FastQCFastQC Report
Fri 10 Feb 2017
ERR1631045.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631045.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932470
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT127161.363689984664386No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG106221.1391251193067875No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT90850.9742940791660857RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC57510.616749064313061No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37160.39851148026209965No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33830.362799875599215No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC30270.32462170364730236RNA PCR Primer, Index 41 (95% over 23bp)
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA28450.30510364944716717No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC28280.30328053449440734No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG14510.15560822332085752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12850.13780604201743754No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11490.12322112239535857No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTA9590.10284513174686584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC15100.031.4867551
CGTCTTC4600.029.7608737
TTATACA16200.029.6913592
GGTATCA6750.027.681481
TATACAC17800.027.022473
GCCGTCT14100.025.58510636
CCGTCTT14050.025.54448537
GTATCAA20950.024.0190931
GATTACG603.725317E-521.5833321
GTCTTAT806.957216E-720.81251
TGCCGTC17350.020.79250535
TATATCG555.142093E-420.1818185
TTACGGG555.142093E-420.1818183
GCTTTAT5300.019.8962271
ATGCCGT18100.019.8287334
ATTGAGC5200.019.5673086
CCGTCTG609.2350366E-418.527
TCTACAC1601.8189894E-1218.53
TTAGTAC902.1513297E-618.53
TATGCCG19600.018.31122433
Produced by FastQC (version 0.11.5)