##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631049.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	951878
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.527025522178263	31.0	31.0	34.0	28.0	34.0
2	31.55001061060346	31.0	31.0	34.0	30.0	34.0
3	30.996748532900224	31.0	31.0	34.0	27.0	34.0
4	35.10931442894993	37.0	35.0	37.0	32.0	37.0
5	35.32432937834471	37.0	35.0	37.0	33.0	37.0
6	35.52179691094867	37.0	35.0	37.0	33.0	37.0
7	35.532751045827304	37.0	35.0	37.0	33.0	37.0
8	35.5649841681392	37.0	35.0	37.0	33.0	37.0
9	37.34646877015752	39.0	37.0	39.0	34.0	39.0
10	37.18705023122711	39.0	37.0	39.0	33.0	39.0
11	37.24491058728114	39.0	37.0	39.0	34.0	39.0
12	37.179881245285635	39.0	37.0	39.0	33.0	39.0
13	37.248432046964	39.0	37.0	39.0	34.0	39.0
14	38.39163632314225	40.0	38.0	41.0	34.0	41.0
15	38.39573348685441	40.0	38.0	41.0	34.0	41.0
16	38.39603709719103	40.0	38.0	41.0	34.0	41.0
17	38.35886951899298	40.0	38.0	41.0	34.0	41.0
18	38.416327512559384	40.0	38.0	41.0	34.0	41.0
19	38.45454354444582	40.0	38.0	41.0	34.0	41.0
20	38.446507850796	40.0	38.0	41.0	34.0	41.0
21	38.42293760334833	40.0	38.0	41.0	34.0	41.0
22	38.344030432471385	40.0	38.0	41.0	34.0	41.0
23	38.33929558199685	40.0	38.0	41.0	34.0	41.0
24	38.30588478775641	40.0	38.0	41.0	34.0	41.0
25	38.26197474886487	40.0	38.0	41.0	34.0	41.0
26	38.157282761026096	40.0	38.0	41.0	33.0	41.0
27	38.095052097012434	40.0	38.0	41.0	33.0	41.0
28	38.08721916043863	40.0	37.0	41.0	33.0	41.0
29	38.05703146831842	40.0	37.0	41.0	33.0	41.0
30	37.96344909746837	40.0	37.0	41.0	33.0	41.0
31	37.93735226573153	40.0	37.0	41.0	33.0	41.0
32	37.84925168981739	40.0	37.0	41.0	33.0	41.0
33	37.767654048102806	40.0	37.0	41.0	33.0	41.0
34	37.73373898755933	40.0	37.0	41.0	33.0	41.0
35	37.72415372558248	40.0	37.0	41.0	33.0	41.0
36	37.66561891334814	40.0	37.0	41.0	33.0	41.0
37	37.62339501490737	40.0	37.0	41.0	33.0	41.0
38	37.591136679280325	40.0	37.0	41.0	33.0	41.0
39	37.472880978444714	40.0	37.0	41.0	32.0	41.0
40	37.377476945574955	40.0	37.0	41.0	32.0	41.0
41	37.35414832573082	40.0	37.0	41.0	31.0	41.0
42	37.29474155301415	39.0	36.0	41.0	31.0	41.0
43	36.617538171908585	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	11.0
10	23.0
11	20.0
12	15.0
13	15.0
14	18.0
15	7.0
16	17.0
17	18.0
18	26.0
19	41.0
20	87.0
21	156.0
22	288.0
23	622.0
24	1116.0
25	1912.0
26	3062.0
27	4928.0
28	7283.0
29	10464.0
30	14583.0
31	19657.0
32	25999.0
33	34403.0
34	45341.0
35	61312.0
36	85884.0
37	128473.0
38	227335.0
39	278759.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.24742036269354	22.46044135908173	15.58718659323989	24.70495168498484
2	13.143175911198703	21.96930699102196	41.97659784132	22.910919256459337
3	14.199298649616862	28.601774597164763	35.39917930659181	21.799747446626565
4	11.427199704163769	19.598940200319788	37.21516833039528	31.75869176512116
5	12.240854395206108	38.41962940628946	35.72127940765518	13.618236790849247
6	27.199704163768885	40.12762139685968	17.90985819611337	14.762816243258065
7	23.56898678191953	34.08052292415625	22.613192026709307	19.737298267214918
8	24.307106582986478	35.51242911381501	22.57232544506754	17.608138858130978
9	23.23470024519949	15.318664786873947	19.435473873752727	42.01116109417383
10	13.968911982417914	29.124215498204602	33.5308726538485	23.375999865528986
11	30.13316832619306	23.22871208285095	25.730293167821927	20.907826423134058
12	19.67226892521941	26.852390747553784	29.214458155351842	24.260882171874968
13	28.5702579532251	20.719777114294057	27.333019567633666	23.376945364847177
14	21.426380271421337	23.079323190576943	26.340035172574638	29.154261365427082
15	23.86261684795741	29.090282578229566	22.8861261632268	24.16097441058623
16	24.94836523167885	27.748408934758444	23.316748574922418	23.98647725864029
17	20.482561840908183	31.50708389100284	24.739304826879074	23.271049441209904
18	23.95170389482686	25.750568875423113	25.769478861786908	24.52824836796312
19	24.67007326569161	27.25601390094109	25.897646547141544	22.176266286225758
20	21.964579494431007	25.541088248704142	26.601413206314255	25.892919050550596
21	23.701146575506524	25.762860366559583	26.966270887655767	23.569722170278123
22	20.166660013152946	31.31829919380425	25.521758040421144	22.99328275262166
23	22.21734297882712	27.30591525384556	27.022265458388574	23.45447630893875
24	24.584453049655522	27.384811919174517	27.207688380233602	20.82304665093636
25	22.84095230691328	28.736770888706324	25.664423381988026	22.757853422392365
26	24.632043182004416	28.038572169962954	26.71623884573443	20.613145802298195
27	21.041562048918035	29.416164676565693	26.273009776462953	23.269263498053324
28	20.119595158203047	26.965010221898183	29.639197460178718	23.27619715972005
29	23.119769550299512	26.035059114718486	26.820874103614116	24.024297231367886
30	20.96192999523048	27.931940857967092	27.84684591933	23.259283227472427
31	21.767705525287905	26.279733327170078	29.576059116819593	22.376502030722424
32	23.341226501715557	26.58187288707166	27.160833636243296	22.916066974969482
33	20.31720451570474	29.958986340686515	28.98470182103169	20.739107322577052
34	20.682587474445256	28.78457113201482	28.868510460374125	21.664330933165804
35	23.31380702148805	25.903949875929477	30.52912242955505	20.253120673027425
36	22.525680812036835	28.45648286860291	25.386446582440186	23.631389736920067
37	22.026247061072954	27.18047901096569	27.78969573831941	23.00357818964195
38	21.964684549910807	25.285278155393865	28.685188648125077	24.06484864657025
39	23.998033361418162	25.740063327443224	26.90901565116538	23.35288765997323
40	20.648759609950016	24.974208879709376	32.3189526388886	22.058078871452015
41	21.956175056047098	26.304631475882413	26.75132737598726	24.98786609208323
42	22.894635657090507	24.931661410390827	30.278144888315516	21.895558044203145
43	22.350868493651497	24.777965243445063	30.077068699980458	22.79409756292298
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12.0
1	13.5
2	15.0
3	77.5
4	140.0
5	140.0
6	222.5
7	305.0
8	306.5
9	308.0
10	458.5
11	609.0
12	609.0
13	943.0
14	1277.0
15	3004.0
16	4731.0
17	5553.0
18	6375.0
19	6375.0
20	6708.5
21	7042.0
22	8842.0
23	10642.0
24	13850.5
25	17059.0
26	17059.0
27	20936.0
28	24813.0
29	30260.0
30	35707.0
31	39374.0
32	43041.0
33	43041.0
34	46750.5
35	50460.0
36	53363.5
37	56267.0
38	59938.5
39	63610.0
40	63610.0
41	65855.5
42	68101.0
43	65193.5
44	62286.0
45	63778.0
46	65270.0
47	65270.0
48	66724.5
49	68179.0
50	84744.5
51	101310.0
52	82879.5
53	64449.0
54	64449.0
55	69825.5
56	75202.0
57	62866.0
58	50530.0
59	37762.5
60	24995.0
61	24995.0
62	21474.0
63	17953.0
64	14998.5
65	12044.0
66	10039.0
67	8034.0
68	8034.0
69	6410.0
70	4786.0
71	3859.0
72	2932.0
73	2265.0
74	1598.0
75	1598.0
76	1254.5
77	911.0
78	703.5
79	496.0
80	349.0
81	202.0
82	202.0
83	153.5
84	105.0
85	74.5
86	44.0
87	35.0
88	26.0
89	26.0
90	17.5
91	9.0
92	6.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	951878.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.09215313360211
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.24863744505323	40.19827035660523
2	12.023292116622388	12.045451797517593
3	3.320932466552843	4.990579729827479
4	1.3083644531800023	2.62155170173017
5	0.6835446407622112	1.7120111409356862
6	0.42082281587650494	1.2647952560999716
7	0.29127212207485015	1.0213313419765848
8	0.2001230404911662	0.8019675191876442
9	0.16011412700547673	0.7218415231930221
>10	1.1055328245413407	10.822453781260347
>50	0.139651932040692	4.877475035561595
>100	0.08911503588302404	7.924113826000176
>500	0.005661425798510257	1.899968119511032
>1k	0.0018871419328367525	1.9139377010188525
>5k	4.193648739637227E-4	1.6099680101644702
>10k+	6.29047310945584E-4	5.574283159410138
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT	20806	2.1857843126955343	No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG	16142	1.6958055549135498	No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	16123	1.6938095007973712	TruSeq Adapter, Index 8 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC	8640	0.9076793454623387	No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC	6688	0.7026110488949214	RNA PCR Primer, Index 8 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC	4390	0.461193556317091	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2882	0.3027698927803773	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2524	0.2651600310123777	No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG	1981	0.20811490548158482	No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA	1615	0.16966459987519408	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1368	0.14371589636487028	No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT	1271	0.13352551482437874	No Hit
ACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT	1154	0.12123402368790959	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1037	0.10894253255144043	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	962	0.10106337156652427	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.050554797988818E-4	0.0
6	0.0	0.0	0.0	1.050554797988818E-4	0.0
7	0.0	0.0	0.0	1.050554797988818E-4	0.0
8	0.0	0.0	0.0	1.050554797988818E-4	0.0
9	0.0	0.0	0.0	1.050554797988818E-4	0.0
10	1.050554797988818E-4	0.0	0.0	1.050554797988818E-4	0.0
11	1.050554797988818E-4	0.0	0.0	2.101109595977636E-4	0.0
12	1.050554797988818E-4	0.0	0.0	2.101109595977636E-4	0.0
13	1.050554797988818E-4	0.0	0.0	2.101109595977636E-4	0.0
14	1.050554797988818E-4	0.0	0.0	2.101109595977636E-4	0.0
15	2.101109595977636E-4	0.0	0.0	2.101109595977636E-4	0.0
16	2.101109595977636E-4	0.0	0.0	5.252773989944089E-4	0.0
17	4.202219191955272E-4	0.0	0.0	5.252773989944089E-4	0.0
18	4.202219191955272E-4	0.0	0.0	5.252773989944089E-4	0.0
19	4.202219191955272E-4	0.0	0.0	6.303328787932908E-4	0.0
20	4.202219191955272E-4	0.0	0.0	8.404438383910544E-4	0.0
21	4.202219191955272E-4	0.0	0.0	9.454993181899361E-4	0.0
22	4.202219191955272E-4	0.0	0.0	0.0012606657575865816	0.0
23	4.202219191955272E-4	0.0	0.0	0.0016808876767821087	0.0
24	4.202219191955272E-4	0.0	0.0	0.002416276035374281	0.0
25	4.202219191955272E-4	0.0	0.0	0.0027314424747709267	0.0
26	4.202219191955272E-4	0.0	0.0	0.003046608914167572	0.0
27	6.303328787932908E-4	0.0	0.0	0.0036769417929608627	0.0
28	6.303328787932908E-4	0.0	0.0	0.008299382904111662	0.0
29	6.303328787932908E-4	0.0	0.0	0.018489764444603193	0.0
30	6.303328787932908E-4	0.0	0.0	0.03183181037906118	0.0
31	6.303328787932908E-4	0.0	0.0	0.07007200502585416	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGGG	115	0.0	33.782608	3
CTTATAC	2500	0.0	33.3	1
GATTACG	115	0.0	30.565214	1
TTATACA	2755	0.0	30.419237	2
TATACAC	2840	0.0	29.639086	3
TAACTCG	25	0.0054958747	29.6	5
CGTCTTC	935	0.0	29.481285	37
GGTATCA	460	0.0	29.358694	1
GCGGGTA	60	1.3370045E-6	24.666666	22
GTATCAA	1550	0.0	24.5871	1
CCGTCTT	2650	0.0	24.294338	37
GCCGTCT	2695	0.0	23.888681	36
ATTACGG	155	0.0	22.677418	2
TGCCGTC	3105	0.0	20.674717	35
CAACGTG	45	0.0038253323	20.555555	9
ATAACGC	45	0.0038253323	20.555555	3
ATGCCGT	3285	0.0	19.767124	34
TAACGCT	85	1.2448472E-6	19.588236	4
TCGCCGA	60	9.2352286E-4	18.5	1
ATTAGAG	110	3.8495273E-8	18.5	3
>>END_MODULE