##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631035.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	157578
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17094391349046	31.0	31.0	34.0	28.0	34.0
2	31.263761438779525	31.0	31.0	34.0	28.0	34.0
3	30.712961200167538	31.0	31.0	34.0	26.0	34.0
4	34.818673926563356	37.0	35.0	37.0	32.0	37.0
5	35.04175075200853	35.0	35.0	37.0	32.0	37.0
6	35.255740014469026	37.0	35.0	37.0	32.0	37.0
7	35.220037061011055	37.0	35.0	37.0	32.0	37.0
8	35.25766287172067	37.0	35.0	37.0	32.0	37.0
9	37.08491033012223	39.0	37.0	39.0	33.0	39.0
10	36.90039853279011	39.0	37.0	39.0	33.0	39.0
11	36.96965312416708	39.0	37.0	39.0	33.0	39.0
12	36.808101384711065	39.0	37.0	39.0	32.0	39.0
13	36.9394331696049	39.0	37.0	39.0	33.0	39.0
14	37.69360570638033	39.0	37.0	41.0	32.0	41.0
15	37.688357511835406	39.0	37.0	41.0	32.0	41.0
16	37.718774194367235	39.0	37.0	41.0	32.0	41.0
17	37.62377996928505	39.0	37.0	41.0	32.0	41.0
18	37.86159235426265	39.0	37.0	41.0	33.0	41.0
19	37.73911332800264	39.0	37.0	41.0	32.0	41.0
20	37.61215398088566	39.0	37.0	41.0	32.0	41.0
21	37.41031743009811	39.0	36.0	41.0	32.0	41.0
22	37.359491807231976	39.0	36.0	40.0	31.0	41.0
23	37.47090329868383	39.0	36.0	41.0	32.0	41.0
24	37.37005800302072	39.0	36.0	41.0	31.0	41.0
25	37.26754369264745	39.0	36.0	40.0	31.0	41.0
26	36.9906332102197	39.0	36.0	40.0	31.0	41.0
27	36.814504562819685	39.0	35.0	40.0	30.0	41.0
28	36.7126438969907	39.0	35.0	40.0	30.0	41.0
29	36.860697559303965	39.0	35.0	40.0	31.0	41.0
30	36.64427775450888	39.0	35.0	40.0	30.0	41.0
31	36.31582454403534	38.0	35.0	40.0	30.0	41.0
32	36.29414639099367	38.0	35.0	40.0	30.0	41.0
33	36.23319245072282	38.0	35.0	40.0	30.0	41.0
34	36.1337750193555	38.0	35.0	40.0	30.0	41.0
35	36.20707839926893	38.0	35.0	40.0	30.0	41.0
36	36.084751678533806	38.0	35.0	40.0	30.0	41.0
37	35.87029915343513	38.0	35.0	40.0	29.0	41.0
38	35.77566665397454	38.0	34.0	40.0	28.0	41.0
39	35.48764421429387	38.0	34.0	40.0	27.0	41.0
40	35.082771706710325	38.0	34.0	40.0	26.0	41.0
41	35.05520440670652	38.0	34.0	40.0	26.0	41.0
42	34.865514221528386	38.0	34.0	40.0	25.0	41.0
43	33.74514208836259	37.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	8.0
10	5.0
11	15.0
12	13.0
13	4.0
14	8.0
15	4.0
16	4.0
17	8.0
18	20.0
19	12.0
20	33.0
21	80.0
22	142.0
23	298.0
24	461.0
25	796.0
26	1172.0
27	1747.0
28	2478.0
29	3374.0
30	4240.0
31	5259.0
32	6453.0
33	8069.0
34	10075.0
35	12953.0
36	17072.0
37	24245.0
38	33416.0
39	25113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.14760943786569	24.050946198073337	18.285547474901318	27.515896889159652
2	10.884768178299002	20.024368883981268	44.6864410006473	24.404421937072435
3	12.535379304217594	31.58055058447245	37.82190407290358	18.062166038406378
4	8.402188122707486	21.67307619083882	26.011879830940863	43.91285585551283
5	13.14206297833454	36.97216616532765	30.454758913046238	19.431011943291576
6	25.1602381043039	37.08258767086776	15.078246963408597	22.67892726141974
7	20.309941743136733	34.99854040538654	14.604196017210525	30.087321834266206
8	27.157978905684804	28.593458477706278	25.07520085291094	19.173361763697976
9	18.465141072992424	11.010420236327406	13.478404345784309	57.04603434489586
10	14.875807536585056	26.92380916117732	26.735965680488395	31.46441762174923
11	28.327558415514858	18.86240465039536	23.05778725456599	29.75224967952379
12	17.485308862912337	27.30901521786036	19.39039713665613	35.81527878257117
13	36.15479318178934	14.672098897054155	24.318115472972117	24.85499244818439
14	23.018441660637905	26.064552158296213	20.20459708842605	30.712409092639838
15	29.25535290459328	24.489459188465396	16.729492695680868	29.525695211260455
16	31.524705225348715	21.168564139664166	15.944484636180178	31.36224599880694
17	17.454847757935752	36.43528919011537	16.853240934648237	29.25662211730064
18	30.231377476551298	14.907537854268996	19.595374988894392	35.26570968028532
19	32.44424983182931	24.847377171940245	19.491299546891064	23.217073449339377
20	25.32079351178464	21.046719719757835	19.909505133965403	33.72298163449212
21	30.68892865755372	22.93403901559862	20.05292616989681	26.324106156950844
22	18.82559748188199	35.70231885161634	22.199164858038557	23.27291880846311
23	25.664750155478554	17.76643947759205	26.840675728845397	29.728134638083997
24	28.865069997080813	23.43855106677328	20.33849902905228	27.35787990709363
25	20.502227468301413	26.28222213760804	24.52372793156405	28.691822462526495
26	31.835662338651332	23.1923238015459	26.600159920801126	18.371853939001635
27	24.63161101168945	30.043533995862365	24.007792966023175	21.31706202642501
28	15.455837743847493	24.483747731282286	32.77234131668126	27.288073208188962
29	25.387427178920912	18.44673748873574	21.658480244704208	34.507355087639134
30	23.310360583330162	17.462463034179898	37.362449072840114	21.864727309649822
31	20.88933734404549	23.215804236632017	23.83454543146886	32.060312987853635
32	20.16080925002221	19.40055083831499	26.67313965147419	33.7655002601886
33	15.09411212225057	18.262067039815204	47.259135158461206	19.384685679473023
34	15.665892446915178	29.441292566221172	24.393633629059895	30.499181357803756
35	17.55321174275597	17.837515389204075	40.183908921296116	24.425363946743836
36	22.75888766198327	18.756425389330996	29.540291157395064	28.94439579129066
37	20.647552323293862	25.02062470649456	29.622789983373316	24.709032986838263
38	20.09163715747122	16.567668075492772	31.636395943596185	31.70429882343982
39	29.94707383010319	18.40231504397822	24.74330172993692	26.90730939598167
40	16.875452157026995	17.02648846920255	44.688344819708334	21.409714554062116
41	23.646067344426253	21.48142507202782	22.90421251697572	31.968295066570207
42	24.28321212351978	16.488342281282918	38.198225640635115	21.030219954562185
43	24.467247966086635	16.92875909073602	34.27953140666845	24.324461536508903
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	2.5
2	4.0
3	32.0
4	60.0
5	60.0
6	92.0
7	124.0
8	151.5
9	179.0
10	275.0
11	371.0
12	371.0
13	565.5
14	760.0
15	2024.5
16	3289.0
17	3309.0
18	3329.0
19	3329.0
20	2745.5
21	2162.0
22	1661.0
23	1160.0
24	1083.5
25	1007.0
26	1007.0
27	1019.0
28	1031.0
29	980.5
30	930.0
31	984.0
32	1038.0
33	1038.0
34	1212.5
35	1387.0
36	1358.0
37	1329.0
38	1477.5
39	1626.0
40	1626.0
41	2103.5
42	2581.0
43	3529.0
44	4477.0
45	6145.5
46	7814.0
47	7814.0
48	9882.0
49	11950.0
50	12849.0
51	13748.0
52	25410.0
53	37072.0
54	37072.0
55	24911.0
56	12750.0
57	19173.5
58	25597.0
59	19923.0
60	14249.0
61	14249.0
62	8904.5
63	3560.0
64	2710.5
65	1861.0
66	1498.5
67	1136.0
68	1136.0
69	899.0
70	662.0
71	454.5
72	247.0
73	145.0
74	43.0
75	43.0
76	32.0
77	21.0
78	17.0
79	13.0
80	8.5
81	4.0
82	4.0
83	2.5
84	1.0
85	3.0
86	5.0
87	2.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	157578.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.975504194747998
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.81189439908505	14.544543019964715
2	10.499729961559234	4.194747997816954
3	4.552530419036121	2.728172714465217
4	2.646376719509483	2.114508370457805
5	1.6519998729230867	1.6499765195649139
6	1.1246306827207166	1.347903895213799
7	0.89589223877752	1.252712942161977
8	0.6067922610159799	0.9696785084212264
9	0.4860691933792928	0.8738529490157255
>10	3.888553547034342	15.7071418599043
>50	0.4860691933792928	6.553579814441102
>100	0.28909997776154017	10.852403254261382
>500	0.02541538266035518	3.473200573684144
>1k	0.022238459827810784	9.465153765119497
>5k	0.006353845665088795	9.626978385307595
>10k+	0.006353845665088795	14.645445430199647
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT	12142	7.705390346368148	No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG	10936	6.940055083831499	No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT	9465	6.006549137569965	RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC	5705	3.6204292477376283	No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC	3968	2.5181180113975303	RNA PCR Primer, Index 27 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC	2998	1.9025498483290815	RNA PCR Primer, Index 27 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2291	1.4538831562781607	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2061	1.3079236949320336	No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG	1316	0.8351419614413179	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1209	0.767239081597685	No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA	1072	0.6802980111436876	No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTAT	899	0.5705111119572529	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	847	0.5375115815659547	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	769	0.4880122859790072	No Hit
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT	740	0.46960870172232166	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	614	0.38964830115879123	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	582	0.3693408978410692	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	512	0.32491845308355227	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	510	0.32364924037619464	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	483	0.3065148688268667	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	453	0.2874766782165023	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	452	0.2868420718628235	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	434	0.2754191574966049	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	351	0.22274683014126337	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	336	0.21322773483608118	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	312	0.19799718234778965	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	306	0.1941895442257168	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	300	0.19038190610364392	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	299	0.1897472997499651	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	299	0.1897472997499651	No Hit
ATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT	297	0.18847808704260746	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	296	0.18784348068892864	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	288	0.18276662985949815	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	281	0.17832438538374645	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	270	0.17134371549327954	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	266	0.16880529007856426	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	261	0.1656322583101702	No Hit
TCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT	260	0.16499765195649138	RNA PCR Primer, Index 27 (100% over 23bp)
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	256	0.16245922654177614	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	247	0.15674776935866683	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	244	0.15484395029763037	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	239	0.15167091852923634	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	228	0.14469024863876936	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	225	0.14278642957773294	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	215	0.1364403660409448	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	209	0.13263272791887193	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	206	0.13072890885783547	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	206	0.13072890885783547	No Hit
CTTTACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA	194	0.12311363261368972	No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	194	0.12311363261368972	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	193	0.1224790262600109	No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA	186	0.11803678178425922	No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG	184	0.1167675690769016	No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG	170	0.10788308012539823	No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG	167	0.10597926106436178	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	165	0.10471004835700415	No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT	164	0.10407544200332534	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	163	0.10344083564964653	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	161	0.10217162294228889	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	160	0.10153701658861009	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	160	0.10153701658861009	No Hit
TCGCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT	159	0.10090241023493127	RNA PCR Primer, Index 27 (95% over 23bp)
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC	159	0.10090241023493127	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	6.34606353678813E-4	0.0
11	0.0	0.0	0.0	0.001269212707357626	0.0
12	0.0	0.0	0.0	0.001269212707357626	0.0
13	0.0	0.0	0.0	0.001269212707357626	0.0
14	0.0	0.0	0.0	0.001269212707357626	0.0
15	0.0	0.0	0.0	0.001903819061036439	0.0
16	0.0	0.0	0.0	0.002538425414715252	0.0
17	0.0	0.0	0.0	0.002538425414715252	0.0
18	0.0	0.0	0.0	0.003173031768394065	0.0
19	0.0	0.0	0.0	0.003173031768394065	0.0
20	0.0	0.0	0.0	0.003173031768394065	0.0
21	0.0	0.0	0.0	0.004442244475751691	0.0
22	0.0	0.0	0.0	0.005711457183109317	0.0
23	0.0	0.0	0.0	0.00634606353678813	0.0
24	0.0	0.0	0.0	0.007615276244145756	0.0
25	0.0	0.0	0.0	0.007615276244145756	0.0
26	0.0	0.0	0.0	0.00824988259782457	0.0
27	0.0	0.0	0.0	0.010153701658861009	0.0
28	0.0	0.0	0.0	0.02474964779347371	0.0
29	0.0	0.0	0.0	0.05267232735534148	0.0
30	0.0	0.0	0.0	0.09392174034446434	0.0
31	0.0	0.0	0.0	0.14786328040716343	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACA	20	0.0018371196	37.0	1
GATACAC	20	0.0018371196	37.0	3
TGATACA	20	0.0018371196	37.0	2
TTACGGG	30	8.2665065E-6	37.0	3
CTTATAC	1310	0.0	35.446568	1
CGTCTTC	430	0.0	33.12791	37
TTATACA	1410	0.0	32.801422	2
ATTACGG	35	2.3753444E-5	31.714287	2
GATTACG	35	2.3753444E-5	31.714287	1
TATACAC	1475	0.0	31.35593	3
CGGAGAT	25	0.0054821377	29.599998	1
GATATGT	25	0.0054821377	29.599998	1
TATTGTA	25	0.0054821377	29.599998	31
ATTGTAA	25	0.0054821377	29.599998	32
CAGACAC	45	3.979465E-6	28.777779	18
ACGTCAT	35	8.8353246E-4	26.428572	35
CCGTCTT	1430	0.0	26.003496	37
GACACAT	50	9.026247E-6	25.899998	20
CAGTGGG	50	9.026247E-6	25.899998	24
AGCAGTG	65	9.2846676E-8	25.615385	22
>>END_MODULE