FastQCFastQC Report
Fri 10 Feb 2017
ERR1631039.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631039.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423901
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG195384.609095048136239No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT139253.2849651215731974RNA PCR Primer, Index 47 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT133483.1488484339503797No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC107772.5423388951665604No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC51581.2167935437755513No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC50181.1837669644563236RNA PCR Primer, Index 47 (95% over 23bp)
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG25600.6039145932658805No Hit
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA16710.3941958145887837No Hit
TATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTAT15890.374851675273236No Hit
ACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT12000.2830849655933815No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9930.23425280902852316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9510.2243448352327548No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA6960.16418928004416125No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC6260.14767599038454732No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC4980.11748026072125331No Hit
ATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCT4870.11488531520331398No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA4810.11346989037534706No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA4620.10898771175345187No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC4530.10686457451150151No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT4300.10143877933762836No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGCA200.001840625837.09
CTTATAC15650.034.399361
TTATACA17250.031.315942
CGTCTTC6850.031.05839337
TTAGTAC250.00549247829.63
TATACAC19000.028.918423
GGTATCA2300.027.3478261
TCCGTCA358.8601245E-426.4285725
TCGCCGA509.073421E-625.9000021
CCGTCTT20700.024.66666837
GCCGTCT21250.024.02823436
ACAGCCG400.001929187623.12536
CTTAGTA400.001929187623.1252
ACGTGGG1400.022.46428536
GATACAC753.7292557E-722.23
GCCGAGC603.7191494E-521.5833323
CGCCGAG603.7191494E-521.5833322
CGTGGGA1400.021.14285737
CCGTCAT450.003821884320.55555726
GCCACGT1550.020.29032333
Produced by FastQC (version 0.11.5)