##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631039.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	423901
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.604200037272854	31.0	30.0	33.0	27.0	34.0
2	30.65351107923784	31.0	30.0	34.0	27.0	34.0
3	30.079391178600662	31.0	30.0	34.0	25.0	34.0
4	34.29506417772074	35.0	35.0	37.0	30.0	37.0
5	34.25615414919993	35.0	35.0	37.0	30.0	37.0
6	34.50467915857712	35.0	35.0	37.0	30.0	37.0
7	34.54560852651916	35.0	35.0	37.0	30.0	37.0
8	34.60700965555637	35.0	35.0	37.0	31.0	37.0
9	36.27173797655585	37.0	35.0	39.0	32.0	39.0
10	36.01043639906488	37.0	35.0	39.0	31.0	39.0
11	36.07811729625549	37.0	35.0	39.0	32.0	39.0
12	35.97862000797356	37.0	35.0	39.0	31.0	39.0
13	36.12440404717139	37.0	35.0	39.0	32.0	39.0
14	36.96434073050075	38.0	36.0	40.0	31.0	41.0
15	37.00175984486944	38.0	36.0	40.0	31.0	41.0
16	36.96512865032165	38.0	36.0	40.0	31.0	41.0
17	36.88943880764612	38.0	36.0	40.0	31.0	41.0
18	37.0033168121802	38.0	36.0	40.0	31.0	41.0
19	36.97165611781996	39.0	36.0	40.0	31.0	41.0
20	37.07011542789472	39.0	36.0	40.0	31.0	41.0
21	36.97524893784162	39.0	36.0	40.0	31.0	41.0
22	36.765294255026525	38.0	36.0	40.0	31.0	41.0
23	36.884756110506935	39.0	36.0	40.0	31.0	41.0
24	36.67531805775405	38.0	35.0	40.0	30.0	41.0
25	36.69295425111052	38.0	35.0	40.0	31.0	41.0
26	36.51484662692468	38.0	35.0	40.0	30.0	41.0
27	36.370138310596104	38.0	35.0	40.0	30.0	41.0
28	36.45764930962654	38.0	35.0	40.0	30.0	41.0
29	36.28355441482799	38.0	35.0	40.0	30.0	41.0
30	36.2933515136789	38.0	35.0	40.0	30.0	41.0
31	36.279421374330326	38.0	35.0	40.0	30.0	41.0
32	36.055055307725155	38.0	35.0	40.0	30.0	41.0
33	36.05431220969047	38.0	35.0	40.0	30.0	41.0
34	35.944852689661026	38.0	35.0	40.0	29.0	41.0
35	35.99570418564712	38.0	35.0	40.0	30.0	41.0
36	36.045951767039945	38.0	35.0	40.0	29.0	41.0
37	35.93465455377553	38.0	34.0	40.0	29.0	41.0
38	35.876265920580515	38.0	34.0	40.0	29.0	41.0
39	35.803435236057474	38.0	34.0	40.0	28.0	41.0
40	35.57224918082288	38.0	34.0	40.0	27.0	41.0
41	35.61751446682126	38.0	34.0	40.0	28.0	41.0
42	35.582930920191274	38.0	34.0	40.0	28.0	41.0
43	34.6723598198636	37.0	33.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	5.0
10	12.0
11	18.0
12	18.0
13	14.0
14	16.0
15	13.0
16	13.0
17	12.0
18	38.0
19	49.0
20	108.0
21	211.0
22	410.0
23	713.0
24	1178.0
25	1962.0
26	3078.0
27	4388.0
28	6462.0
29	9101.0
30	11896.0
31	15796.0
32	20495.0
33	26186.0
34	33561.0
35	44573.0
36	58846.0
37	74482.0
38	78402.0
39	31843.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.61964704022873	26.918077569998655	17.143389612197186	24.318885777575424
2	12.393459793678241	22.454535375005015	41.991644275432236	23.16036055588451
3	14.086779696202651	31.11080181457463	36.48068770774308	18.32173078147964
4	10.893109476033318	20.697757259360085	33.16316781512664	35.24596544947995
5	10.819035576703051	40.61632315092439	34.78335743487276	13.781283837499794
6	25.090056404679395	39.34432803885813	17.752258192360955	17.813357364101524
7	20.24718035579062	37.01383105961062	20.76900030903442	21.969988275564344
8	23.929643949884525	34.34811430027294	24.99781788672355	16.724423863118982
9	20.399102620659068	15.221478599956123	17.87540015239407	46.504018626990735
10	13.3960523801548	28.410643051089757	33.61280110214413	24.580503466611308
11	28.263674773119195	22.221462086666463	25.15257100124793	24.36229213896641
12	17.10894760804999	28.498163486285712	26.62296149336755	27.76992741229674
13	30.408987004041037	19.31512310657441	26.375733956749336	23.900155932635215
14	20.685962052460365	25.847544591779688	23.85533414641626	29.61115920934369
15	24.731718019065774	29.273108579597597	20.6031597000243	25.392013701312333
16	26.305198619488984	27.14195059695542	20.924461135972784	25.62838964758281
17	18.189624464202726	35.1112641866851	22.326439428074007	24.372671921038165
18	22.8742088364972	25.131811437104417	23.109169357939706	28.884810368458673
19	21.731725096189912	33.154911170296835	24.76568821493698	20.34767551857627
20	20.605518741404243	25.94733204215135	26.760257701680345	26.68689151476406
21	28.62484400838875	25.840939275915837	22.694449883345406	22.839766832350005
22	18.770892260221135	35.25870427293165	24.01362582301056	21.95677764383665
23	27.34341273080271	24.456181985888215	23.354745565591966	24.84565971771711
24	22.25967855702157	32.69324677224163	23.08888160207218	21.958193068664617
25	21.626275946506375	30.485419944751253	25.690196531737364	22.198107577005008
26	27.47740628118358	29.8204061797448	22.810042910962704	19.89214462810892
27	18.18396276489086	31.9631234651487	28.967848625032733	20.885065144927704
28	25.74634171658005	24.643489871455834	27.057496915553397	22.552671496410717
29	18.32432572699758	29.058671718160607	24.516809349352798	28.100193205489017
30	23.327852493860597	26.774883758236	31.00983484351299	18.88742890439041
31	26.044288642867087	23.603860335314142	23.36866390973364	26.983187112085133
32	21.384710109200025	30.911462817969287	26.075427989082357	21.628399083748327
33	26.107275047711614	24.462551397614067	29.898018641144986	19.532154913529336
34	19.008211823043588	31.759774098197457	24.841413443233208	24.390600635525747
35	19.532626721805325	27.537325932234175	35.035774862526864	17.89427248343363
36	25.40027034614214	25.162950783319687	23.078030011724433	26.35874885881373
37	21.04194139669404	26.527420317479788	29.884336201141302	22.546302084684868
38	20.380938002033496	23.740920639488937	29.812621343190983	26.065520015286587
39	26.328081320874446	25.395552263382253	25.68264759932154	22.59371881642176
40	18.779384809188937	22.544178947442916	37.6477054784018	21.02873076496635
41	21.9204484065855	27.612815256392416	25.221926817818314	25.244809519203777
42	21.549135293382182	22.947339119275494	34.228982710585726	21.274542876756602
43	22.819243172344486	23.216505740727197	30.970202948329916	22.9940481385984
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10.0
1	10.5
2	11.0
3	28.5
4	46.0
5	46.0
6	68.0
7	90.0
8	93.0
9	96.0
10	174.5
11	253.0
12	253.0
13	377.5
14	502.0
15	1271.0
16	2040.0
17	2336.5
18	2633.0
19	2633.0
20	2948.5
21	3264.0
22	4374.0
23	5484.0
24	7016.0
25	8548.0
26	8548.0
27	10368.0
28	12188.0
29	15295.5
30	18403.0
31	19403.5
32	20404.0
33	20404.0
34	21597.0
35	22790.0
36	23524.0
37	24258.0
38	25328.0
39	26398.0
40	26398.0
41	27526.0
42	28654.0
43	26335.0
44	24016.0
45	23884.0
46	23752.0
47	23752.0
48	22930.5
49	22109.0
50	24150.5
51	26192.0
52	41315.5
53	56439.0
54	56439.0
55	37102.5
56	17766.0
57	28273.5
58	38781.0
59	30466.5
60	22152.0
61	22152.0
62	14297.0
63	6442.0
64	5272.0
65	4102.0
66	3314.0
67	2526.0
68	2526.0
69	1976.5
70	1427.0
71	1188.5
72	950.0
73	767.5
74	585.0
75	585.0
76	450.5
77	316.0
78	231.0
79	146.0
80	109.5
81	73.0
82	73.0
83	51.0
84	29.0
85	25.0
86	21.0
87	13.0
88	5.0
89	5.0
90	2.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	423901.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.22324678927093
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.47840924815308	35.038252222693586
2	13.06776406238285	11.819334383542108
3	4.168791709451346	5.655788888687547
4	1.8097279893302392	3.2736710193172986
5	0.9066584552471175	2.0501019537609775
6	0.5515089367224466	1.4964614851132567
7	0.3232989715478164	1.0234440423917068
8	0.22889487576471762	0.8281095564405869
9	0.15775804863900547	0.6420898049935998
>10	1.0975229390326968	9.690316116702997
>50	0.1267376556867302	3.991993380633855
>100	0.07562535010113461	6.081274170332761
>500	0.002086216554514058	0.7703308940118215
>1k	0.002086216554514058	1.6557632810664382
>5k	0.001043108277257029	2.4001491664005807
>10k+	0.002086216554514058	13.582919633910834
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG	19538	4.609095048136239	No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT	13925	3.2849651215731974	RNA PCR Primer, Index 47 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT	13348	3.1488484339503797	No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC	10777	2.5423388951665604	No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC	5158	1.2167935437755513	No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC	5018	1.1837669644563236	RNA PCR Primer, Index 47 (95% over 23bp)
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG	2560	0.6039145932658805	No Hit
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA	1671	0.3941958145887837	No Hit
TATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTAT	1589	0.374851675273236	No Hit
ACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT	1200	0.2830849655933815	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	993	0.23425280902852316	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	951	0.2243448352327548	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	696	0.16418928004416125	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	626	0.14767599038454732	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	498	0.11748026072125331	No Hit
ATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCT	487	0.11488531520331398	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	481	0.11346989037534706	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	462	0.10898771175345187	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	453	0.10686457451150151	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	430	0.10143877933762836	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	2.3590413799448457E-4	0.0
16	0.0	0.0	0.0	2.3590413799448457E-4	0.0
17	0.0	0.0	0.0	4.7180827598896914E-4	0.0
18	0.0	0.0	0.0	4.7180827598896914E-4	0.0
19	0.0	0.0	0.0	4.7180827598896914E-4	0.0
20	0.0	0.0	0.0	4.7180827598896914E-4	0.0
21	0.0	0.0	0.0	9.436165519779383E-4	0.0
22	0.0	0.0	0.0	0.0011795206899724228	0.0
23	0.0	0.0	0.0	0.0014154248279669075	0.0
24	0.0	0.0	0.0	0.0014154248279669075	0.0
25	0.0	0.0	0.0	0.0014154248279669075	0.0
26	0.0	0.0	0.0	0.001651328965961392	0.0
27	0.0	0.0	0.0	0.002123137241950361	0.0
28	0.0	0.0	0.0	0.004718082759889691	0.0
29	0.0	0.0	0.0	0.012738823451702167	0.0
30	0.0	0.0	0.0	0.026185359317387788	0.0
31	0.0	0.0	0.0	0.05897603449862114	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGAGCA	20	0.0018406258	37.0	9
CTTATAC	1565	0.0	34.39936	1
TTATACA	1725	0.0	31.31594	2
CGTCTTC	685	0.0	31.058393	37
TTAGTAC	25	0.005492478	29.6	3
TATACAC	1900	0.0	28.91842	3
GGTATCA	230	0.0	27.347826	1
TCCGTCA	35	8.8601245E-4	26.42857	25
TCGCCGA	50	9.073421E-6	25.900002	1
CCGTCTT	2070	0.0	24.666668	37
GCCGTCT	2125	0.0	24.028234	36
ACAGCCG	40	0.0019291876	23.125	36
CTTAGTA	40	0.0019291876	23.125	2
ACGTGGG	140	0.0	22.464285	36
GATACAC	75	3.7292557E-7	22.2	3
GCCGAGC	60	3.7191494E-5	21.583332	3
CGCCGAG	60	3.7191494E-5	21.583332	2
CGTGGGA	140	0.0	21.142857	37
CCGTCAT	45	0.0038218843	20.555557	26
GCCACGT	155	0.0	20.290323	33
>>END_MODULE