##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631045.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	932470
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.415537229079757	31.0	31.0	34.0	28.0	34.0
2	31.41487769043508	31.0	31.0	34.0	28.0	34.0
3	30.851047218677277	31.0	31.0	34.0	27.0	34.0
4	35.00963784357674	37.0	35.0	37.0	32.0	37.0
5	35.230379529636345	37.0	35.0	37.0	32.0	37.0
6	35.43639473655989	37.0	35.0	37.0	33.0	37.0
7	35.45286818878891	37.0	35.0	37.0	33.0	37.0
8	35.472547106073115	37.0	35.0	37.0	33.0	37.0
9	37.23143157420614	39.0	37.0	39.0	34.0	39.0
10	37.0681930785977	39.0	37.0	39.0	33.0	39.0
11	37.12276748849829	39.0	37.0	39.0	33.0	39.0
12	37.060481302347526	39.0	37.0	39.0	33.0	39.0
13	37.138039829699615	39.0	37.0	39.0	33.0	39.0
14	38.259839994852385	40.0	38.0	41.0	33.0	41.0
15	38.26178858301071	40.0	38.0	41.0	33.0	41.0
16	38.258840498890045	40.0	38.0	41.0	33.0	41.0
17	38.24864606904244	40.0	38.0	41.0	33.0	41.0
18	38.280982766201596	40.0	38.0	41.0	33.0	41.0
19	38.31938292920952	40.0	38.0	41.0	34.0	41.0
20	38.32800733535663	40.0	38.0	41.0	34.0	41.0
21	38.27881647666949	40.0	38.0	41.0	34.0	41.0
22	38.20695143007282	40.0	38.0	41.0	34.0	41.0
23	38.20095767156048	40.0	38.0	41.0	34.0	41.0
24	38.15767156047916	40.0	37.0	41.0	33.0	41.0
25	38.13290079037395	40.0	37.0	41.0	33.0	41.0
26	38.03176187973876	40.0	37.0	41.0	33.0	41.0
27	37.9740956813624	40.0	37.0	41.0	33.0	41.0
28	37.93565476637318	40.0	37.0	41.0	33.0	41.0
29	37.86279344107585	40.0	37.0	41.0	33.0	41.0
30	37.80754126138106	40.0	37.0	41.0	33.0	41.0
31	37.7690392184199	40.0	37.0	41.0	33.0	41.0
32	37.68221068774331	40.0	37.0	41.0	33.0	41.0
33	37.65336364708784	40.0	37.0	41.0	33.0	41.0
34	37.58671056441494	40.0	37.0	41.0	32.0	41.0
35	37.558987420506824	40.0	37.0	41.0	32.0	41.0
36	37.5274035625811	39.0	37.0	41.0	32.0	41.0
37	37.47312406833464	39.0	37.0	41.0	32.0	41.0
38	37.43345094212146	39.0	37.0	41.0	32.0	41.0
39	37.34899996782738	39.0	36.0	41.0	32.0	41.0
40	37.2679464218688	39.0	36.0	41.0	31.0	41.0
41	37.21284009137023	39.0	36.0	41.0	31.0	41.0
42	37.159286625843194	39.0	36.0	41.0	31.0	41.0
43	36.528776260898475	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	12.0
11	9.0
12	10.0
13	7.0
14	8.0
15	6.0
16	7.0
17	13.0
18	22.0
19	33.0
20	74.0
21	166.0
22	311.0
23	687.0
24	1115.0
25	1924.0
26	3208.0
27	5110.0
28	7739.0
29	10868.0
30	15088.0
31	20490.0
32	27225.0
33	35366.0
34	47251.0
35	63490.0
36	88353.0
37	132637.0
38	229602.0
39	241632.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.97004729374672	22.017544800368913	15.3373298872886	23.675078018595773
2	14.006992182054114	22.4628138170665	40.36816197840145	23.162032022477934
3	14.94868467618261	28.313082458416893	34.16785526612116	22.570377599279333
4	11.665254646262078	18.95299580683561	39.14667496005234	30.23507458684998
5	12.385063326434095	38.0439048977447	36.101644020719164	13.46938775510204
6	27.588984096003088	40.74125709138096	17.59423895674928	14.075519855866677
7	24.62041674262979	33.008461398221925	23.263161281328085	19.107960577820197
8	25.021823758405098	34.615054639827555	22.410372451660642	17.952749150106705
9	24.607976664128607	14.98332386028505	20.095981640160005	40.31271783542634
10	14.402071916522782	29.030746297468013	33.78210559052838	22.78507619548082
11	31.60434115842869	23.664782781215482	25.203813527512946	19.52706253284288
12	21.221487018349116	26.61050757665126	29.499286840327304	22.66871856467232
13	28.928008407777195	21.25194376226581	26.614368290668867	23.205679539288127
14	21.718875674284426	22.151811854536877	27.521636084807017	28.60767638637168
15	23.667356590560555	29.206408785269232	23.645908179351615	23.4803264448186
16	23.72247900736753	27.83413943612127	24.414511995023968	24.02886956148723
17	21.211299023024868	29.785944856134783	25.68704623205036	23.315709888789986
18	23.459521485945928	25.91911804133109	26.99883106158911	23.62252941113387
19	24.06329426147758	26.65436957757354	26.67517453644621	22.607161624502663
20	23.482149559771358	25.875363282464853	26.559996568254206	24.082490589509582
21	23.946400420388862	26.092957414179548	27.29256705309554	22.66807511233605
22	21.27746737160445	30.628974658702155	26.78649179062061	21.307066179072784
23	22.931032633757653	26.6835394168177	27.116475597070146	23.2689523523545
24	23.34648835887482	27.62255085954508	26.62509249627334	22.405868285306767
25	21.590828659367055	29.142921488090767	26.52396323742319	22.742286615118985
26	23.407401846708208	26.524177721535274	26.93416410179416	23.13425632996236
27	21.579032033202143	29.12372516005877	26.691046360740827	22.606196445998265
28	21.536027968728217	26.60278614861604	28.744517249884716	23.116668632771027
29	22.16854161527985	27.739980910914024	26.609971366371038	23.48150610743509
30	21.802202751831157	27.830922174439927	27.506300470792628	22.860574602936286
31	23.20739541218484	26.08416356558388	27.82759767070254	22.880843351528735
32	21.76134352847813	29.514836938453787	27.422651667077762	21.30116786599033
33	20.99241798663764	27.822235567900304	28.6861775713964	22.499168874065653
34	23.391101054189413	27.372998595129065	27.198515769944343	22.037384580737182
35	21.400259525775628	27.387047304470926	29.637307366456827	21.57538580329662
36	22.611236822632364	26.308942915053567	26.62101729814364	24.458802964170427
37	22.257659763852995	26.79968256351411	28.393942968674597	22.548714703958304
38	22.377341898398875	25.601574313382734	28.513303377052345	23.507780411166042
39	23.82500241294626	25.96523212543031	27.288062886741667	22.921702574881767
40	21.411841667828455	25.628277585337866	30.851072956770725	22.10880779006295
41	22.128004118094953	26.09907021137409	27.162804165281457	24.6101215052495
42	22.522225916115264	25.474170750801633	29.339496176820703	22.6641071562624
43	22.7905455403391	25.242420667689043	29.373277424474782	22.59375636749708
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	22.0
1	28.0
2	34.0
3	127.5
4	221.0
5	221.0
6	279.5
7	338.0
8	348.5
9	359.0
10	559.0
11	759.0
12	759.0
13	1165.5
14	1572.0
15	3655.5
16	5739.0
17	6527.0
18	7315.0
19	7315.0
20	7420.0
21	7525.0
22	9172.0
23	10819.0
24	14107.0
25	17395.0
26	17395.0
27	21407.5
28	25420.0
29	29799.5
30	34179.0
31	38995.0
32	43811.0
33	43811.0
34	47638.0
35	51465.0
36	54487.5
37	57510.0
38	59545.0
39	61580.0
40	61580.0
41	61517.0
42	61454.0
43	62117.0
44	62780.0
45	63133.5
46	63487.0
47	63487.0
48	64065.5
49	64644.0
50	73845.0
51	83046.0
52	69728.5
53	56411.0
54	56411.0
55	61982.5
56	67554.0
57	57611.5
58	47669.0
59	39621.0
60	31573.0
61	31573.0
62	28157.5
63	24742.0
64	21036.0
65	17330.0
66	14230.0
67	11130.0
68	11130.0
69	8982.0
70	6834.0
71	5732.5
72	4631.0
73	3173.0
74	1715.0
75	1715.0
76	1211.5
77	708.0
78	530.5
79	353.0
80	267.0
81	181.0
82	181.0
83	128.0
84	75.0
85	64.5
86	54.0
87	39.0
88	24.0
89	24.0
90	17.5
91	11.0
92	6.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	932470.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.80679480905412
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.57210133623015	46.906564175596486
2	11.019201187964997	12.51931001688826
3	2.7692860194220894	4.719427880186787
4	1.0601240270954548	2.408889923174385
5	0.5580046741172663	1.5849228512536329
6	0.34110862195147673	1.162637249687805
7	0.24396962924509938	0.9701392867717158
8	0.16576613141944072	0.7533314091067894
9	0.13816782743532247	0.7063984280098046
>10	0.9560771466833077	10.800933929377708
>50	0.11128240761998061	4.3607788716275495
>100	0.06017986907116501	5.96636146469897
>500	0.0024601833070683247	0.9203412719417877
>1k	0.0015139589581958923	2.1161076506134973
>5k	3.784897395489731E-4	1.594928525934863
>10k+	3.784897395489731E-4	2.508927065129943
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT	12716	1.363689984664386	No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG	10622	1.1391251193067875	No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT	9085	0.9742940791660857	RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC	5751	0.616749064313061	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3716	0.39851148026209965	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3383	0.362799875599215	No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC	3027	0.32462170364730236	RNA PCR Primer, Index 41 (95% over 23bp)
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	2845	0.30510364944716717	No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC	2828	0.30328053449440734	No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG	1451	0.15560822332085752	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1285	0.13780604201743754	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1149	0.12322112239535857	No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTA	959	0.10284513174686584	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.0724205604469849E-4	0.0
5	0.0	0.0	0.0	1.0724205604469849E-4	0.0
6	0.0	0.0	0.0	1.0724205604469849E-4	0.0
7	0.0	0.0	0.0	1.0724205604469849E-4	0.0
8	0.0	0.0	0.0	1.0724205604469849E-4	0.0
9	0.0	0.0	0.0	1.0724205604469849E-4	0.0
10	0.0	0.0	0.0	2.1448411208939698E-4	0.0
11	0.0	0.0	0.0	2.1448411208939698E-4	0.0
12	0.0	0.0	0.0	2.1448411208939698E-4	0.0
13	0.0	0.0	0.0	3.2172616813409545E-4	0.0
14	0.0	0.0	0.0	3.2172616813409545E-4	0.0
15	0.0	0.0	0.0	3.2172616813409545E-4	0.0
16	0.0	0.0	0.0	4.2896822417879397E-4	0.0
17	0.0	0.0	0.0	5.362102802234925E-4	0.0
18	0.0	0.0	0.0	5.362102802234925E-4	0.0
19	0.0	0.0	0.0	5.362102802234925E-4	0.0
20	0.0	0.0	0.0	7.506943923128894E-4	0.0
21	0.0	0.0	0.0	0.0015013887846257788	0.0
22	0.0	0.0	0.0	0.0017158728967151759	0.0
23	0.0	0.0	0.0	0.0023593252329833668	0.0
24	0.0	0.0	0.0	0.0032172616813409545	0.0
25	0.0	0.0	0.0	0.0034317457934303517	0.0
26	0.0	0.0	0.0	0.003967956073653844	0.0
27	0.0	0.0	0.0	0.0047186504659667335	0.0
28	0.0	0.0	0.0	0.009544542987978166	0.0
29	0.0	0.0	0.0	0.0211266850408056	0.0
30	0.0	0.0	0.0	0.03903610840027025	0.0
31	0.0	0.0	0.0	0.08300535137859663	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1510	0.0	31.486755	1
CGTCTTC	460	0.0	29.76087	37
TTATACA	1620	0.0	29.691359	2
GGTATCA	675	0.0	27.68148	1
TATACAC	1780	0.0	27.02247	3
GCCGTCT	1410	0.0	25.585106	36
CCGTCTT	1405	0.0	25.544485	37
GTATCAA	2095	0.0	24.019093	1
GATTACG	60	3.725317E-5	21.583332	1
GTCTTAT	80	6.957216E-7	20.8125	1
TGCCGTC	1735	0.0	20.792505	35
TATATCG	55	5.142093E-4	20.181818	5
TTACGGG	55	5.142093E-4	20.181818	3
GCTTTAT	530	0.0	19.896227	1
ATGCCGT	1810	0.0	19.82873	34
ATTGAGC	520	0.0	19.567308	6
CCGTCTG	60	9.2350366E-4	18.5	27
TCTACAC	160	1.8189894E-12	18.5	3
TTAGTAC	90	2.1513297E-6	18.5	3
TATGCCG	1960	0.0	18.311224	33
>>END_MODULE