FastQCFastQC Report
Fri 10 Feb 2017
ERR1631036.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631036.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200848
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT94814.720485142993707RNA PCR Primer, Index 46 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG92694.614932685413845No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT90734.517346451047558No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC51632.5706006532303034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40532.0179439177885765No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36901.8372102286306062No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC30501.5185613000876284RNA PCR Primer, Index 46 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC29571.472257627658727No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA18010.8966980004779733No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14320.7129769776149127No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA12670.6308253007249264No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG12260.6104118537401418No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9740.4849438381263443No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9370.4665219469449534No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA9260.461045168485621No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7970.396817493826177No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7400.3684378236278181No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7370.3669441567752728No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT7350.36594837887357606No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT7220.35947582251254684No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG6860.3415518202820043No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC6800.3385644865769139No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6750.3360750418226719No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5290.2633832549988051No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5230.26039592129371464No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5150.2564128096869274No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5040.250936031227595No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5020.2499402533258982No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4840.24097825221062694No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4610.22952680634111366No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG4310.21459013781566158No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4190.20861547040548079No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4120.20513024774954197No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4050.20164502509360313No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3990.19865769138851272No Hit
ATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT3910.1946745797817255No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3910.1946745797817255No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG3780.18820202342069625No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3780.18820202342069625No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG3720.1852146897156058No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3690.1837210228630606No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3620.1802358002071218No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3570.17774635545287978No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3310.16480124273082133No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3290.1638054648291245No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3290.1638054648291245No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3260.1623117979765793No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3220.1603202421731857No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC3220.1603202421731857No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3200.15932446427148889No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3180.15832868636979208No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3170.15783079741894368No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG3110.15484346371385327No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2970.14787301840197561No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA2960.14737512945112724No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA2940.1463793515494304No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2870.1428941288934916No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2750.13691946148331077No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC2630.13094479407312992No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG2570.1279574603680395No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG2500.1244722377121007No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2460.12248068190870709No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2450.12198279295785867No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT2440.12148490400701027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC2400.11949334820361666No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT2320.11551023659682944No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG2310.11501234764598105No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA2300.11451445869513263No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA2280.11351868079343584No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT2270.11302079184258743No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG2260.11252290289173904No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2260.11252290289173904No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA2240.1115271249900422No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT2220.11053134708834542No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT2190.1090376802358002No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC2080.10356090177646778No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG2050.10206723492392257No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA2030.10107145702222577No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC2020.10057356807137735No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA2010.10007567912052896No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA2010.10007567912052896No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCACCA251.2300003E-437.031
TAGGACC251.2300003E-437.04
GAAGTTA251.2300003E-437.023
CATTTTG308.277842E-637.036
GCGTGCC251.2300003E-437.09
TGCCTTC251.2300003E-437.012
ATTACGG501.7826096E-1037.02
TAGTATT200.001838321237.02
GACCGTT251.2300003E-437.07
CACCATT251.2300003E-437.033
CGTGCCT251.2300003E-437.010
GATTACG501.7826096E-1037.01
GCACCAT251.2300003E-437.032
CGTCTTC3600.037.037
TTACGGG551.2732926E-1137.03
GTAGTAT200.001838321237.01
GTGCCTT251.2300003E-437.011
CCGAAGT251.2300003E-437.021
CTTATAC11700.034.3119661
CAGACAC451.0724216E-732.8888918
Produced by FastQC (version 0.11.5)