.0	33.0	41.0
32	37.66737210686547	40.0	37.0	41.0	33.0	41.0
33	37.64293267382427	40.0	37.0	41.0	33.0	41.0
34	37.5777489819931	40.0	37.0	41.0	32.0	41.0
35	37.535067185184595	40.0	37.0	41.0	32.0	41.0
36	37.475173568086	39.0	37.0	41.0	32.0	41.0
37	37.4176440962141	39.0	36.0	41.0	32.0	41.0
38	37.372018927696985	39.0	36.0	41.0	32.0	41.0
39	37.287638638258166	39.0	36.0	41.0	31.0	41.0
40	37.20164421830952	39.0	36.0	41.0	31.0	41.0
41	37.128014139812336	39.0	36.0	41.0	31.0	41.0
42	37.049221169310734	39.0	36.0	41.0	31.0	41.0
43	36.42496909459706	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	11.0
11	12.0
12	12.0
13	15.0
14	9.0
15	2.0
16	11.0
17	10.0
18	28.0
19	58.0
20	142.0
21	213.0
22	497.0
23	948.0
24	1743.0
25	2966.0
26	4741.0
27	7751.0
28	11431.0
29	16458.0
30	22409.0
31	30318.0
32	39391.0
33	52143.0
34	69149.0
35	93383.0
36	131012.0
37	195732.0
38	335709.0
39	359662.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.29395925646797	21.443007965999332	15.173771578366727	23.08926119916597
2	14.317432100775232	22.9035017402231	40.461331726712665	22.317734432289004
3	15.20611232923902	28.60128796131901	33.79368635867128	22.39891335077069
4	11.579515005018267	18.773115460841165	39.90390799819473	29.743461535945837
5	12.63425953852292	38.179818935400625	35.56603218231753	13.619889343758926
6	27.33207701023203	41.184020325980235	17.40811774649648	14.075784917291257
7	24.969312624593652	32.812053724891406	23.272186300167228	18.946447350347718
8	25.16430191580794	34.49311868764867	22.017365166322303	18.325214230221086
9	25.258489810483198	14.707628710737783	20.311009009624463	39.72287246915455
10	14.97849158377381	29.09388483640304	33.24687330347325	22.680750276349897
11	32.45543335516031	23.84748828647074	24.82468769372655	18.872390664642403
12	22.05835434270876	26.609461086809116	29.52623343626655	21.80595113421557
13	29.328918518023244	21.623680115816228	26.375517542858763	22.671883823301766
14	21.79504975751704	22.016493056186423	28.399903195774918	27.788553990521613
15	23.772850194008164	29.01008958751371	24.248513597287154	22.96854662119097
16	23.03896951466344	27.832232173160378	24.905139853761664	24.22365845841452
17	21.78298556730401	28.764808611796887	25.84025994696117	23.611945873937934
18	23.827575105034764	25.769619025954725	27.76340814827035	22.63939772074016
19	25.175203292506467	26.022603641205166	27.780486971764706	21.02170609452366
20	24.995475928670114	25.52281185750011	26.750234197909407	22.73147801592037
21	24.047637562655662	26.11337577118156	27.1637597540068	22.67522691215598
22	22.174563018315038	29.33480526144045	27.38498502514221	21.1056466951023
23	23.553950549901778	25.5455593968777	27.684990911885627	23.215499141334895
24	22.626170717012617	26.815061051343303	27.768640809085642	22.790127422558438
25	22.700372754407244	27.918571076612697	27.568055477832775	21.813000691147284
26	23.31135858043726	27.155184004337297	27.699380729127682	21.834076686097763
27	23.025161104178643	27.947496062786115	27.480699112555264	21.54664372047998
28	22.009952230167308	26.46556291438858	28.053966175208377	23.47051868023573
29	22.279434262154854	27.396177105219362	28.10905446545826	22.215334167167526
30	24.204472035425116	26.43002442635139	28.027512167753294	21.337991370470206
31	23.0582286134975	26.294702003600364	26.591510153178877	24.055559229723258
32	21.624116170884168	27.62132687196624	28.73675573575935	22.017801221390247
33	22.266497961805936	27.16673946363773	28.832833202395687	21.733929372160645
34	21.91823531421038	26.7463823781426	27.740079202135508	23.595303105511515
35	21.47709293714339	26.58431524455785	30.201537384817872	21.73705443348089
36	22.68067760050524	27.683755422526456	26.992099408927356	22.643467568040943
37	22.605894156353358	26.6874422681259	28.226789333802333	22.479874241718406
38	22.41410260230397	25.79091304843918	28.405063180745554	23.3899211685113
39	23.396534670375072	25.848108938184104	27.81718827331641	22.93816811812441
40	21.766633502256223	25.894403451230513	30.172321695265826	22.166641351247446
41	22.479583538339778	25.59832206009856	27.408241295432394	24.513853106129265
42	22.369406957840017	25.7247053539568	29.045119344638305	22.86076834356488
43	22.79165361529623	25.376079327137962	29.112344500945873	22.719922556619935
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	22.0
1	37.0
2	52.0
3	213.5
4	375.0
5	375.0
6	497.0
7	619.0
8	605.0
9	591.0
10	969.0
11	1347.0
12	1347.0
13	2067.5
14	2788.0
15	6704.0
16	10620.0
17	11806.0
18	12992.0
19	12992.0
20	12565.0
21	12138.0
22	14365.5
23	16593.0
24	21069.5
25	25546.0
26	25546.0
27	30852.5
28	36159.0
29	42206.5
30	48254.0
31	54135.5
32	60017.0
33	60017.0
34	65679.0
35	71341.0
36	75679.5
37	80018.0
38	82946.5
39	85875.0
40	85875.0
41	87591.0
42	89307.0
43	91810.0
44	94313.0
45	95735.0
46	97157.0
47	97157.0
48	100436.0
49	103715.0
50	112122.5
51	120530.0
52	106118.0
53	91706.0
54	91706.0
55	95501.0
56	99296.0
57	85536.0
58	71776.0
59	59931.0
60	48086.0
61	48086.0
62	41936.0
63	35786.0
64	30176.5
65	24567.0
66	19967.5
67	15368.0
68	15368.0
69	12401.0
70	9434.0
71	7669.5
72	5905.0
73	4002.5
74	2100.0
75	2100.0
76	1451.0
77	802.0
78	583.5
79	365.0
80	255.5
81	146.0
82	146.0
83	135.5
84	125.0
85	98.5
86	72.0
87	60.5
88	49.0
89	49.0
90	35.0
91	21.0
92	10.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1375973.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.46435328080409
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.14637534512207	35.38092085088462
2	14.219558814632958	13.214052085205552
3	4.244452549108247	5.916472281761251
4	1.722284854868213	3.2009940778710027
5	0.8402848714529179	1.952164656185171
6	0.51324061656215	1.430843599560087
7	0.3287810811909419	1.0693620215950461
8	0.232812503512714	0.865398592912255
9	0.17261980775401015	0.7218600957672133
>10	1.2745260687915498	12.003255963681726
>50	0.17220936367328202	5.643071026806319
>100	0.12339832216420002	10.451885804350983
>500	0.005673480700171645	1.7744192410883346
>1k	0.0028367403500858227	2.4255473317022944
>5k	6.303867444635161E-4	2.028660888705481
>10k+	3.151933722317581E-4	1.9210914819227658
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT	15168	1.1023472117548818	No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG	11067	0.8043035728171992	No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	7978	0.5798078886722341	RNA PCR Primer, Index 29 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7078	0.5143996284810821	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6635	0.48220422929810397	No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC	6013	0.4369998539215522	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	3930	0.28561606950136376	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	3217	0.23379819226104	No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC	2929	0.21286754899987134	RNA PCR Primer, Index 29 (95% over 21bp)
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC	2648	0.19244563665130057	RNA PCR Primer, Index 29 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2360	0.17151499339013193	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1988	0.14447957917778909	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1887	0.1371393188674487	No Hit
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG	1586	0.11526388962574119	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1518	0.11032193218907639	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	1446	0.10508927137378421	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1421	0.10327237525736334	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	1394	0.10131012745162878	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	7.267584465683557E-5	0.0
7	0.0	0.0	0.0	7.267584465683557E-5	0.0
8	0.0	0.0	0.0	7.267584465683557E-5	0.0
9	0.0	0.0	0.0	7.267584465683557E-5	0.0
10	0.0	0.0	0.0	1.4535168931367114E-4	0.0
11	0.0	0.0	0.0	2.1802753397050668E-4	0.0
12	0.0	0.0	0.0	2.1802753397050668E-4	0.0
13	0.0	0.0	0.0	2.9070337862734227E-4	0.0
14	0.0	0.0	0.0	2.9070337862734227E-4	0.0
15	0.0	0.0	0.0	3.633792232841778E-4	0.0
16	0.0	0.0	0.0	4.3605506794101336E-4	0.0
17	0.0	0.0	0.0	5.08730912597849E-4	0.0
18	0.0	0.0	0.0	5.08730912597849E-4	0.0
19	0.0	0.0	0.0	5.08730912597849E-4	0.0
20	0.0	0.0	0.0	5.08730912597849E-4	0.0
21	0.0	0.0	0.0	0.001162813514509369	0.0
22	0.0	0.0	0.0	0.001671544427107218	0.0
23	0.0	0.0	0.0	0.0022529511843619025	0.0
24	0.0	0.0	0.0	0.002834357941616587	0.0
25	0.0	0.0	0.0	0.0030523854755870935	0.0
26	0.0	0.0	0.0	0.0035611163881849425	0.0
27	0.0	0.0	0.0	0.005159984970635325	0.0
28	0.0	0.0	0.0	0.012863624504259895	0.0
29	0.0	0.0	0.0	0.030378503066557264	0.0
30	0.0	0.0	0.0	0.054652235181940345	0.0
31	0.0	0.0	0.0	0.11126671816961525	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACGC	40	1.6003705E-6	32.375	3
CTTATAC	1825	0.0	30.410961	1
GGTATCA	1185	0.0	30.286919	1
GATTACG	170	0.0	29.38235	1
TTATACA	1925	0.0	28.63896	2
TATACAC	1985	0.0	28.612091	3
CCGTCTT	1240	0.0	27.153227	37
TTACGGG	195	0.0	26.5641	3
CGTCTTC	375	0.0	26.146666	37
GCCGTCT	1305	0.0	25.942528	36
GTATCAA	3585	0.0	24.357044	1
CGAACGC	70	1.9234903E-7	23.785715	30
AGGACCG	65	2.6834841E-6	22.76923	5
GCAACGC	50	2.7024772E-4	22.2	3
AAGCCGA	120	1.0913936E-11	21.583334	36
GCTTTAT	855	0.0	21.421053	1
TGCCGTC	1610	0.0	21.02795	35
TAAGCCG	115	1.3460522E-10	20.913044	35
TACGAAC	80	6.962164E-7	20.8125	28
ACGAACG	80	6.962164E-7	20.8125	29
>>END_MODULE