FastQCFastQC Report
Fri 10 Feb 2017
ERR1631034.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631034.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914422
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT129751.4189291158786643No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG113951.2461423719026883No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT92551.0121147566440878Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC63190.6910376172051854No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC35520.3884420978497892Illumina PCR Primer Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC31240.34163657479806914No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC27090.29625271482969573No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT24390.2667258661755732No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA23110.25272795273954474No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC23040.25196244184851196No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC22770.2490097569830997No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22190.2426669524573993No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT21560.23577735443810408No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20810.22757545203418114No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT19430.21248395161096298No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA18740.20493820139935393No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG18520.20253231002753652No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA16900.18481620083506303No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG16160.17672365712985907No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA15730.17202123308494327No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG15220.1664439394502757No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT15010.16414740677717726No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC14820.1620695915015168No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC14820.1620695915015168No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA14320.15660165656556818No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG13400.14654065628342275No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT12940.14151015614235005No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC12900.14107272134747414No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG12490.1365890146999963No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG12340.13494863421921172No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC11880.12991813407813899No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA11720.1281683948986354No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA11340.12401276434731447No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA10650.11646701413570539No Hit
TTGTAATGCTGGTACAAGGCAGCTGGCAACGTTCCCTTCAAGA10420.11395176406516903No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC10410.11384240536645007No Hit
ATGCAAAGCAATGTGGCCTCAGAATACACCTCTTAAATTTACA10320.11285817707797931No Hit
GCATTTACTTTGTGGCTGGATTATTTGTAATGCTGGTACAAGG9950.10881190522537734No Hit
TGCTGGTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGA9780.10695280734715482No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG9720.10629665515484099No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA9570.10465627467405639No Hit
CTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGTCT9470.10356268768686669No Hit
GGAAATAACTATATCACTATTCAAGATCATCTTCACAACATCA9450.10334397028942874No Hit
ACACAACACTAAAAGAAAATTTATTTATTGGCATGCAAAGCAA9230.10093807891761133No Hit
TATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTAT9220.10082872021889236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC14900.033.523491
TAACGGT303.5989084E-430.83333230
TTATACA16750.030.2626862
CTGGCGA250.005495762529.637
GATTACG454.007079E-628.7777771
TATACAC18050.028.2880883
TTACGGG604.3208274E-827.7499983
TGCACGC604.3208274E-827.74999837
CGTCTTC5450.027.49541337
GGTATCA4400.026.909091
GCGATAG358.868005E-426.4285736
CCGTCTT15900.025.24842637
GCCGTCT16200.024.78086536
TCGCCGA451.3229233E-424.6666661
GTATCAA12900.024.0930211
ATTACGG551.9021072E-523.5454542
CGGAGAG502.7015165E-422.226
TCGCCAT3050.021.2295113
TGCCGTC19750.020.60759535
CATCGAA450.00382521920.55555526
Produced by FastQC (version 0.11.5)