##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631040.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	688953
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.227584465123165	31.0	31.0	34.0	28.0	34.0
2	31.264546347864076	31.0	31.0	34.0	28.0	34.0
3	30.700506420612147	31.0	31.0	34.0	26.0	34.0
4	34.86230991083572	37.0	35.0	37.0	32.0	37.0
5	35.04749235434057	37.0	35.0	37.0	32.0	37.0
6	35.25195042332351	37.0	35.0	37.0	32.0	37.0
7	35.25271244918013	37.0	35.0	37.0	32.0	37.0
8	35.29849496264622	37.0	35.0	37.0	32.0	37.0
9	37.05434042670545	39.0	37.0	39.0	33.0	39.0
10	36.850728569292826	39.0	37.0	39.0	33.0	39.0
11	36.91587524838414	39.0	37.0	39.0	33.0	39.0
12	36.83141230243572	39.0	37.0	39.0	33.0	39.0
13	36.93206648349017	39.0	37.0	39.0	33.0	39.0
14	37.98004653437898	40.0	37.0	41.0	33.0	41.0
15	37.99625228426322	40.0	37.0	41.0	33.0	41.0
16	37.97056257828908	40.0	37.0	41.0	33.0	41.0
17	37.90428664945214	40.0	37.0	41.0	33.0	41.0
18	37.98209747254167	40.0	37.0	41.0	33.0	41.0
19	37.98000734447778	40.0	37.0	41.0	33.0	41.0
20	38.009923753870005	40.0	37.0	41.0	33.0	41.0
21	37.98633578778233	40.0	37.0	41.0	33.0	41.0
22	37.88207178138422	40.0	37.0	41.0	33.0	41.0
23	37.918718693437725	40.0	37.0	41.0	33.0	41.0
24	37.82840774334389	40.0	37.0	41.0	33.0	41.0
25	37.77533590825499	39.0	37.0	41.0	33.0	41.0
26	37.66329778664147	39.0	37.0	41.0	32.0	41.0
27	37.551230635471505	39.0	37.0	41.0	32.0	41.0
28	37.602283464909796	39.0	37.0	41.0	32.0	41.0
29	37.5165635391674	39.0	37.0	41.0	32.0	41.0
30	37.457298248211416	39.0	37.0	40.0	32.0	41.0
31	37.46947759861703	39.0	37.0	40.0	32.0	41.0
32	37.338753151521225	39.0	36.0	40.0	32.0	41.0
33	37.299664853770864	39.0	36.0	40.0	31.0	41.0
34	37.225212750361784	39.0	36.0	40.0	31.0	41.0
35	37.22896627200985	39.0	36.0	40.0	31.0	41.0
36	37.20133013427621	39.0	36.0	40.0	31.0	41.0
37	37.15655784937434	39.0	36.0	40.0	31.0	41.0
38	37.1290000914431	39.0	36.0	40.0	31.0	41.0
39	37.00952169451327	39.0	36.0	40.0	31.0	41.0
40	36.88550162347794	39.0	36.0	40.0	30.0	41.0
41	36.86993887826891	39.0	36.0	40.0	30.0	41.0
42	36.81649111042408	39.0	36.0	40.0	30.0	41.0
43	36.08373575555952	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	16.0
10	26.0
11	22.0
12	20.0
13	15.0
14	17.0
15	15.0
16	15.0
17	18.0
18	29.0
19	45.0
20	92.0
21	187.0
22	310.0
23	650.0
24	992.0
25	1839.0
26	2941.0
27	4441.0
28	6657.0
29	9653.0
30	12974.0
31	17373.0
32	22637.0
33	29190.0
34	38244.0
35	51113.0
36	71554.0
37	104463.0
38	174957.0
39	138442.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.6367604176192	23.40928916776616	16.083680599402282	25.87026981521236
2	12.639323727453105	21.373301226643907	42.121305807507916	23.86606923839507
3	14.031000663325365	28.416597358600658	35.790540138441955	21.761861839632022
4	11.208602038165157	20.162333279628655	35.77123548340743	32.857829198798754
5	12.374719320476142	37.77050103562943	35.862968881766975	13.99181076212746
6	27.101558451737635	40.123782028672494	17.481744037691975	15.292915481897895
7	23.06630495839339	34.40075012373848	21.81222812006044	20.720716797807686
8	24.488898371877326	35.3082140581433	22.844664294951905	17.358223275027466
9	22.145487428024843	14.925546445113092	19.08243377995306	43.84653234690901
10	13.655793646300982	28.928533586471065	33.31330293938774	24.102369827840214
11	29.625097793318268	22.600235429702753	25.791164273905476	21.983502503073503
12	18.81899055523381	26.520096436186503	28.575389032343278	26.08552397623641
13	29.19502491461682	20.04374754155944	26.912430891512194	23.848796652311552
14	21.321483468393346	23.559662270140343	25.297081223247446	29.82177303821886
15	23.969559607113982	29.149738806565907	21.518739304422798	25.361962281897316
16	26.028045454479475	27.395192415157492	22.651037153477812	23.925724976885217
17	19.303784147830115	32.918646119546615	24.188152167128962	23.58941756549431
18	23.635429412456293	24.748132310912354	24.73840740950399	26.878030867127368
19	21.620487899755137	27.665602733423032	25.935150873862224	24.778758492959607
20	20.37076549488862	25.574748930623713	27.074560964245748	26.979924610241916
21	23.56183948687356	24.677880784320553	25.682448585026847	26.07783114377904
22	19.515845057645443	31.245817929524943	27.965623199260325	21.272713813569286
23	22.117909349404098	24.37350588501683	26.098877572200134	27.409707193378942
24	22.577156932330652	28.504556914622626	27.953575933336527	20.9647102197102
25	19.838944020854836	30.182174981457372	25.999015898036586	23.97986509965121
26	22.44362097269335	26.023691021013047	25.46080792158536	26.071880084708244
27	19.446028974400285	30.658695150467448	29.719298703975454	20.175977171156813
28	19.319750403873705	24.29106194471902	27.637589211455644	28.751598439951636
29	19.46112434375059	25.11985578116359	30.04268796274927	25.376331912336546
30	19.54443917074169	25.557331196757975	31.155100565640907	23.74312906685942
31	21.091279085801208	24.152881256050847	27.549629655433677	27.206210002714265
32	21.671870214659055	28.25272551248053	30.30714722194402	19.76825705091639
33	19.371132718777623	25.655741393099383	28.986737847139064	25.986388040983925
34	20.144480102416278	29.415068952453943	29.441921292163613	20.998529652966166
35	20.667883005081624	27.546581552007176	31.89433822045916	19.89119722245204
36	22.44986232732857	25.30288713453603	24.469884012407235	27.77736652572817
37	20.972693347731994	27.164552589218715	28.374504501758462	23.48824956129083
38	21.537463368328464	24.344766624138366	29.262228337782116	24.85554166975106
39	24.732166054868763	25.14844989425984	26.449844909594706	23.66953914127669
40	19.928209906916727	23.884938450082952	34.20305884436239	21.983792798637932
41	21.833564844045966	26.187127423786528	26.15258225161949	25.826725480548017
42	22.36814412594183	24.208763152203417	31.94209184080772	21.481000881047038
43	22.289619175763804	23.862295396057494	30.84738726734625	23.000698160832453
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13.0
1	13.5
2	14.0
3	65.0
4	116.0
5	116.0
6	150.0
7	184.0
8	209.0
9	234.0
10	309.0
11	384.0
12	384.0
13	616.5
14	849.0
15	1783.5
16	2718.0
17	3252.5
18	3787.0
19	3787.0
20	4315.0
21	4843.0
22	6419.0
23	7995.0
24	10568.0
25	13141.0
26	13141.0
27	16254.5
28	19368.0
29	21989.5
30	24611.0
31	28120.0
32	31629.0
33	31629.0
34	34412.5
35	37196.0
36	39171.0
37	41146.0
38	42659.5
39	44173.0
40	44173.0
41	45045.0
42	45917.0
43	45484.0
44	45051.0
45	44603.0
46	44155.0
47	44155.0
48	45430.5
49	46706.0
50	62257.0
51	77808.0
52	57537.5
53	37267.0
54	37267.0
55	48257.5
56	59248.0
57	50391.5
58	41535.0
59	30498.5
60	19462.0
61	19462.0
62	16825.5
63	14189.0
64	11867.5
65	9546.0
66	7989.0
67	6432.0
68	6432.0
69	5217.5
70	4003.0
71	3202.0
72	2401.0
73	1859.0
74	1317.0
75	1317.0
76	1021.0
77	725.0
78	571.0
79	417.0
80	312.0
81	207.0
82	207.0
83	151.5
84	96.0
85	69.5
86	43.0
87	28.5
88	14.0
89	14.0
90	11.5
91	9.0
92	5.5
93	2.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	688953.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.43865329850573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.50162557389434	44.51589001409522
2	13.868112724171125	15.931314373365222
3	4.2472233106017185	7.318643616569514
4	1.7252922404977162	3.96393851362202
5	0.8488015396772131	2.437700867837865
6	0.46845360246394374	1.6144406435017498
7	0.2988751091013316	1.2016888639857122
8	0.20188323565020963	0.9276720943434338
9	0.1372328179597212	0.7094221426768839
>10	0.6594245540959156	6.68568560993837
>50	0.03123590865739647	1.2407108312311808
>100	0.00806085666690326	0.731258129200663
>500	7.557053124954046E-4	0.293863328130577
>1k	0.0015114106249908092	1.345462869663336
>5k	7.557053124954046E-4	3.2397310586763908
>10k+	7.557053124954046E-4	7.842577043161823
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT	20440	2.9668206684635963	No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG	17145	2.488558726066945	No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	16618	2.412065844839924	RNA PCR Primer, Index 39 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC	8670	1.2584312717993826	No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC	8494	1.2328852621296373	RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC	5227	0.7586874576349911	RNA PCR Primer, Index 39 (95% over 21bp)
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG	1967	0.2855056876158461	No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA	1866	0.27084576161218543	No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT	1509	0.21902800336162262	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1478	0.21452842211297432	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1367	0.1984170182871691	No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT	1112	0.16140433382248134	No Hit
TCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT	804	0.11669881690042716	Illumina PCR Primer Index 11 (95% over 24bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.4514778221446164E-4	0.0
5	0.0	0.0	0.0	1.4514778221446164E-4	0.0
6	0.0	0.0	0.0	1.4514778221446164E-4	0.0
7	0.0	0.0	0.0	1.4514778221446164E-4	0.0
8	0.0	0.0	0.0	2.902955644289233E-4	0.0
9	0.0	0.0	0.0	7.257389110723083E-4	0.0
10	0.0	0.0	0.0	0.0010160344755012316	0.0
11	0.0	0.0	0.0	0.0010160344755012316	0.0
12	0.0	0.0	0.0	0.0010160344755012316	0.0
13	0.0	0.0	0.0	0.0010160344755012316	0.0
14	0.0	0.0	0.0	0.0010160344755012316	0.0
15	0.0	0.0	0.0	0.0010160344755012316	0.0
16	0.0	0.0	0.0	0.0010160344755012316	0.0
17	0.0	0.0	0.0	0.0010160344755012316	0.0
18	0.0	0.0	0.0	0.0011611822577156932	0.0
19	0.0	0.0	0.0	0.0011611822577156932	0.0
20	0.0	0.0	0.0	0.0015966256043590781	0.0
21	0.0	0.0	0.0	0.00174177338657354	0.0
22	0.0	0.0	0.0	0.00174177338657354	0.0
23	0.0	0.0	0.0	0.0018869211687880015	0.0
24	0.0	0.0	0.0	0.0024675122976458483	0.0
25	0.0	0.0	0.0	0.00261266007986031	0.0
26	0.0	0.0	0.0	0.00261266007986031	0.0
27	0.0	0.0	0.0	0.00348354677314708	0.0
28	0.0	0.0	0.0	0.008128275804009853	0.0
29	0.0	0.0	0.0	0.01770802943016432	0.0
30	0.0	0.0	0.0	0.03164221652275264	0.0
31	0.0	0.0	0.0	0.06676797981865236	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2535	0.0	34.44576	1
TTATACA	2770	0.0	31.790611	2
TATACAC	2970	0.0	29.898989	3
GCAGCGT	25	0.005494834	29.6	13
CGTCTTC	960	0.0	29.484373	37
CCGTCTT	2730	0.0	24.463367	37
GCCGTCT	2805	0.0	23.809269	36
GGTATCA	360	0.0	22.611113	1
GTATCAA	750	0.0	21.953333	1
TCGGAAT	55	5.14022E-4	20.181818	26
TGCCGTC	3350	0.0	19.93582	35
ATGCCGT	3450	0.0	19.357971	34
ATACACA	4840	0.0	18.652893	4
CCTAATA	80	1.6151396E-5	18.5	2
CAAGCGT	50	0.007032091	18.5	16
GCTCCGA	50	0.007032091	18.5	3
TAGTACG	70	1.21829755E-4	18.5	4
TGTCCGA	50	0.007032091	18.5	1
TAATCGG	50	0.007032091	18.5	23
TATGCCG	3655	0.0	18.424078	33
>>END_MODULE