FastQCFastQC Report
Fri 10 Feb 2017
ERR1631048.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631048.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834993
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT221752.655710886199046No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG169922.0349871196525No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT132781.5901929716776069TruSeq Adapter, Index 6 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC95401.1425245481099842No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT81580.9770141785619759No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT80660.9659961221231795No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA60500.7245569723338998No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC48390.5795258163840894RNA PCR Primer, Index 6 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC46870.5613220709634692No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA39490.47293809648703644No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT31840.381320561968783No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29720.3559311275663389No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT26100.3125774707093353No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT25090.3004815609232652No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG24770.29664919346629254No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA23670.28347543033294886No Hit
CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCG23400.2802418702911282No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC23210.2779664021135506No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG21940.2627566937686903No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC21620.2589243263117176No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG17920.2146125775904708No Hit
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTA17720.21221734792986288No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG16840.20167833742318797No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT16210.194133363992273No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG15600.1868279135274188No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG14820.17748651785104785No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG14730.1764086645037743No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG14470.17329486594498397No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG14440.17293558149589278No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC13680.16383370878558262No Hit
TATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTAT13530.1620372865401267No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG13440.1609594331928531No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG13410.16060014874376194No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA13090.15676778128678923No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG11750.1407197425607161No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC11450.1371268980698042No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA11370.136168806205561No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11200.13413286099404426No Hit
ACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT10660.12766574091040284No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT10450.12515074976676452No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA10190.12203695120797418No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG9960.11928243709827507No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT9790.11724649188675834No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG9360.11209674811645128No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8960.1073062887952354No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC8900.10658771989705304No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8720.10443201320250589No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG8540.10227630650795874No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA8540.10227630650795874No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG8470.10143797612674599No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG8370.10024036129644201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC26200.033.04581
TTATACA26950.032.0575142
ATTACGG1050.031.7142892
TATACAC27750.031.23
CGTCTTC6200.030.13709837
CGTTACG250.00549549429.59999816
GATTACG1200.027.7500021
CCGTCTT19750.025.66582337
GTTGTAT659.3854396E-825.6153851
GCCGTCT20000.025.067536
GGTATCA11200.024.6116071
GTATCAA32800.024.3658541
TAGACTT400.00193074723.1250025
TTATAGC400.00193074723.1250023
TATACCG400.00193074723.1250025
GACCGTT1054.0017767E-1122.9047647
TCGACAT909.470568E-820.55555523
TAGGACC1450.020.4137944
TGCCGTC25400.019.7381935
TACACAT46250.019.525
Produced by FastQC (version 0.11.5)