FastQCFastQC Report
Fri 10 Feb 2017
ERR1631047.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631047.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872067
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT216652.484327465664909No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG160571.841257609793743No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT139631.6011384446378545Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC80460.9226355314442584No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC60210.6904286023894953Illumina PCR Primer Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC39740.455698931389446No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38270.43884242839139653No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35480.4068494737216292No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA21920.25135683382125457No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG19330.2216572809199293No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA16470.18886163563120723No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14420.1653542675046757No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT13150.1507911662750683No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA12220.14012684805181252No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT11590.13290263248121992No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA10820.12407303567271782No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT10450.1198302424010999No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT10260.11765151072107992No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT9800.11237668665366309No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9790.11226201656524097No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9040.10366175993358308No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8980.10297373940305046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC25400.032.338581
CGTCTTC7650.031.92156837
GATTACG705.456968E-1231.7142851
TTATACA26200.031.0687032
GTGCGAG303.598773E-430.83333236
GGTATCA8700.029.5574721
TATACAC28000.029.4678573
CGCCGAG358.867674E-426.428572
CCGTCTT23050.024.63991437
GCCGTCT23600.024.14406836
TTACGGG952.8012437E-1023.3684223
TGCGAGA400.001930815223.12500237
GTCTTAT400.001930815223.1250021
GCAACGC502.701378E-422.23
GTATCAA22300.022.0672651
TGCCGTC28500.020.05789635
ATGCCGT29900.018.99498634
CGGTTCG1306.9485395E-1018.522
AGGACCG500.007033554418.55
CGACCCT609.2343806E-418.529
Produced by FastQC (version 0.11.5)