FastQCFastQC Report
Fri 10 Feb 2017
ERR1631038.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631038.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277284
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT154195.560724744305477No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG133954.8307872073397675No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT114934.144847881594322TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC68272.4620966229569685No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC50601.8248438424142759RNA PCR Primer, Index 1 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT47381.7087174160788217No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46721.6849151050908093No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC37221.3423060832936629No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA27250.9827469309444469No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19070.6877425311233248No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA15230.5492563581021623No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA14880.5366339204570043No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG14520.5236508417362704No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT14470.5218476363583907No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC11720.4226713405750061No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT11480.4140159547611834No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT11390.410770185081No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT11350.4093276207786962No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11060.39886902958699383No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG10780.3887710794708674No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC10220.36857517923861455No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG9510.34296966287272257No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT8830.3184460697335584No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG8040.2899554247630588No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG8000.288512860460755No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG6910.24920298322297718No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6860.24739977784509745No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG6770.24415400816491395No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6710.2419901617114583No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6440.2322528526709078No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6190.2232368257815092No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG6010.21674528642114224No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5990.21602400426999036No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5860.21133567028750305No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5750.20736861845616766No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5710.20592605415386392No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5650.20376220770040826No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG5630.20304092554925637No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4960.1788779734856681No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4910.1770747681077884No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4860.17527156272990868No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4830.17418963950318087No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4670.16841938229396575No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4470.16120656078244686No Hit
ATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT4290.15471502142207988No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA4270.153993739270928No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA4230.15255117496862425No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC3970.1431745070036497No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3920.14137130162576997No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3870.13956809624789024No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3760.13560104441655488No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3740.134879762265403No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG3670.13235527473637138No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG3580.12910950505618787No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3580.12910950505618787No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3560.128388222905036No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3490.12586373537600437No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA3430.12369988892254873No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3380.12189668354466901No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3330.12009347816678928No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3330.12009347816678928No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT3290.11865091386448551No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3270.11792963171333361No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3260.11756899063775769No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG3210.11576578525987796No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC3170.11432322095757419No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC3140.11324129773084635No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG3140.11324129773084635No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA3030.10927424589951097No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3030.10927424589951097No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3020.10891360482393503No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC2920.10530719406817558No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA2910.10494655299259964No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA2910.10494655299259964No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT2900.1045859119170237No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC2830.1020614243879921No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG2820.10170078331241615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGA452.6047928E-937.01
TGATACA308.289238E-637.02
TAACGCA200.001839528637.04
TTACGGG403.8153303E-837.03
ACTCAGA403.8153303E-837.01
GATTACG403.8153303E-837.01
TTTACAC251.2312109E-436.9999962
CTTATAC19250.035.4623371
CGTCTTC6000.035.45833237
GCCGAGC507.2905095E-933.33
TTATACA20550.033.2189752
GATACAC352.3818235E-531.7142873
TATACAC21950.031.3530773
CGCCGAG551.845001E-830.2727282
AGCAACG250.005489242329.5999982
GAATGGT250.005489242329.59999819
ATTACGG502.7082024E-729.5999982
TAGCAAC250.005489242329.5999981
TCAGAGT502.7082024E-729.5999983
GGTATCA6100.026.6885221
Produced by FastQC (version 0.11.5)