##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631037.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	902885
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50412178738156	31.0	31.0	34.0	28.0	34.0
2	31.517153347325518	31.0	31.0	34.0	30.0	34.0
3	30.95943115679184	31.0	31.0	34.0	27.0	34.0
4	35.08409044341195	37.0	35.0	37.0	32.0	37.0
5	35.31172740714487	37.0	35.0	37.0	33.0	37.0
6	35.50895961279676	37.0	35.0	37.0	33.0	37.0
7	35.52525626187167	37.0	35.0	37.0	33.0	37.0
8	35.540782048655146	37.0	35.0	37.0	33.0	37.0
9	37.3228639306224	39.0	37.0	39.0	34.0	39.0
10	37.16154106004641	39.0	37.0	39.0	33.0	39.0
11	37.21453784258239	39.0	37.0	39.0	33.0	39.0
12	37.147020938436235	39.0	37.0	39.0	33.0	39.0
13	37.219403357016674	39.0	37.0	39.0	34.0	39.0
14	38.36791285711912	40.0	38.0	41.0	34.0	41.0
15	38.376658156908135	40.0	38.0	41.0	34.0	41.0
16	38.37627051064089	40.0	38.0	41.0	34.0	41.0
17	38.35697569457904	40.0	38.0	41.0	34.0	41.0
18	38.38857551072396	40.0	38.0	41.0	34.0	41.0
19	38.42409276928956	40.0	38.0	41.0	34.0	41.0
20	38.42297081023607	40.0	38.0	41.0	34.0	41.0
21	38.38545551205303	40.0	38.0	41.0	34.0	41.0
22	38.317259673158816	40.0	38.0	41.0	34.0	41.0
23	38.32862324659287	40.0	38.0	41.0	34.0	41.0
24	38.28399408562552	40.0	38.0	41.0	34.0	41.0
25	38.242150439978516	40.0	38.0	41.0	34.0	41.0
26	38.155164832730634	40.0	38.0	41.0	33.0	41.0
27	38.07934676066166	40.0	37.0	41.0	33.0	41.0
28	38.08917746999895	40.0	37.0	41.0	33.0	41.0
29	38.01769328319775	40.0	37.0	41.0	33.0	41.0
30	37.916962846874185	40.0	37.0	41.0	33.0	41.0
31	37.904540445350186	40.0	37.0	41.0	33.0	41.0
32	37.791365456287345	40.0	37.0	41.0	33.0	41.0
33	37.775247124495365	40.0	37.0	41.0	33.0	41.0
34	37.71885899090139	40.0	37.0	41.0	33.0	41.0
35	37.67959263915117	40.0	37.0	41.0	33.0	41.0
36	37.64794409033266	40.0	37.0	41.0	33.0	41.0
37	37.598608903680976	40.0	37.0	41.0	33.0	41.0
38	37.5654795461216	40.0	37.0	41.0	32.0	41.0
39	37.48252656761382	40.0	37.0	41.0	32.0	41.0
40	37.38280844182814	39.0	37.0	41.0	32.0	41.0
41	37.336769356008794	39.0	36.0	41.0	31.0	41.0
42	37.26809505086473	39.0	36.0	41.0	31.0	41.0
43	36.62010665810153	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	5.0
10	21.0
11	12.0
12	9.0
13	9.0
14	10.0
15	10.0
16	15.0
17	11.0
18	14.0
19	36.0
20	66.0
21	143.0
22	264.0
23	499.0
24	983.0
25	1797.0
26	2911.0
27	4551.0
28	7010.0
29	10121.0
30	14098.0
31	19046.0
32	24983.0
33	32827.0
34	43698.0
35	58947.0
36	82221.0
37	123687.0
38	215854.0
39	259022.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.371996433654346	22.206371797072716	14.980756131733278	24.440875637539662
2	13.882498878594726	22.07346450544643	40.968451131650205	23.075585484308633
3	14.600198253376675	28.60020932898431	33.9263582848314	22.87323413280761
4	11.746789458236652	19.378326143418043	37.713994584027866	31.16088981431744
5	12.696412056906473	38.1934576385697	35.495882642861496	13.614247661662338
6	27.883839027118622	39.64436223882333	17.92376659264469	14.548032141413358
7	24.525493279875064	33.089485371891215	22.907347004325025	19.477674343908692
8	24.48473504377634	35.29552490073486	22.309817972388508	17.909922083100284
9	24.002835355554694	15.119644251482747	19.793772185826544	41.08374820713601
10	14.433399602385686	28.939455190860407	33.478128443821745	23.149016762932156
11	31.91879364481634	22.984876257773692	25.235328973235795	19.861001124174173
12	20.929354236696813	26.098672588424883	29.77322693366265	23.19874624121566
13	29.11666491302879	21.022721609064277	26.511571241077213	23.349042236829717
14	21.662337950015782	22.24292130226995	26.596742663794394	29.497998083919878
15	24.02941681387995	28.587583136279815	22.884309740443136	24.4986903093971
16	24.360798994334825	27.365500589776104	23.90614530089657	24.367555114992495
17	21.088842986648356	29.957082020412347	25.303333204117912	23.65074178882139
18	23.86062455351457	25.58675800351097	26.605160125597386	23.947457317377076
19	23.550064515414476	27.54658677461692	26.87739856127857	22.025950148690033
20	22.795040342900812	26.828998155911325	25.764078481755703	24.611883019432153
21	24.508769112345426	25.67702420574049	26.22205485748462	23.592151824429468
22	22.470414283103608	29.80002990414062	25.84249378381521	21.887062028940562
23	23.16241824817114	25.37443860513797	26.71215049535655	24.750992651334332
24	23.840134679388846	27.29140477469445	27.190284476982118	21.678176068934583
25	22.93182409719953	28.006446003643877	26.070762057183362	22.99096784197323
26	23.703572437242837	25.91149481938453	27.505828538518195	22.87910420485444
27	22.672765634604627	29.529342053528413	26.373126145633165	21.424766166233795
28	21.166372240097022	25.532376770020544	27.180094917957437	26.121156071924993
29	23.63578971851343	25.935085863648194	28.282782414150198	22.146342003688176
30	22.281353660765213	27.10910027301373	27.45222259756226	23.1573234686588
31	22.832254384556173	24.969403633906865	27.185521965698843	25.012820015838116
32	25.094114975882864	26.133449996400426	27.0082014874541	21.764233540262605
33	21.37880239454637	25.61466853475249	28.115097714548366	24.891431356152776
34	21.734993936104818	27.353871201758807	28.374045421066914	22.53708944106946
35	23.21713174989063	25.756214800334483	29.753733864224124	21.272919585550763
36	23.22986869867148	25.747575826378778	25.637041262176247	25.385514212773497
37	22.376936154659784	27.058152477890317	27.822701672970535	22.742209694479364
38	22.260642274486784	25.387286309995183	28.10092093677489	24.25115047874314
39	23.885101646389074	25.571584421050296	26.934548696677872	23.608765235882753
40	21.63099398040725	25.3147410799825	30.865946382983438	22.18831855662681
41	22.832365140632525	25.99821682717068	26.33834873765762	24.83106929453917
42	23.006252180510252	25.19357393244987	29.10891198768392	22.69126189935595
43	23.36897833057366	24.849011778908718	28.949533993808735	22.832475896708885
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5.0
1	12.5
2	20.0
3	67.0
4	114.0
5	114.0
6	162.5
7	211.0
8	219.5
9	228.0
10	357.0
11	486.0
12	486.0
13	797.0
14	1108.0
15	2663.5
16	4219.0
17	4866.5
18	5514.0
19	5514.0
20	5625.0
21	5736.0
22	6698.0
23	7660.0
24	10191.5
25	12723.0
26	12723.0
27	16307.0
28	19891.0
29	23941.5
30	27992.0
31	32387.5
32	36783.0
33	36783.0
34	40915.0
35	45047.0
36	48161.5
37	51276.0
38	55304.0
39	59332.0
40	59332.0
41	62717.0
42	66102.0
43	67366.5
44	68631.0
45	69190.5
46	69750.0
47	69750.0
48	69008.5
49	68267.0
50	66921.5
51	65576.0
52	76316.0
53	87056.0
54	87056.0
55	69372.0
56	51688.0
57	53642.0
58	55596.0
59	46094.0
60	36592.0
61	36592.0
62	28796.5
63	21001.0
64	17523.5
65	14046.0
66	11257.5
67	8469.0
68	8469.0
69	6912.5
70	5356.0
71	4163.0
72	2970.0
73	2310.5
74	1651.0
75	1651.0
76	1244.5
77	838.0
78	673.0
79	508.0
80	379.5
81	251.0
82	251.0
83	175.5
84	100.0
85	79.5
86	59.0
87	42.5
88	26.0
89	26.0
90	16.0
91	6.0
92	3.0
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	902885.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.03199058147802
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.9895016400427	42.42002497591865
2	11.65962217125281	12.366659463389427
3	3.4257764712545145	5.450272366734553
4	1.461897012844115	3.1010923446495986
5	0.7603490385497668	2.0161411525503548
6	0.5094254424596817	1.6209507159892267
7	0.33374125217678263	1.2389274059462874
8	0.24628611036986034	1.0448834148386632
9	0.200759366476516	0.9582001948913482
>10	1.2397551568136131	12.83033598100529
>50	0.11100810313578527	4.05289999762006
>100	0.056652032390817224	5.036698887894131
>500	0.002717625161172243	1.098091705905669
>1k	0.0016723847145675341	1.929559428701481
>5k	4.1809617864188353E-4	1.7366589170128524
>10k+	4.1809617864188353E-4	3.0986030469523924
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT	15006	1.662005681786717	No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG	12944	1.433626652342214	No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT	8865	0.9818526168891941	Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC	6800	0.7531413192156254	No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC	3310	0.3666026127358412	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2859	0.3166516222996284	No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC	2826	0.31299667177990553	Illumina PCR Primer Index 11 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2612	0.28929487143988436	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1861	0.20611705809709985	No Hit
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG	1680	0.1860702082768016	No Hit
TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTA	1197	0.13257502339722113	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1060	0.11740144093655339	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	991	0.10975927166804189	No Hit
TATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTAT	965	0.10687961368280569	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	960	0.10632583330102947	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.1075607635523903E-4	0.0
3	0.0	0.0	0.0	1.1075607635523903E-4	0.0
4	0.0	0.0	0.0	1.1075607635523903E-4	0.0
5	0.0	0.0	0.0	1.1075607635523903E-4	0.0
6	0.0	0.0	0.0	1.1075607635523903E-4	0.0
7	0.0	0.0	0.0	1.1075607635523903E-4	0.0
8	0.0	0.0	0.0	1.1075607635523903E-4	0.0
9	0.0	0.0	0.0	1.1075607635523903E-4	0.0
10	0.0	0.0	0.0	2.2151215271047807E-4	0.0
11	0.0	0.0	0.0	2.2151215271047807E-4	0.0
12	0.0	0.0	0.0	2.2151215271047807E-4	0.0
13	0.0	0.0	0.0	2.2151215271047807E-4	0.0
14	0.0	0.0	0.0	2.2151215271047807E-4	0.0
15	0.0	0.0	0.0	2.2151215271047807E-4	0.0
16	0.0	0.0	0.0	2.2151215271047807E-4	0.0
17	0.0	0.0	0.0	2.2151215271047807E-4	0.0
18	0.0	0.0	0.0	3.322682290657171E-4	0.0
19	0.0	0.0	0.0	3.322682290657171E-4	0.0
20	0.0	0.0	0.0	4.4302430542095614E-4	0.0
21	0.0	0.0	0.0	6.645364581314342E-4	0.0
22	0.0	0.0	0.0	9.968046871971514E-4	0.0
23	0.0	0.0	0.0	0.0017720972216838245	0.0
24	0.0	0.0	0.0	0.002104365450749542	0.0
25	0.0	0.0	0.0	0.00232587760346002	0.0
26	0.0	0.0	0.0	0.002879657985236215	0.0
27	0.0	0.0	0.0	0.003654950519722888	0.0
28	0.0	0.0	0.0	0.008749730032063884	0.0
29	0.0	0.0	0.0	0.01927155728581159	0.0
30	0.0	0.0	0.0	0.034888164051900296	0.0
31	0.0	0.0	0.0	0.06789347480576154	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1710	0.0	33.213448	1
TTATACA	1875	0.0	29.698668	2
CGTCTTC	375	0.0	29.106665	37
TATACAC	1975	0.0	28.944302	3
CCGTCTT	1190	0.0	26.117647	37
GCCGTCT	1215	0.0	25.580248	36
GTATCAA	1555	0.0	24.983923	1
GGTATCA	555	0.0	24.0	1
CGGATCG	50	2.7014807E-4	22.199999	37
CCGCGTG	50	2.7014807E-4	22.199999	37
TCTATAC	100	5.347829E-10	22.199999	3
GCCGCGT	60	3.7251477E-5	21.583332	36
AATTCCG	45	0.0038251826	20.555555	14
ACTCACG	45	0.0038251826	20.555555	29
GCGAGGT	45	0.0038251826	20.555555	21
CGGTGTA	55	5.1419187E-4	20.181818	34
AGGGACG	65	6.9000336E-5	19.923077	32
TAAACCG	75	9.262161E-6	19.733334	5
TCGTCTT	95	1.6751255E-7	19.473684	14
GATTACG	125	4.110916E-10	19.240002	1
>>END_MODULE