FastQCFastQC Report
Fri 10 Feb 2017
ERR1631040.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631040.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688953
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT204402.9668206684635963No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG171452.488558726066945No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT166182.412065844839924RNA PCR Primer, Index 39 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC86701.2584312717993826No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC84941.2328852621296373RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC52270.7586874576349911RNA PCR Primer, Index 39 (95% over 21bp)
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG19670.2855056876158461No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA18660.27084576161218543No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT15090.21902800336162262No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14780.21452842211297432No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13670.1984170182871691No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT11120.16140433382248134No Hit
TCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT8040.11669881690042716Illumina PCR Primer Index 11 (95% over 24bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC25350.034.445761
TTATACA27700.031.7906112
TATACAC29700.029.8989893
GCAGCGT250.00549483429.613
CGTCTTC9600.029.48437337
CCGTCTT27300.024.46336737
GCCGTCT28050.023.80926936
GGTATCA3600.022.6111131
GTATCAA7500.021.9533331
TCGGAAT555.14022E-420.18181826
TGCCGTC33500.019.9358235
ATGCCGT34500.019.35797134
ATACACA48400.018.6528934
CCTAATA801.6151396E-518.52
CAAGCGT500.00703209118.516
GCTCCGA500.00703209118.53
TAGTACG701.21829755E-418.54
TGTCCGA500.00703209118.51
TAATCGG500.00703209118.523
TATGCCG36550.018.42407833
Produced by FastQC (version 0.11.5)