##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631048.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	834993
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.429469468606325	31.0	31.0	34.0	28.0	34.0
2	31.409312413397476	31.0	31.0	34.0	28.0	34.0
3	30.83424292179695	31.0	31.0	34.0	26.0	34.0
4	34.984403462064954	37.0	35.0	37.0	32.0	37.0
5	35.23848702923258	37.0	35.0	37.0	33.0	37.0
6	35.455426572438334	37.0	35.0	37.0	33.0	37.0
7	35.467367989911295	37.0	35.0	37.0	33.0	37.0
8	35.487584925861654	37.0	35.0	37.0	33.0	37.0
9	37.27444900735695	39.0	37.0	39.0	34.0	39.0
10	37.1181650624616	39.0	37.0	39.0	33.0	39.0
11	37.1835272870551	39.0	37.0	39.0	33.0	39.0
12	37.09717806017535	39.0	37.0	39.0	33.0	39.0
13	37.169994239472665	39.0	37.0	39.0	33.0	39.0
14	38.18892373948045	40.0	38.0	41.0	33.0	41.0
15	38.19553457334373	40.0	37.0	41.0	33.0	41.0
16	38.206266399838086	40.0	38.0	41.0	33.0	41.0
17	38.223365944385165	40.0	38.0	41.0	33.0	41.0
18	38.28896529671506	40.0	38.0	41.0	33.0	41.0
19	38.29285395206906	40.0	38.0	41.0	34.0	41.0
20	38.298812085849825	40.0	38.0	41.0	34.0	41.0
21	38.20413344782531	40.0	38.0	41.0	34.0	41.0
22	38.128462154772556	40.0	37.0	41.0	33.0	41.0
23	38.13121187842293	40.0	37.0	41.0	33.0	41.0
24	38.05079204256802	40.0	37.0	41.0	33.0	41.0
25	38.00161558240608	40.0	37.0	41.0	33.0	41.0
26	37.85318918841236	40.0	37.0	41.0	33.0	41.0
27	37.765942948024716	40.0	37.0	41.0	33.0	41.0
28	37.73394747021831	40.0	37.0	41.0	33.0	41.0
29	37.64977191425557	40.0	37.0	41.0	32.0	41.0
30	37.47165664861861	39.0	36.0	41.0	32.0	41.0
31	37.33338483077104	39.0	36.0	41.0	32.0	41.0
32	37.22689890813456	39.0	36.0	41.0	32.0	41.0
33	37.155535435626405	39.0	36.0	41.0	31.0	41.0
34	37.060120264481256	39.0	35.0	41.0	31.0	41.0
35	37.01087074981467	39.0	35.0	41.0	31.0	41.0
36	36.870701910075894	39.0	35.0	41.0	31.0	41.0
37	36.80841276513695	39.0	35.0	41.0	31.0	41.0
38	36.72659890561957	39.0	35.0	41.0	31.0	41.0
39	36.56992573590437	39.0	35.0	40.0	30.0	41.0
40	36.37648339566918	39.0	35.0	40.0	30.0	41.0
41	36.278181972783	39.0	35.0	40.0	30.0	41.0
42	36.10242960120624	39.0	35.0	40.0	29.0	41.0
43	35.28840241774482	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	8.0
10	17.0
11	25.0
12	20.0
13	19.0
14	7.0
15	11.0
16	15.0
17	17.0
18	29.0
19	60.0
20	115.0
21	225.0
22	443.0
23	875.0
24	1575.0
25	2524.0
26	4110.0
27	6183.0
28	8742.0
29	12141.0
30	15852.0
31	20573.0
32	26558.0
33	34561.0
34	45227.0
35	61353.0
36	82460.0
37	121244.0
38	190777.0
39	199224.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.61075122785461	20.938379124136368	15.474620745323614	23.976248902685413
2	14.113531490683156	22.402223731216907	41.0712425134103	22.413002264689645
3	15.245037982354342	29.333539323084146	33.665671448742685	21.75575124581883
4	11.472671028379878	19.145909007620425	35.53227392325445	33.849146040745254
5	14.005386871506706	37.182706920896344	32.54242849940059	16.269477708196355
6	27.632686741086452	38.92008675521831	16.67499008973728	16.772236413957962
7	24.90260397392553	32.113203344219656	20.24639727518674	22.73779540666808
8	26.294352168221767	31.98410046551288	22.588812121778265	19.13273524448708
9	24.29792824610506	13.217356313166698	18.527460709251457	43.957254731476795
10	16.106721852758046	27.85244906244723	30.132827460829013	25.90800162396571
11	32.94758159649243	22.28234248670348	23.56247297881539	21.2076029379887
12	22.064975395003312	25.81758170427776	26.74681105111061	25.370631849608323
13	31.864338982482487	19.976335130953192	25.62524476253094	22.534081124033374
14	22.680908702228642	22.358271266944755	26.727289929376653	28.23353010144995
15	26.268603449370236	26.163812151718634	22.567734100764916	24.99985029814621
16	24.866196483084288	25.303325896145235	22.048089025896026	27.782388594874448
17	21.46413203463981	29.081680924271218	23.473250673957747	25.980936367131218
18	25.372787556302868	21.974076429383242	25.814108621269877	26.839027393044013
19	26.223333608784742	24.629188508167136	25.44177017052838	23.705707712519747
20	25.320691311184646	22.922587374983983	25.957942162389386	25.798779151441988
21	25.927163461250576	24.439007273114864	26.303094756482988	23.330734509151576
22	22.08018510334817	29.213298794121627	27.163101966124266	21.543414136405932
23	23.983075307218144	23.60726377346876	26.141177231425893	26.268483687887205
24	24.902364450959467	26.128961560156792	26.31483138181997	22.65384260706377
25	24.685835689640513	26.725493507131198	26.239381647510818	22.34928915571747
26	24.76368065361027	25.375661831895595	27.054598062498727	22.806059451995406
27	24.674578110235654	27.3535227241426	27.618914170537955	20.35298499508379
28	20.01992831077626	26.302855233516926	28.09185226702499	25.58536418868182
29	22.482703447813336	25.0872761807584	26.941183938068942	25.488836433359324
30	24.603320027832567	23.74043854259856	28.68347399319515	22.97276743637372
31	24.228825870396516	26.948010342601673	26.03830211750278	22.784861669499026
32	23.306782212545492	23.78223530017617	30.407560302900745	22.503422184377595
33	20.861492252030857	23.557922042460238	31.359424570026334	24.22116113548257
34	20.356697601057732	28.99329694979479	27.016873195344154	23.633132253803325
35	23.92451194201628	24.342958563724487	31.15930313188254	20.57322636237669
36	23.420914905873463	23.905110581765356	28.587784568253866	24.086189944107318
37	23.152529422402342	26.0198588491161	27.6210698772325	23.206541851249053
38	22.00401680014084	23.013725863570116	28.590299559397504	26.391957776891545
39	24.969909927388613	22.92234785201792	27.368013863589276	24.73972835700419
40	21.304250454794232	23.969901544084802	32.516320496099965	22.209527505021
41	24.067626914237607	23.277799933652137	26.221776709505352	26.432796442604907
42	24.094932532368535	22.86737733130697	30.003365297673156	23.034324838651344
43	24.2784071243711	22.77132862191659	29.498331123733973	23.451933129978336
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	11.0
2	20.0
3	138.0
4	256.0
5	256.0
6	378.5
7	501.0
8	563.5
9	626.0
10	974.5
11	1323.0
12	1323.0
13	2036.5
14	2750.0
15	7501.0
16	12252.0
17	12762.0
18	13272.0
19	13272.0
20	11298.0
21	9324.0
22	8457.0
23	7590.0
24	8316.5
25	9043.0
26	9043.0
27	10641.0
28	12239.0
29	13697.5
30	15156.0
31	17441.0
32	19726.0
33	19726.0
34	22387.0
35	25048.0
36	26240.5
37	27433.0
38	29557.5
39	31682.0
40	31682.0
41	34206.0
42	36730.0
43	40437.5
44	44145.0
45	51211.5
46	58278.0
47	58278.0
48	69307.0
49	80336.0
50	94976.5
51	109617.0
52	92689.0
53	75761.0
54	75761.0
55	83706.0
56	91651.0
57	78168.0
58	64685.0
59	48241.0
60	31797.0
61	31797.0
62	26770.5
63	21744.0
64	17571.5
65	13399.0
66	11033.0
67	8667.0
68	8667.0
69	6941.0
70	5215.0
71	3868.5
72	2522.0
73	1768.5
74	1015.0
75	1015.0
76	826.0
77	637.0
78	472.5
79	308.0
80	219.5
81	131.0
82	131.0
83	95.5
84	60.0
85	47.5
86	35.0
87	22.5
88	10.0
89	10.0
90	6.5
91	3.0
92	3.5
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	834993.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.91983393020945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.88755477047461	21.609115872914927
2	14.884574597856037	9.204571493750455
3	5.7896391220523125	5.370440405091035
4	2.7780497876037002	3.4358735233024027
5	1.5566829508651483	2.4066189161369396
6	0.98636695856835	1.8298981531907503
7	0.6502587224049932	1.4074124195902793
8	0.4554156000678916	1.1265099778660714
9	0.36653465968013077	1.0199871726277934
>10	2.1836694634516656	12.786878210791318
>50	0.23199133895699842	5.046992735758026
>100	0.19261129564786683	12.073288093229369
>500	0.020664774634285305	4.409970227970466
>1k	0.013256647878598121	8.114441664959982
>5k	0.0015596056327762497	3.8353961955345106
>10k+	0.0011697042245821872	6.322604937285704
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT	22175	2.655710886199046	No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG	16992	2.0349871196525	No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	13278	1.5901929716776069	TruSeq Adapter, Index 6 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC	9540	1.1425245481099842	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8158	0.9770141785619759	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8066	0.9659961221231795	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	6050	0.7245569723338998	No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC	4839	0.5795258163840894	RNA PCR Primer, Index 6 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC	4687	0.5613220709634692	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	3949	0.47293809648703644	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	3184	0.381320561968783	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2972	0.3559311275663389	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	2610	0.3125774707093353	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	2509	0.3004815609232652	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	2477	0.29664919346629254	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	2367	0.28347543033294886	No Hit
CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCG	2340	0.2802418702911282	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	2321	0.2779664021135506	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	2194	0.2627566937686903	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	2162	0.2589243263117176	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	1792	0.2146125775904708	No Hit
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTA	1772	0.21221734792986288	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	1684	0.20167833742318797	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	1621	0.194133363992273	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	1560	0.1868279135274188	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	1482	0.17748651785104785	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	1473	0.1764086645037743	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	1447	0.17329486594498397	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	1444	0.17293558149589278	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	1368	0.16383370878558262	No Hit
TATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTAT	1353	0.1620372865401267	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	1344	0.1609594331928531	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	1341	0.16060014874376194	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1309	0.15676778128678923	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	1175	0.1407197425607161	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	1145	0.1371268980698042	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	1137	0.136168806205561	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1120	0.13413286099404426	No Hit
ACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT	1066	0.12766574091040284	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	1045	0.12515074976676452	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	1019	0.12203695120797418	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	996	0.11928243709827507	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	979	0.11724649188675834	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	936	0.11209674811645128	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	896	0.1073062887952354	No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC	890	0.10658771989705304	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	872	0.10443201320250589	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	854	0.10227630650795874	No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA	854	0.10227630650795874	No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG	847	0.10143797612674599	No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	837	0.10024036129644201	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	3.5928444909119E-4	0.0
11	0.0	0.0	0.0	3.5928444909119E-4	0.0
12	0.0	0.0	0.0	3.5928444909119E-4	0.0
13	0.0	0.0	0.0	3.5928444909119E-4	0.0
14	0.0	0.0	0.0	4.7904593212158667E-4	0.0
15	0.0	0.0	0.0	4.7904593212158667E-4	0.0
16	0.0	0.0	0.0	5.988074151519833E-4	0.0
17	0.0	0.0	0.0	7.1856889818238E-4	0.0
18	0.0	0.0	0.0	7.1856889818238E-4	0.0
19	0.0	0.0	0.0	8.383303812127766E-4	0.0
20	0.0	0.0	0.0	0.0011976148303039667	0.0
21	0.0	0.0	0.0	0.0017964222454559498	0.0
22	0.0	0.0	0.0	0.0026347526266687266	0.0
23	0.0	0.0	0.0	0.00323356004182071	0.0
24	0.0	0.0	0.0	0.004311413389094279	0.0
25	0.0	0.0	0.0	0.004790459321215867	0.0
26	0.0	0.0	0.0	0.005628789702428643	0.0
27	0.0	0.0	0.0	0.007544973430914989	0.0
28	0.0	0.0	0.0	0.019281598767893864	0.0
29	0.0	0.0	0.0	0.04922196952549303	0.0
30	0.0	0.0	0.0	0.09365347972977019	0.0
31	0.0	0.0	0.0	0.16287561692133945	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2620	0.0	33.0458	1
TTATACA	2695	0.0	32.057514	2
ATTACGG	105	0.0	31.714289	2
TATACAC	2775	0.0	31.2	3
CGTCTTC	620	0.0	30.137098	37
CGTTACG	25	0.005495494	29.599998	16
GATTACG	120	0.0	27.750002	1
CCGTCTT	1975	0.0	25.665823	37
GTTGTAT	65	9.3854396E-8	25.615385	1
GCCGTCT	2000	0.0	25.0675	36
GGTATCA	1120	0.0	24.611607	1
GTATCAA	3280	0.0	24.365854	1
TAGACTT	40	0.001930747	23.125002	5
TTATAGC	40	0.001930747	23.125002	3
TATACCG	40	0.001930747	23.125002	5
GACCGTT	105	4.0017767E-11	22.904764	7
TCGACAT	90	9.470568E-8	20.555555	23
TAGGACC	145	0.0	20.413794	4
TGCCGTC	2540	0.0	19.73819	35
TACACAT	4625	0.0	19.52	5
>>END_MODULE