FastQCFastQC Report
Fri 10 Feb 2017
ERR1631037.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631037.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902885
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT150061.662005681786717No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG129441.433626652342214No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT88650.9818526168891941Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC68000.7531413192156254No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC33100.3666026127358412No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28590.3166516222996284No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC28260.31299667177990553Illumina PCR Primer Index 11 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26120.28929487143988436No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA18610.20611705809709985No Hit
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG16800.1860702082768016No Hit
TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTA11970.13257502339722113No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10600.11740144093655339No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9910.10975927166804189No Hit
TATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTAT9650.10687961368280569No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9600.10632583330102947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC17100.033.2134481
TTATACA18750.029.6986682
CGTCTTC3750.029.10666537
TATACAC19750.028.9443023
CCGTCTT11900.026.11764737
GCCGTCT12150.025.58024836
GTATCAA15550.024.9839231
GGTATCA5550.024.01
CGGATCG502.7014807E-422.19999937
CCGCGTG502.7014807E-422.19999937
TCTATAC1005.347829E-1022.1999993
GCCGCGT603.7251477E-521.58333236
AATTCCG450.003825182620.55555514
ACTCACG450.003825182620.55555529
GCGAGGT450.003825182620.55555521
CGGTGTA555.1419187E-420.18181834
AGGGACG656.9000336E-519.92307732
TAAACCG759.262161E-619.7333345
TCGTCTT951.6751255E-719.47368414
GATTACG1254.110916E-1019.2400021
Produced by FastQC (version 0.11.5)