FastQCFastQC Report
Fri 10 Feb 2017
ERR1631049.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631049.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951878
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT208062.1857843126955343No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG161421.6958055549135498No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT161231.6938095007973712TruSeq Adapter, Index 8 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC86400.9076793454623387No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC66880.7026110488949214RNA PCR Primer, Index 8 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC43900.461193556317091No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28820.3027698927803773No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25240.2651600310123777No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG19810.20811490548158482No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA16150.16966459987519408No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13680.14371589636487028No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT12710.13352551482437874No Hit
ACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT11540.12123402368790959No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10370.10894253255144043No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC9620.10106337156652427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGG1150.033.7826083
CTTATAC25000.033.31
GATTACG1150.030.5652141
TTATACA27550.030.4192372
TATACAC28400.029.6390863
TAACTCG250.005495874729.65
CGTCTTC9350.029.48128537
GGTATCA4600.029.3586941
GCGGGTA601.3370045E-624.66666622
GTATCAA15500.024.58711
CCGTCTT26500.024.29433837
GCCGTCT26950.023.88868136
ATTACGG1550.022.6774182
TGCCGTC31050.020.67471735
CAACGTG450.003825332320.5555559
ATAACGC450.003825332320.5555553
ATGCCGT32850.019.76712434
TAACGCT851.2448472E-619.5882364
TCGCCGA609.2352286E-418.51
ATTAGAG1103.8495273E-818.53
Produced by FastQC (version 0.11.5)