FastQCFastQC Report
Fri 10 Feb 2017
ERR1631042.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631042.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354139
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT176034.970647118786691No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG131743.72000824534999No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT118613.349249870813438TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC66751.8848531226439333No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT56281.589206498013492No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT55011.553344873058319No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC53291.5047763731190296RNA PCR Primer, Index 10 (95% over 22bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA40861.1537842485577698No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC35030.9891596237635504No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA24210.6836298741454626No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT22030.6220721242224099No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20860.5890342492637072No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT18930.5345358743318301No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT18470.5215466243480668No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA18060.5099692493625385No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC16890.47693137440383576No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG16790.4741076244073655No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG14730.41593837448007703No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC14710.4153736244807829No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG14540.4105732494867834No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA14300.40379624949525467No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG12130.3425208745718489No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT11500.32473124959408595No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG11430.32275462459655674No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT11040.3117419996103225No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG11000.31061249961173437No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG10180.28745774964067783No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC10130.28604587464244263No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG9770.27588037465514953No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG9550.26966812466291484No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA9500.2682562496646797No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG9390.26515012466856236No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG9330.26345587467068016No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG8750.24707812469115234No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG8430.23804212470244734No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT8270.23352412470809486No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG7820.22081724972397845No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC7780.21968774972539032No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA7710.2177111247278611No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT7450.2103693747370383No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7160.20218049974727437No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA6840.19314449975856937No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6830.19286212475892234No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT6300.1778962497776297No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA6120.17281349978398314No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG6060.17111924978610094No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG6010.16970737478786577No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG5930.16744837479068952No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC5910.16688362479139546No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5820.16434224979457218No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG5720.16151849979810187No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5690.16067137479916077No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA5660.1598242498002197No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5600.1581299998023375No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG5350.1510706248111617No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC5300.14965874981292657No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT5150.1454231248182211No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT5030.14203462482245674No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC4900.13836374982704533No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG4880.13779899982775126No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4850.13695187482881016No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4840.13666949982916313No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG4810.13582237483022203No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA4780.13497524983128092No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4770.13469287483163392No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA4740.13384574983269282No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4720.13328099983339875No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC4580.1293277498383403No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA4580.1293277498383403No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA4550.1284806248393992No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4530.12791587484010514No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA4450.1256568748429289No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4450.1256568748429289No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG4390.12396262484504672No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG4360.12311549984610562No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4350.1228331248464586No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4300.12142124984822343No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG4250.12000937484998829No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC4200.11859749985175312No Hit
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT4130.1166208748542239No Hit
TCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT4110.11605612485492983TruSeq Adapter, Index 10 (95% over 23bp)
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3970.11210287485987142No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3940.1112557498609303No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG3920.11069099986163625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC3810.1075848748655189No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA3660.10334924987081345No Hit
GTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT3590.10137262487328422No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTG3560.10052549987434312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACG308.2957395E-637.03
TGTCAAC308.2957395E-637.02
ACTCAGA403.8204234E-837.01
CCTTTAT200.001840216237.01
GATTACG800.037.01
TTGTGTT200.001840216237.03
TTACGGG750.037.03
CAACCTC251.2319013E-436.99999612
CTTATAC20100.035.711441
ATTACGG800.034.68752
TTATACA21200.033.7712252
GTTTGTG554.984031E-1033.6363641
CGTCTTC7500.033.337
GTGTCAA352.3836777E-531.7142851
TAGCAAC352.3836777E-531.7142851
AGCAACG352.3836777E-531.7142852
TTGTGTG352.3836777E-531.7142853
TATACAC22900.031.5065523
TCGACAT303.594494E-430.83333423
CACAACC303.594494E-430.83333410
Produced by FastQC (version 0.11.5)