##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631044.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	985296
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53097140351732	31.0	31.0	34.0	28.0	34.0
2	31.54460081031487	31.0	31.0	34.0	30.0	34.0
3	31.00746374693493	31.0	31.0	34.0	27.0	34.0
4	35.122104423442295	37.0	35.0	37.0	32.0	37.0
5	35.33362664620581	37.0	35.0	37.0	33.0	37.0
6	35.528168184992126	37.0	35.0	37.0	33.0	37.0
7	35.54202493463893	37.0	35.0	37.0	33.0	37.0
8	35.564501429012196	37.0	35.0	37.0	33.0	37.0
9	37.351137120215654	39.0	37.0	39.0	34.0	39.0
10	37.18611056981861	39.0	37.0	39.0	33.0	39.0
11	37.24464120426755	39.0	37.0	39.0	34.0	39.0
12	37.182483233464865	39.0	37.0	39.0	33.0	39.0
13	37.25907443042497	39.0	37.0	39.0	34.0	39.0
14	38.42150683652425	40.0	38.0	41.0	34.0	41.0
15	38.43994089085919	40.0	38.0	41.0	34.0	41.0
16	38.429264911255096	40.0	38.0	41.0	34.0	41.0
17	38.39158486383787	40.0	38.0	41.0	34.0	41.0
18	38.42807339114337	40.0	38.0	41.0	34.0	41.0
19	38.44904373914032	40.0	38.0	41.0	34.0	41.0
20	38.44670434062454	40.0	38.0	41.0	34.0	41.0
21	38.410541603741414	40.0	38.0	41.0	34.0	41.0
22	38.34753414202432	40.0	38.0	41.0	34.0	41.0
23	38.345392653578216	40.0	38.0	41.0	34.0	41.0
24	38.29911518975008	40.0	38.0	41.0	34.0	41.0
25	38.257564224354915	40.0	38.0	41.0	34.0	41.0
26	38.16055073805232	40.0	38.0	41.0	33.0	41.0
27	38.0989885273055	40.0	37.0	41.0	33.0	41.0
28	38.09861300563485	40.0	38.0	41.0	33.0	41.0
29	38.01054200971079	40.0	37.0	41.0	33.0	41.0
30	37.943522555658404	40.0	37.0	41.0	33.0	41.0
31	37.94369610756564	40.0	37.0	41.0	33.0	41.0
32	37.84845670742599	40.0	37.0	41.0	33.0	41.0
33	37.8194400464429	40.0	37.0	41.0	33.0	41.0
34	37.75415814130982	40.0	37.0	41.0	33.0	41.0
35	37.740419122781375	40.0	37.0	41.0	33.0	41.0
36	37.71441881424465	40.0	37.0	41.0	33.0	41.0
37	37.66770391841639	40.0	37.0	41.0	33.0	41.0
38	37.64987171367792	40.0	37.0	41.0	33.0	41.0
39	37.57783752293727	40.0	37.0	41.0	32.0	41.0
40	37.49091745018756	40.0	37.0	41.0	32.0	41.0
41	37.458403363050294	40.0	37.0	41.0	32.0	41.0
42	37.400918099738554	40.0	37.0	41.0	32.0	41.0
43	36.75372882869716	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	10.0
10	13.0
11	11.0
12	16.0
13	16.0
14	9.0
15	20.0
16	8.0
17	17.0
18	25.0
19	33.0
20	70.0
21	130.0
22	311.0
23	596.0
24	1012.0
25	1747.0
26	2917.0
27	4710.0
28	7312.0
29	10591.0
30	14792.0
31	20221.0
32	26478.0
33	35156.0
34	46703.0
35	63866.0
36	89896.0
37	133990.0
38	237250.0
39	287362.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.308792484694955	21.78746285380231	14.764903135707442	25.138841525795293
2	13.831376560952242	21.630758675565513	41.041676815901006	23.496187947581234
3	14.738515126418864	27.839451291794546	34.03992302820675	23.38211055357984
4	11.822741592374271	19.246297559312126	37.78346811516539	31.147492733148212
5	12.961891654893554	37.560489436676896	35.73829590295708	13.739323005472468
6	28.245826634838668	39.76794790600997	17.732945226612102	14.253280232539256
7	24.92783894383008	32.983590717916236	22.776404248063525	19.312166090190157
8	24.602657475520047	35.71129893960799	21.87200597586918	17.814037609002778
9	24.067183871648723	15.202335135837352	19.833836735356687	40.89664425715724
10	14.483261882723566	28.708834705509815	33.550628440590444	23.257274971176177
11	31.742643834949092	22.975532225848884	25.400691771812735	19.881132167389293
12	20.820139328689045	25.90896542764814	29.586236014355077	23.68465922930774
13	28.70964664425716	20.860837758399505	26.644886409769246	23.784629187574087
14	21.66800636559978	21.94142673876683	26.574755200467674	29.815811695165717
15	23.874855880872346	28.632918432633442	22.707896916256637	24.784328770237572
16	24.910382260762248	27.377458144557576	23.807058995469383	23.905100599210797
17	21.190078920446243	30.275369026160664	25.188268297039674	23.34628375635342
18	23.815787337003297	26.392880921063316	25.801891005342558	23.98944073659083
19	23.322636040337116	28.862189636413827	25.470315519397218	22.34485880385184
20	23.01267842353973	27.061613971841965	25.55556908786801	24.3701385167503
21	25.086268491904974	26.507262792094966	25.420990240496256	22.985478475503808
22	22.14836962699534	30.89305142820026	25.589974992286578	21.368603952517823
23	25.087181922995725	25.544303437748656	25.696846429905328	23.671668209350287
24	24.58631720822981	28.21690131696465	25.139653464542633	22.057128010262904
25	22.406160179276075	28.646112437277733	25.437939461846998	23.509787921599195
26	24.50177408616294	29.01686396778227	25.219121969438625	21.262239976616165
27	21.772239002289666	30.69625777431351	25.742619476786672	21.788883746610153
28	23.478325295139733	26.112762053230703	26.833865153212844	23.57504749841672
29	22.63258959744077	29.219848654617497	25.715724005781006	22.43183774216073
30	23.04414104999919	27.692388886182428	26.968038031210927	22.295432032607458
31	25.90683408843637	26.048212922817104	25.31827998895763	22.726672999788896
32	22.657353729234668	27.818239392020267	26.661632646433155	22.862774232311914
33	23.526229681232845	27.173357041944758	27.57222195157597	21.728191325246424
34	23.635638427437033	27.578007015150778	26.31280346210682	22.473551095305368
35	21.97694905896299	25.780070151507772	29.10435036780825	23.138630421720986
36	23.325376333609395	27.74841265974895	25.246321917474546	23.679889089167112
37	22.27462610220685	26.893948620516067	27.402222276351473	23.42920300092561
38	22.521455481398483	25.28356960750881	27.89344521849272	24.301529692599992
39	24.00781085074942	25.639604748217792	26.561358211136554	23.791226189896232
40	21.643445218492715	25.38384404280541	30.630084766405226	22.342625972296652
41	22.6448701709943	25.787986554294346	26.405668956333933	25.16147431837742
42	23.0488096977964	25.27666812815641	29.031275880547568	22.64324629349962
43	23.11295285883633	24.743630340527112	29.04710868612072	23.09630811451584
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5.0
1	11.0
2	17.0
3	77.5
4	138.0
5	138.0
6	169.0
7	200.0
8	224.0
9	248.0
10	347.5
11	447.0
12	447.0
13	684.0
14	921.0
15	2180.5
16	3440.0
17	4048.0
18	4656.0
19	4656.0
20	4873.0
21	5090.0
22	6461.0
23	7832.0
24	10488.0
25	13144.0
26	13144.0
27	17209.0
28	21274.0
29	25558.0
30	29842.0
31	34631.5
32	39421.0
33	39421.0
34	45248.0
35	51075.0
36	55493.5
37	59912.0
38	64095.5
39	68279.0
40	68279.0
41	70070.5
42	71862.0
43	73082.0
44	74302.0
45	74882.5
46	75463.0
47	75463.0
48	75847.5
49	76232.0
50	86972.0
51	97712.0
52	79369.5
53	61027.0
54	61027.0
55	67004.5
56	72982.0
57	62447.5
58	51913.0
59	41753.0
60	31593.0
61	31593.0
62	27554.0
63	23515.0
64	19683.5
65	15852.0
66	13247.5
67	10643.0
68	10643.0
69	8699.0
70	6755.0
71	5500.5
72	4246.0
73	3283.5
74	2321.0
75	2321.0
76	1860.5
77	1400.0
78	1077.0
79	754.0
80	579.5
81	405.0
82	405.0
83	297.0
84	189.0
85	150.0
86	111.0
87	79.5
88	48.0
89	48.0
90	34.5
91	21.0
92	14.5
93	8.0
94	4.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	985296.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.981903622012595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.92653447337517	46.342926186767066
2	12.087219987753672	14.016800487759953
3	3.5543619738260537	6.182660202123861
4	1.4786637186877742	3.429429489052851
5	0.7687556698570126	2.228695857926252
6	0.4610968551042287	1.60411640478399
7	0.3109679359407042	1.262135902387507
8	0.21950855535374358	1.0182019120582382
9	0.15882513207729995	0.8288085150773484
>10	0.9265336495395727	9.936610894977711
>50	0.07075847538549576	2.7867079993916875
>100	0.03327132829900311	3.1694670993209195
>500	0.001400897920116782	0.5107130534851255
>1k	0.001225785680102184	1.27779796781517
>5k	3.502244800291955E-4	1.1581916105576195
>10k+	5.253367200437932E-4	4.246736416514684
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT	17384	1.7643428979717772	No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG	12260	1.2442961302999302	No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT	12182	1.2363797275133566	RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC	6220	0.6312823760575502	No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC	5187	0.5264407853071564	RNA PCR Primer, Index 33 (95% over 23bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC	3522	0.35745603351683153	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2157	0.21891898475179034	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1921	0.19496679170523376	No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA	1389	0.14097286500706385	No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG	1384	0.1404654032899758	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1171	0.11884753414202433	No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTAT	1041	0.1056535294977347	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.0149234341761258E-4	0.0
3	0.0	0.0	0.0	1.0149234341761258E-4	0.0
4	0.0	0.0	0.0	1.0149234341761258E-4	0.0
5	0.0	0.0	0.0	1.0149234341761258E-4	0.0
6	0.0	0.0	0.0	1.0149234341761258E-4	0.0
7	0.0	0.0	0.0	2.0298468683522516E-4	0.0
8	0.0	0.0	0.0	2.0298468683522516E-4	0.0
9	0.0	0.0	0.0	3.044770302528377E-4	0.0
10	0.0	0.0	0.0	8.119387473409006E-4	0.0
11	0.0	0.0	0.0	9.134310907585131E-4	0.0
12	0.0	0.0	0.0	9.134310907585131E-4	0.0
13	0.0	0.0	0.0	9.134310907585131E-4	0.0
14	0.0	0.0	0.0	0.0010149234341761256	0.0
15	0.0	0.0	0.0	0.0010149234341761256	0.0
16	0.0	0.0	0.0	0.0013194004644289635	0.0
17	0.0	0.0	0.0	0.0015223851512641887	0.0
18	0.0	0.0	0.0	0.0015223851512641887	0.0
19	0.0	0.0	0.0	0.0016238774946818013	0.0
20	0.0	0.0	0.0	0.0017253698380994137	0.0
21	0.0	0.0	0.0	0.002131339211769864	0.0
22	0.0	0.0	0.0	0.0024358162420227017	0.0
23	0.0	0.0	0.0	0.002841785615693152	0.0
24	0.0	0.0	0.0	0.0029432779591107646	0.0
25	0.0	0.0	0.0	0.0030447703025283774	0.0
26	0.0	0.0	0.0	0.00355223201961644	0.0
27	0.0	0.0	0.0	0.004364170766957341	0.0
28	0.0	0.0	0.0	0.00832237216024423	0.0
29	0.0	0.0	0.0	0.018573098845423103	0.0
30	0.0	0.0	0.0	0.02953427193452526	0.0
31	0.0	0.0	0.0	0.07246553320017537	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2085	0.0	32.47482	1
TTACGGG	80	0.0	32.375	3
CGTCTTC	690	0.0	30.2971	37
TTATACA	2345	0.0	29.189764	2
TATACAC	2460	0.0	27.900408	3
GATTACG	95	1.0913936E-11	25.31579	1
CCGTCTT	1930	0.0	24.15544	37
GCCGTCT	1995	0.0	23.461155	36
GTATCAA	1190	0.0	22.07563	1
GGTATCA	505	0.0	21.613861	1
TAGGACG	55	5.1423773E-4	20.181818	4
CGGATCG	85	1.2449382E-6	19.588236	37
AGGACCG	105	2.2584572E-8	19.38095	5
TGCCGTC	2420	0.0	19.264462	35
TAGGACC	145	7.2759576E-12	19.13793	4
TTAACGG	60	9.2355366E-4	18.5	35
ATGCCGT	2530	0.0	18.5	34
TACCGTT	50	0.0070341877	18.499998	9
TATGCCG	2615	0.0	17.898663	33
ATACACA	4065	0.0	17.384995	4
>>END_MODULE