FastQCFastQC Report
Fri 10 Feb 2017
ERR1631043.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631043.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349384
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT166134.75494012318824No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG165664.741487875804273No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT121283.4712522611224323TruSeq Adapter, Index 5 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC93202.6675520344377537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT49711.422789824376617No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48131.3775673757241318No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC44041.2605042016806722No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC44031.2602179836512262Illumina Paired End PCR Primer 2 (95% over 23bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA30870.8835550569001442No Hit
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG21860.6256726123691984No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA20340.5821674718933895No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT18190.5206305955624757No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17590.503457513795709No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA16540.4734046207038674No Hit
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTA15170.4341927506697502No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT14110.4038536395484625No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC13620.389828956105603No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT13370.3826735053694502No Hit
TATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTAT13230.3786664529572047No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG13090.3746594005449591No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC12200.34918599592425525No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG11090.317415794655737No Hit
ACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT10600.30339111121287754No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG9930.2842145032399881No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG9110.260744624825407No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG8990.25731000847205365No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8700.24900968561811643No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT8700.24900968561811643No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG8270.2367023103519337No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7840.22439493508575092No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG7670.21952922858516702No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG7600.21752570237904426No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7320.20951159755455318No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG7310.20922537952510703No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG7270.20808050740732262No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG7040.20149749273006204No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG6990.20006640258283148No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6770.19376960593501708No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6700.19176607972889428No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6620.1894763354933254No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC6450.1846106289927415No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT6290.18003114052160374No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6130.17545165205046595No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA6130.17545165205046595No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5970.1708721635793282No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5760.16486158496095984No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5560.15913722437203764No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA5280.15112311954754654No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG5250.15026446545920819No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG5240.1499782474297621No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC5130.14682984910585486No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5070.1451125409291782No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4910.14053305245804043No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4860.13910196231080987No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG4850.13881574428136376No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG4810.13767087216357934No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA4810.13767087216357934No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4780.136812218075241No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4720.13509490989856435No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4590.1313740755157649No Hit
ATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCT4430.12679458704462712No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4410.1262221509857349No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA4320.12364618872071989No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG4260.12192888054404323No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4220.12078400842625879No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA4220.12078400842625879No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4210.12049779039681267No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4190.11992535433792047No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA4180.11963913630847435No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4170.11935291827902823No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC4120.11792182813179769No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT4100.11734939207290544No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG4090.11706317404345935No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4070.11649073798456712No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA3900.11162503148398324No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA3860.11048015936619879No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3700.10590067089506101No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT3640.10418336271838435No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC3620.10361092665949213No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC3620.10361092665949213No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3620.10361092665949213No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA3610.10332470863004602No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3610.10332470863004602No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG3610.10332470863004602No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3550.10160740045336936No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGTG200.001840183537.01
AACGTGA200.001840183537.06
TTCTACA251.2318672E-437.02
ACACGAA200.001840183537.017
AATCATC251.2318672E-437.019
CGAAAGT200.001840183537.020
CGTGATC200.001840183537.08
TAAACGT200.001840183537.04
CACTGTG200.001840183537.07
AAAGTGG200.001840183537.022
TTATACC200.001840183537.030
GAAAGTG200.001840183537.021
TCTACAC308.295421E-637.03
AATTGCT308.295421E-637.034
CTCGGAG200.001840183537.01
ACGTGAT200.001840183537.07
GGACGTA200.001840183537.027
ACTGTGC308.295421E-637.08
CTTCTAC251.2318672E-437.01
ACTCAGA308.295421E-637.01
Produced by FastQC (version 0.11.5)