##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631046.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1078022
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.537489030836106	31.0	31.0	34.0	28.0	34.0
2	31.546220763583676	31.0	31.0	34.0	30.0	34.0
3	31.00169940873192	31.0	31.0	34.0	27.0	34.0
4	35.116542148490474	37.0	35.0	37.0	32.0	37.0
5	35.3371860685589	37.0	35.0	37.0	33.0	37.0
6	35.525991120774904	37.0	35.0	37.0	33.0	37.0
7	35.5422301214632	37.0	35.0	37.0	33.0	37.0
8	35.56402930552438	37.0	35.0	37.0	33.0	37.0
9	37.34835281654734	39.0	37.0	39.0	34.0	39.0
10	37.188304134795025	39.0	37.0	39.0	33.0	39.0
11	37.24479648838335	39.0	37.0	39.0	34.0	39.0
12	37.19093395125517	39.0	37.0	39.0	33.0	39.0
13	37.26141024951254	39.0	37.0	39.0	34.0	39.0
14	38.4148811434275	40.0	38.0	41.0	34.0	41.0
15	38.437968798410424	40.0	38.0	41.0	34.0	41.0
16	38.42323997098389	40.0	38.0	41.0	34.0	41.0
17	38.39398824884835	40.0	38.0	41.0	34.0	41.0
18	38.43155148967275	40.0	38.0	41.0	34.0	41.0
19	38.427946739491404	40.0	38.0	41.0	34.0	41.0
20	38.39460048125177	40.0	38.0	41.0	34.0	41.0
21	38.38224730107549	40.0	38.0	41.0	34.0	41.0
22	38.330672286836446	40.0	38.0	41.0	34.0	41.0
23	38.31811873969177	40.0	38.0	41.0	34.0	41.0
24	38.29482700724104	40.0	38.0	41.0	34.0	41.0
25	38.235237314266314	40.0	38.0	41.0	34.0	41.0
26	38.14418629675461	40.0	38.0	41.0	33.0	41.0
27	38.04236926519125	40.0	37.0	41.0	33.0	41.0
28	38.03763559556298	40.0	37.0	41.0	33.0	41.0
29	38.006941416779995	40.0	37.0	41.0	33.0	41.0
30	37.847075477123845	40.0	37.0	41.0	33.0	41.0
31	37.86432095077837	40.0	37.0	41.0	33.0	41.0
32	37.81574402006638	40.0	37.0	41.0	33.0	41.0
33	37.755238761361085	40.0	37.0	41.0	33.0	41.0
34	37.71506332894876	40.0	37.0	41.0	33.0	41.0
35	37.69486893588442	40.0	37.0	41.0	33.0	41.0
36	37.65951529746146	40.0	37.0	41.0	33.0	41.0
37	37.61580468673181	40.0	37.0	41.0	33.0	41.0
38	37.58079148663014	40.0	37.0	41.0	32.0	41.0
39	37.49280163113554	40.0	37.0	41.0	32.0	41.0
40	37.39630452810796	39.0	37.0	41.0	32.0	41.0
41	37.35798249015326	39.0	37.0	41.0	32.0	41.0
42	37.296084866542614	39.0	36.0	41.0	31.0	41.0
43	36.64421227025051	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	13.0
10	16.0
11	28.0
12	19.0
13	18.0
14	11.0
15	15.0
16	10.0
17	9.0
18	16.0
19	55.0
20	80.0
21	170.0
22	322.0
23	654.0
24	1237.0
25	2084.0
26	3451.0
27	5499.0
28	8289.0
29	11963.0
30	16821.0
31	22410.0
32	29651.0
33	38428.0
34	52024.0
35	70331.0
36	97725.0
37	146349.0
38	257573.0
39	312750.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.22389524518052	21.556053587032547	14.980863099268845	25.239188068518082
2	13.778475763945448	21.341679483350063	41.20722953705954	23.67261521564495
3	14.736990525239746	27.685334807638434	34.059880039553924	23.5177946275679
4	11.834730645571241	19.22957045403526	37.01408691102779	31.92161198936571
5	13.23266130004768	37.30489730265245	35.215051269825665	14.247390127474208
6	28.405821031481732	39.36914088951802	17.60038292353959	14.62465515546065
7	25.12277114938285	32.91621135746766	22.176356326679787	19.7846611664697
8	25.044201324277243	35.10865269911004	21.789815050156676	18.057330926456046
9	23.87836240818833	14.956837615558866	19.44301693286408	41.721783043388726
10	14.740144449742212	28.51082816491686	32.94339076568011	23.805636619660824
11	31.58033880570155	22.673748773216133	25.309873082367524	20.436039338714796
12	20.865251358506598	25.690755847283263	29.186881158269497	24.25711163594064
13	29.17714109730599	20.418507228980484	26.417457157646133	23.986894516067387
14	21.94964481244353	22.13414939583793	26.156980098736387	29.75922569298215
15	24.296813979677594	28.430959665016115	22.42421768757966	24.84800866772663
16	25.36840621063392	27.066423505271693	23.321045396105088	24.244124887989297
17	21.361159605277074	30.351977974475474	24.65589756053216	23.63096485971529
18	24.811460248492146	25.33482619093117	25.285476548716073	24.56823701186061
19	24.98882212051331	26.783683449873934	25.68862231011983	22.538872119492922
20	22.5534358296955	25.950119756368608	26.770696701922596	24.7257477120133
21	25.098003565789938	26.596024941977063	25.16859581715401	23.137375675078985
22	23.054446013161144	29.894009584219987	25.634634543636402	21.416909858982468
23	24.023813985243343	26.73581800742471	25.364881236189984	23.87548677114196
24	26.8428659155379	26.703629425002458	25.07703924409706	21.37646541536258
25	23.554435809287753	28.279478526412262	25.83221863746751	22.333867026832475
26	25.912550949795087	28.135789436579216	24.884742611931856	21.066917001693845
27	23.02791594234626	28.645148243727864	27.02848364875671	21.29845216516917
28	21.705586713443694	27.60602288264989	26.866891399247883	23.82149900465853
29	26.36161414145537	25.377682459170593	25.643168692290143	22.6175347070839
30	22.264109637836704	26.833033092089032	29.063043240304932	21.839814029769336
31	24.559981150662974	27.488307288719525	24.844483693282697	23.107227867334803
32	26.225067762995565	25.62786288220463	26.451222702319622	21.695846652480192
33	21.697238089760692	28.99606872587016	27.64470483904781	21.66198834532134
34	24.0069312129066	27.610939294374326	25.94696583186614	22.435163660852933
35	24.311470452365537	25.362654936541183	29.10831133316389	21.217563277929393
36	23.494975056167686	27.455005556472873	25.055147297550512	23.99487208980893
37	22.545365493468594	26.755761941778555	27.0204133125298	23.678459252223053
38	22.69721768201391	24.76637768060392	27.72605753871442	24.810347098667744
39	24.352749758353724	25.096612128509438	26.379146251189677	24.17149186194716
40	21.713749812155967	24.903387871490562	30.758092135410962	22.624770180942505
41	22.84220544664209	25.480370530471546	26.03889345486456	25.638530568021807
42	23.336165681219864	24.759142206745317	29.321015712109773	22.58367639992505
43	23.271695753889997	24.33308411145598	29.091428560827143	23.303791573826878
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10.0
1	15.5
2	21.0
3	81.0
4	141.0
5	141.0
6	205.0
7	269.0
8	269.5
9	270.0
10	427.0
11	584.0
12	584.0
13	906.5
14	1229.0
15	2818.0
16	4407.0
17	5117.5
18	5828.0
19	5828.0
20	6007.5
21	6187.0
22	7402.5
23	8618.0
24	11614.5
25	14611.0
26	14611.0
27	18835.5
28	23060.0
29	27347.0
30	31634.0
31	36798.0
32	41962.0
33	41962.0
34	47362.0
35	52762.0
36	57097.0
37	61432.0
38	64840.5
39	68249.0
40	68249.0
41	70022.0
42	71795.0
43	73375.0
44	74955.0
45	76239.0
46	77523.0
47	77523.0
48	79600.5
49	81678.0
50	96794.5
51	111911.0
52	90684.5
53	69458.0
54	69458.0
55	77920.5
56	86383.0
57	74636.5
58	62890.0
59	50570.0
60	38250.0
61	38250.0
62	33583.0
63	28916.0
64	24253.0
65	19590.0
66	16392.5
67	13195.0
68	13195.0
69	10946.0
70	8697.0
71	6958.0
72	5219.0
73	4074.5
74	2930.0
75	2930.0
76	2274.0
77	1618.0
78	1254.0
79	890.0
80	664.0
81	438.0
82	438.0
83	327.5
84	217.0
85	166.0
86	115.0
87	86.0
88	57.0
89	57.0
90	37.5
91	18.0
92	11.5
93	5.0
94	2.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1078022.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.18136750632446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.69348443599954	42.87232717509791
2	13.288527417448414	14.665582300801796
3	4.011651755001208	6.641052894003396
4	1.6902068486849342	3.730717011159596
5	0.8782424341165446	2.4231309258316998
6	0.5472202863629897	1.8117818237227352
7	0.3556068053479529	1.3736008869558767
8	0.25456398034207717	1.1237750842503134
9	0.18105467376198947	0.8991760042438219
>10	0.9938345046223463	10.134637096025664
>50	0.0675024307414477	2.5615829466311433
>100	0.033907905601049476	3.2377170872522205
>500	0.001846469666561647	0.7098078154672638
>1k	0.001510747909004984	1.6482213582701934
>5k	3.357217575566631E-4	1.3414376593497213
>10k+	5.035826363349947E-4	4.825451930936593
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT	21131	1.9601640782841168	No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	15649	1.451640133503769	TruSeq Adapter, Index 3 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG	15315	1.4206574633912852	No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC	7567	0.7019337267699546	No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC	6915	0.6414525863108546	RNA PCR Primer, Index 3 (95% over 22bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC	4294	0.3983221121646868	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2794	0.2591783841146099	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2528	0.23450356300706293	No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG	1696	0.15732517518195363	No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA	1603	0.14869826404284883	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1580	0.14656472687941435	No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTAT	1177	0.109181445276627	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1088	0.10092558407898912	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.276248536671793E-5	0.0
10	0.0	0.0	0.0	1.8552497073343586E-4	0.0
11	0.0	0.0	0.0	2.782874561001538E-4	0.0
12	0.0	0.0	0.0	2.782874561001538E-4	0.0
13	0.0	0.0	0.0	2.782874561001538E-4	0.0
14	0.0	0.0	0.0	2.782874561001538E-4	0.0
15	0.0	0.0	0.0	2.782874561001538E-4	0.0
16	0.0	0.0	0.0	2.782874561001538E-4	0.0
17	0.0	0.0	0.0	2.782874561001538E-4	0.0
18	0.0	0.0	0.0	2.782874561001538E-4	0.0
19	0.0	0.0	0.0	2.782874561001538E-4	0.0
20	0.0	0.0	0.0	3.710499414668717E-4	0.0
21	0.0	0.0	0.0	0.0010203873390338973	0.0
22	0.0	0.0	0.0	0.001391437280500769	0.0
23	0.0	0.0	0.0	0.0019480121927010765	0.0
24	0.0	0.0	0.0	0.0025973495902681023	0.0
25	0.0	0.0	0.0	0.0025973495902681023	0.0
26	0.0	0.0	0.0	0.002875637046368256	0.0
27	0.0	0.0	0.0	0.0037104994146687172	0.0
28	0.0	0.0	0.0	0.009276248536671793	0.0
29	0.0	0.0	0.0	0.0206860342367781	0.0
30	0.0	0.0	0.0	0.03385830715885205	0.0
31	0.0	0.0	0.0	0.07189092615920639	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2720	0.0	32.375	1
CGTCTTC	860	0.0	32.052326	37
TATACAC	3030	0.0	29.978548	3
TTATACA	3085	0.0	29.204216	2
TAACGGC	60	1.3372792E-6	24.666666	36
GGTATCA	545	0.0	24.440367	1
CCGTCTT	2395	0.0	24.254698	37
CGAACTA	70	1.92269E-7	23.785715	29
TTACGGG	95	2.8194336E-10	23.368422	3
GATTACG	95	2.8194336E-10	23.368422	1
GCCGTCT	2515	0.0	23.097414	36
GTATCAA	1565	0.0	22.341854	1
TTAACGG	70	5.1014376E-6	21.142859	35
TGCCGTC	2955	0.0	19.720812	35
ATACACA	4780	0.0	19.119246	4
TACACAT	4750	0.0	19.045263	5
ATGCCGT	3120	0.0	18.73718	34
GCCGGTC	50	0.0070346044	18.5	20
AACGGCC	80	1.6165188E-5	18.5	37
CCGTTAA	60	9.236309E-4	18.5	16
>>END_MODULE