##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631034.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	914422
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47135567604454	31.0	31.0	34.0	28.0	34.0
2	31.48247308135631	31.0	31.0	34.0	28.0	34.0
3	30.915871446662482	31.0	31.0	34.0	27.0	34.0
4	35.05570732112744	37.0	35.0	37.0	32.0	37.0
5	35.284058126335545	37.0	35.0	37.0	33.0	37.0
6	35.48416595401248	37.0	35.0	37.0	33.0	37.0
7	35.49754817797472	37.0	35.0	37.0	33.0	37.0
8	35.52085798460667	37.0	35.0	37.0	33.0	37.0
9	37.29142124752029	39.0	37.0	39.0	34.0	39.0
10	37.138042391805975	39.0	37.0	39.0	33.0	39.0
11	37.18559920911789	39.0	37.0	39.0	33.0	39.0
12	37.1276423795578	39.0	37.0	39.0	33.0	39.0
13	37.187962450597205	39.0	37.0	39.0	33.0	39.0
14	38.331658687126954	40.0	38.0	41.0	34.0	41.0
15	38.354169081671266	40.0	38.0	41.0	34.0	41.0
16	38.33942206114901	40.0	38.0	41.0	34.0	41.0
17	38.339872618987734	40.0	38.0	41.0	34.0	41.0
18	38.361137417953636	40.0	38.0	41.0	34.0	41.0
19	38.41013448932768	40.0	38.0	41.0	34.0	41.0
20	38.39947857772451	40.0	38.0	41.0	34.0	41.0
21	38.34758678159537	40.0	38.0	41.0	34.0	41.0
22	38.292278619718246	40.0	38.0	41.0	34.0	41.0
23	38.29298179615101	40.0	38.0	41.0	34.0	41.0
24	38.2611999711293	40.0	38.0	41.0	34.0	41.0
25	38.24414876282504	40.0	38.0	41.0	34.0	41.0
26	38.13311796960266	40.0	38.0	41.0	33.0	41.0
27	38.073513104452864	40.0	37.0	41.0	33.0	41.0
28	38.051091290454515	40.0	37.0	41.0	33.0	41.0
29	37.99260625837961	40.0	37.0	41.0	33.0	41.0
30	37.93995988722931	40.0	37.0	41.0	33.0	41.0
31	37.88793795424869	40.0	37.0	41.0	33.0	41.0
32	37.803217770351104	40.0	37.0	41.0	33.0	41.0
33	37.80515888725337	40.0	37.0	41.0	33.0	41.0
34	37.75156000183723	40.0	37.0	41.0	33.0	41.0
35	37.76239198094534	40.0	37.0	41.0	33.0	41.0
36	37.73763098438139	40.0	37.0	41.0	33.0	41.0
37	37.67752416280448	40.0	37.0	41.0	32.0	41.0
38	37.625993250381114	40.0	37.0	41.0	32.0	41.0
39	37.55725474671432	40.0	37.0	41.0	32.0	41.0
40	37.4896109236217	40.0	37.0	41.0	32.0	41.0
41	37.457311831955046	40.0	37.0	41.0	32.0	41.0
42	37.42061761418689	39.0	37.0	41.0	32.0	41.0
43	36.783077178808036	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	12.0
11	14.0
12	10.0
13	2.0
14	10.0
15	7.0
16	4.0
17	7.0
18	17.0
19	33.0
20	64.0
21	152.0
22	288.0
23	556.0
24	1017.0
25	1792.0
26	2890.0
27	4651.0
28	6914.0
29	10377.0
30	14185.0
31	19258.0
32	25704.0
33	33444.0
34	43939.0
35	59728.0
36	83247.0
37	122938.0
38	217174.0
39	265980.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.644107425236925	23.16468763874885	15.57267869758164	23.618526238432583
2	13.354884287560886	22.92726990382996	41.78738044360262	21.93046536500653
3	14.361531109269023	28.546885354901786	35.99574375944586	21.095839776383333
4	11.907521910015289	19.55125751567657	37.78824219014853	30.75297838415961
5	11.739218872686791	39.70748735266649	36.01378794473449	12.539505829912228
6	26.748590913167007	40.34920419674942	18.586823151673954	14.315381738409618
7	22.857389695348537	34.682345787831004	23.52436839883555	18.935896117984914
8	23.341630013276145	36.97428539558322	22.380804486331257	17.303280104809378
9	23.139207061947328	16.383136013787944	19.879989764025797	40.59766716023893
10	13.596785729127253	29.894403240516958	34.4546609770981	22.054150053257686
11	29.931366480683973	24.0958769583409	26.28753463936782	19.685221921607308
12	19.512544536330054	27.37259164805746	29.83884902156772	23.276014794044762
13	27.210740773953386	21.77375434974224	28.2789565430403	22.73654833326407
14	20.663654199045954	23.377937101250843	27.273184590921918	28.68522410878128
15	23.262673032801047	29.601868721443708	24.297315681381242	22.838142564373996
16	23.752271926965886	29.039655651329472	23.879456093576053	23.328616328128586
17	20.76404548446997	31.121626557541266	25.35787634155784	22.756451616430926
18	21.766099240831913	27.554345805328396	26.798349121084136	23.881205832755555
19	23.52830531198943	28.15264724601989	27.077541878913674	21.241505563077006
20	23.757958579299274	26.326247618714337	27.491573912263707	22.42421988972269
21	23.256002152179192	27.85595709639532	25.813574039119793	23.074466712305696
22	20.399662300338356	32.16873609777542	26.16417802721282	21.2674235746734
23	22.80238226989289	27.39632248567948	26.42128032790112	23.380014916526505
24	23.408229460796	28.66980453226191	25.774423624978404	22.14754238196369
25	22.86876300001531	27.74473929979812	26.789819142584058	22.596678557602505
26	23.097978832530274	26.756902174269648	27.168856392344015	22.97626260085606
27	21.984379203474983	29.409834846493194	27.0171758772208	21.588610072811022
28	21.536555332220793	27.17038741412608	28.30782723950211	22.985230014151014
29	21.097042722069244	28.96791634496983	26.261069834277826	23.673971098683104
30	22.901023816137407	28.010480937685227	27.424318312551538	21.664176933625832
31	23.219148270710896	26.78960042518662	27.23458097027412	22.756670333828364
32	21.143082734229928	29.184009133638515	27.373575876345935	22.29933225578562
33	21.821981535877306	26.677507758999674	30.044880809954265	21.45562989516875
34	20.171321337413143	28.96616660579032	27.08530634652272	23.77720571027381
35	21.072327656158755	27.092742738035614	29.538550034885425	22.29637957092021
36	21.99870519300717	27.44925209585946	27.323708309730083	23.22833440140329
37	22.499021239646467	27.231409568011266	27.83124203048483	22.438327161857437
38	22.2571197980801	26.731202880070686	28.3665528607142	22.645124461135012
39	23.11241418076118	27.005365137759153	27.423115366865623	22.45910531461404
40	21.10765051584498	25.928619390172152	30.859384398013173	22.104345695969695
41	21.50571617918204	27.794169431619096	27.386152126698615	23.313962262500247
42	22.74868714882188	25.928400672774714	29.439908488640913	21.883003689762496
43	21.867693471941838	26.070566981109376	29.268105972953407	22.793633573995375
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10.0
1	16.0
2	22.0
3	69.0
4	116.0
5	116.0
6	170.0
7	224.0
8	231.5
9	239.0
10	404.0
11	569.0
12	569.0
13	809.0
14	1049.0
15	3016.0
16	4983.0
17	5518.0
18	6053.0
19	6053.0
20	6861.0
21	7669.0
22	10095.5
23	12522.0
24	15237.0
25	17952.0
26	17952.0
27	22493.0
28	27034.0
29	35035.5
30	43037.0
31	44018.0
32	44999.0
33	44999.0
34	48383.5
35	51768.0
36	53063.5
37	54359.0
38	60517.5
39	66676.0
40	66676.0
41	71672.0
42	76668.0
43	68206.5
44	59745.0
45	61080.5
46	62416.0
47	62416.0
48	62230.5
49	62045.0
50	63124.5
51	64204.0
52	66455.5
53	68707.0
54	68707.0
55	67123.5
56	65540.0
57	48223.5
58	30907.0
59	33925.5
60	36944.0
61	36944.0
62	30216.5
63	23489.0
64	16642.5
65	9796.0
66	7956.0
67	6116.0
68	6116.0
69	4804.5
70	3493.0
71	3020.0
72	2547.0
73	1935.0
74	1323.0
75	1323.0
76	960.0
77	597.0
78	474.5
79	352.0
80	247.0
81	142.0
82	142.0
83	103.5
84	65.0
85	47.5
86	30.0
87	19.5
88	9.0
89	9.0
90	7.5
91	6.0
92	3.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	914422.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.275536756212745
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.49337469046269	32.811317089115846
2	12.050007285961692	9.947410372886866
3	3.4573887106786314	4.281167244243925
4	1.4418390138951702	2.3805071685828656
5	0.76545927268651	1.579737117257796
6	0.4640803899560373	1.1493100316080778
7	0.31313399868434655	0.9047341710620934
8	0.2381133902187671	0.786260639209692
9	0.19623925424042282	0.7289892496271115
>10	1.2287640991186266	10.106117625849016
>50	0.1563554979972989	4.54053700509836
>100	0.1648789647828649	14.384603986784505
>500	0.020509984310569905	5.7168128136820435
>1k	0.008789993275958531	6.290934828241211
>5k	5.32726865209608E-4	1.7122512935167533
>10k+	5.32726865209608E-4	2.679309363233805
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT	12975	1.4189291158786643	No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG	11395	1.2461423719026883	No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT	9255	1.0121147566440878	Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC	6319	0.6910376172051854	No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC	3552	0.3884420978497892	Illumina PCR Primer Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC	3124	0.34163657479806914	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	2709	0.29625271482969573	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	2439	0.2667258661755732	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	2311	0.25272795273954474	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	2304	0.25196244184851196	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	2277	0.2490097569830997	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2219	0.2426669524573993	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	2156	0.23577735443810408	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2081	0.22757545203418114	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	1943	0.21248395161096298	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	1874	0.20493820139935393	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1852	0.20253231002753652	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	1690	0.18481620083506303	No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG	1616	0.17672365712985907	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1573	0.17202123308494327	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	1522	0.1664439394502757	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	1501	0.16414740677717726	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	1482	0.1620695915015168	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1482	0.1620695915015168	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	1432	0.15660165656556818	No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG	1340	0.14654065628342275	No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT	1294	0.14151015614235005	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	1290	0.14107272134747414	No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG	1249	0.1365890146999963	No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG	1234	0.13494863421921172	No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC	1188	0.12991813407813899	No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA	1172	0.1281683948986354	No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA	1134	0.12401276434731447	No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA	1065	0.11646701413570539	No Hit
TTGTAATGCTGGTACAAGGCAGCTGGCAACGTTCCCTTCAAGA	1042	0.11395176406516903	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	1041	0.11384240536645007	No Hit
ATGCAAAGCAATGTGGCCTCAGAATACACCTCTTAAATTTACA	1032	0.11285817707797931	No Hit
GCATTTACTTTGTGGCTGGATTATTTGTAATGCTGGTACAAGG	995	0.10881190522537734	No Hit
TGCTGGTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGA	978	0.10695280734715482	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	972	0.10629665515484099	No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA	957	0.10465627467405639	No Hit
CTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGTCT	947	0.10356268768686669	No Hit
GGAAATAACTATATCACTATTCAAGATCATCTTCACAACATCA	945	0.10334397028942874	No Hit
ACACAACACTAAAAGAAAATTTATTTATTGGCATGCAAAGCAA	923	0.10093807891761133	No Hit
TATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTAT	922	0.10082872021889236	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.093586987189722E-4	0.0
8	0.0	0.0	0.0	2.187173974379444E-4	0.0
9	0.0	0.0	0.0	5.46793493594861E-4	0.0
10	0.0	0.0	0.0	5.46793493594861E-4	0.0
11	1.093586987189722E-4	0.0	0.0	5.46793493594861E-4	0.0
12	1.093586987189722E-4	0.0	0.0	5.46793493594861E-4	0.0
13	1.093586987189722E-4	0.0	0.0	5.46793493594861E-4	0.0
14	1.093586987189722E-4	0.0	0.0	6.561521923138332E-4	0.0
15	1.093586987189722E-4	0.0	0.0	6.561521923138332E-4	0.0
16	1.093586987189722E-4	0.0	0.0	6.561521923138332E-4	0.0
17	1.093586987189722E-4	0.0	0.0	6.561521923138332E-4	0.0
18	1.093586987189722E-4	0.0	0.0	7.655108910328054E-4	0.0
19	1.093586987189722E-4	0.0	0.0	7.655108910328054E-4	0.0
20	1.093586987189722E-4	0.0	0.0	7.655108910328054E-4	0.0
21	1.093586987189722E-4	0.0	0.0	0.0013123043846276663	0.0
22	1.093586987189722E-4	0.0	0.0	0.0014216630833466387	0.0
23	1.093586987189722E-4	0.0	0.0	0.001640380480784583	0.0
24	1.093586987189722E-4	0.0	0.0	0.0017497391795035552	0.0
25	1.093586987189722E-4	0.0	0.0	0.0018590978782225276	0.0
26	1.093586987189722E-4	0.0	0.0	0.0019684565769414997	0.0
27	1.093586987189722E-4	0.0	0.0	0.0028433261666932774	0.0
28	1.093586987189722E-4	0.0	0.0	0.0071083154167331936	0.0
29	1.093586987189722E-4	0.0	0.0	0.017497391795035552	0.0
30	1.093586987189722E-4	0.0	0.0	0.030948511737469132	0.0
31	1.093586987189722E-4	0.0	0.0	0.06780239320576277	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1490	0.0	33.52349	1
TAACGGT	30	3.5989084E-4	30.833332	30
TTATACA	1675	0.0	30.262686	2
CTGGCGA	25	0.0054957625	29.6	37
GATTACG	45	4.007079E-6	28.777777	1
TATACAC	1805	0.0	28.288088	3
TTACGGG	60	4.3208274E-8	27.749998	3
TGCACGC	60	4.3208274E-8	27.749998	37
CGTCTTC	545	0.0	27.495413	37
GGTATCA	440	0.0	26.90909	1
GCGATAG	35	8.868005E-4	26.42857	36
CCGTCTT	1590	0.0	25.248426	37
GCCGTCT	1620	0.0	24.780865	36
TCGCCGA	45	1.3229233E-4	24.666666	1
GTATCAA	1290	0.0	24.093021	1
ATTACGG	55	1.9021072E-5	23.545454	2
CGGAGAG	50	2.7015165E-4	22.2	26
TCGCCAT	305	0.0	21.22951	13
TGCCGTC	1975	0.0	20.607595	35
CATCGAA	45	0.003825219	20.555555	26
>>END_MODULE