FastQCFastQC Report
Fri 10 Feb 2017
ERR1631046.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631046.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1078022
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT211311.9601640782841168No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT156491.451640133503769TruSeq Adapter, Index 3 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG153151.4206574633912852No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC75670.7019337267699546No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC69150.6414525863108546RNA PCR Primer, Index 3 (95% over 22bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC42940.3983221121646868No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27940.2591783841146099No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25280.23450356300706293No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG16960.15732517518195363No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA16030.14869826404284883No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15800.14656472687941435No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTAT11770.109181445276627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10880.10092558407898912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC27200.032.3751
CGTCTTC8600.032.05232637
TATACAC30300.029.9785483
TTATACA30850.029.2042162
TAACGGC601.3372792E-624.66666636
GGTATCA5450.024.4403671
CCGTCTT23950.024.25469837
CGAACTA701.92269E-723.78571529
TTACGGG952.8194336E-1023.3684223
GATTACG952.8194336E-1023.3684221
GCCGTCT25150.023.09741436
GTATCAA15650.022.3418541
TTAACGG705.1014376E-621.14285935
TGCCGTC29550.019.72081235
ATACACA47800.019.1192464
TACACAT47500.019.0452635
ATGCCGT31200.018.7371834
GCCGGTC500.007034604418.520
AACGGCC801.6165188E-518.537
CCGTTAA609.236309E-418.516
Produced by FastQC (version 0.11.5)