FastQCFastQC Report
Fri 10 Feb 2017
ERR1631041.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631041.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484248
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT176053.63553385868398No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG144312.980084584758223No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT96922.0014538005319586No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT93121.922981612727363No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT92851.9174059572780888TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC78031.6113644248401646No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA47560.982141382101733No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC36320.7500289108060333No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT34070.7035651153954172No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC29440.6079529497282384RNA PCR Primer, Index 7 (95% over 22bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA28220.5827592473278155No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA28110.5804876839966299No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT26370.5445556822124201No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC21000.43366209049908305No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT20580.4249888486891015No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT20280.418793675967686No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG19670.40619682476747454No Hit
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG19240.39731707720011233No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC18280.3774925244915828No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG16890.3487882242156911No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG14440.2981943136574648No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG14210.2934446812377129No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG13920.28745601427367795No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13510.2789892782210768No Hit
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTA13420.2771307264046522No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC12620.2606102658142109No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT12580.25978424278468887No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG11990.2476004030992384No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG11880.24532883976805275No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG11850.24470932249591118No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG11670.2409922188630619No Hit
TATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTAT11450.23644909220069057No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG11390.23521005765640746No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG10630.21951562009548828No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA10600.21889610282334673No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG10510.21703755100692207No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG10250.21166840131502865No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA9820.20278865374766647No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA9660.19948456162957826No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG9490.19597396375410947No Hit
ACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT9180.18957228527531347No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC9100.18792023921626935No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC8840.18255108952437593No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG8800.18172506649485387No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8720.18007302043580975No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT8710.17986651467842923No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA8610.17780145710462408No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC8550.176562422560341No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8530.17614941104557993No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA8450.17449736498653584No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA8080.16685665196345673No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC8050.1662371346913152No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG7980.16479159438965157No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG7960.16437858287489054No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT7650.15797690439609455No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7610.1571508813665725No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG7320.15116221440253755No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA7300.1507492028877765No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA7270.15012968561563497No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA7210.14889065107135188No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7190.14847763955659082No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6920.14290198410731692No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG6890.14228246683517534No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC6830.14104343229089225No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG6760.13959789198922867No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG6610.13650030562852092No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA6450.13319621351043268No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT6280.12968561563496392No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG6220.1284465810906808No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA6200.12803356957591977No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT6200.12803356957591977No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA6130.12658802927425616No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG6070.12534899472997307No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC6070.12534899472997307No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC5920.12225140836926533No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT5890.1216318910971238No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC5880.12142538533974329No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC5820.12018635079546018No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA5680.11729527019213296No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA5630.11626274140523038No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA5570.11502370686094729No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT5540.11440418958880573No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA5490.11337166080190315No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA5390.111306603228098No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT5320.10986106292643438No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT5310.10965455716905387No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT5270.10882853413953181No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA5240.10820901686739028No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5160.10655697080834614No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG5060.10449191323454099No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG5010.10345938444763841No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA4920.10160083263121375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGTG308.302044E-637.01
TTACTTT600.037.03
TCTCAAC251.2325705E-437.03
ACTCAGA501.7826096E-1037.01
GATTACG800.037.01
GTTATCG200.001840884337.011
TTACGGG750.036.9999963
ATTACGG800.034.68752
CTTATAC19700.034.6522831
TGATACA507.3141564E-933.3000032
TAGTTCC507.3141564E-933.3000034
TTATACA20550.033.1289522
GGACGTA451.0777694E-732.8888927
CGTCTTC3400.032.6470637
GACAGAC352.3854745E-531.71428716
TTTACAC352.3854745E-531.7142872
CAGACAC352.3854745E-531.71428718
TATACAC21750.031.5563223
CTTAAAC303.596428E-430.8333343
GTCTCAA303.596428E-430.8333342
Produced by FastQC (version 0.11.5)