FastQCFastQC Report
Fri 10 Feb 2017
ERR1631035.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631035.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157578
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT121427.705390346368148No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG109366.940055083831499No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT94656.006549137569965RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC57053.6204292477376283No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC39682.5181180113975303RNA PCR Primer, Index 27 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC29981.9025498483290815RNA PCR Primer, Index 27 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22911.4538831562781607No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20611.3079236949320336No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG13160.8351419614413179No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12090.767239081597685No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA10720.6802980111436876No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTAT8990.5705111119572529No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8470.5375115815659547No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7690.4880122859790072No Hit
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT7400.46960870172232166No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6140.38964830115879123No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5820.3693408978410692No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5120.32491845308355227No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5100.32364924037619464No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4830.3065148688268667No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4530.2874766782165023No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4520.2868420718628235No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG4340.2754191574966049No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3510.22274683014126337No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG3360.21322773483608118No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3120.19799718234778965No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3060.1941895442257168No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3000.19038190610364392No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG2990.1897472997499651No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG2990.1897472997499651No Hit
ATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT2970.18847808704260746No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT2960.18784348068892864No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2880.18276662985949815No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG2810.17832438538374645No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG2700.17134371549327954No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2660.16880529007856426No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2610.1656322583101702No Hit
TCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT2600.16499765195649138RNA PCR Primer, Index 27 (100% over 23bp)
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG2560.16245922654177614No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2470.15674776935866683No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2440.15484395029763037No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG2390.15167091852923634No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2280.14469024863876936No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC2250.14278642957773294No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT2150.1364403660409448No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT2090.13263272791887193No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2060.13072890885783547No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA2060.13072890885783547No Hit
CTTTACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA1940.12311363261368972No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC1940.12311363261368972No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1930.1224790262600109No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA1860.11803678178425922No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG1840.1167675690769016No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG1700.10788308012539823No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG1670.10597926106436178No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.10471004835700415No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT1640.10407544200332534No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC1630.10344083564964653No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG1610.10217162294228889No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA1600.10153701658861009No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1600.10153701658861009No Hit
TCGCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT1590.10090241023493127RNA PCR Primer, Index 27 (95% over 23bp)
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC1590.10090241023493127No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA200.001837119637.01
GATACAC200.001837119637.03
TGATACA200.001837119637.02
TTACGGG308.2665065E-637.03
CTTATAC13100.035.4465681
CGTCTTC4300.033.1279137
TTATACA14100.032.8014222
ATTACGG352.3753444E-531.7142872
GATTACG352.3753444E-531.7142871
TATACAC14750.031.355933
CGGAGAT250.005482137729.5999981
GATATGT250.005482137729.5999981
TATTGTA250.005482137729.59999831
ATTGTAA250.005482137729.59999832
CAGACAC453.979465E-628.77777918
ACGTCAT358.8353246E-426.42857235
CCGTCTT14300.026.00349637
GACACAT509.026247E-625.89999820
CAGTGGG509.026247E-625.89999824
AGCAGTG659.2846676E-825.61538522
Produced by FastQC (version 0.11.5)