FastQCFastQC Report
Fri 10 Feb 2017
ERR1631044.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631044.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985296
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT173841.7643428979717772No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG122601.2442961302999302No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT121821.2363797275133566RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC62200.6312823760575502No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC51870.5264407853071564RNA PCR Primer, Index 33 (95% over 23bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC35220.35745603351683153No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21570.21891898475179034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19210.19496679170523376No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA13890.14097286500706385No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG13840.1404654032899758No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11710.11884753414202433No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTAT10410.1056535294977347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC20850.032.474821
TTACGGG800.032.3753
CGTCTTC6900.030.297137
TTATACA23450.029.1897642
TATACAC24600.027.9004083
GATTACG951.0913936E-1125.315791
CCGTCTT19300.024.1554437
GCCGTCT19950.023.46115536
GTATCAA11900.022.075631
GGTATCA5050.021.6138611
TAGGACG555.1423773E-420.1818184
CGGATCG851.2449382E-619.58823637
AGGACCG1052.2584572E-819.380955
TGCCGTC24200.019.26446235
TAGGACC1457.2759576E-1219.137934
TTAACGG609.2355366E-418.535
ATGCCGT25300.018.534
TACCGTT500.007034187718.4999989
TATGCCG26150.017.89866333
ATACACA40650.017.3849954
Produced by FastQC (version 0.11.5)