FastQCFastQC Report
Fri 10 Feb 2017
ERR1631033.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631033.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186648
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT133607.157858643007158No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG113306.07024988213107No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT93415.0046076036175045RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC57553.083344048690583No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC35371.8950109296643949RNA PCR Primer, Index 26 (100% over 23bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC31491.6871329990141872No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27831.4910419613389911No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26181.4026402640264026No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15420.8261540439758263No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG12930.6927478462131927No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA11540.6182761133256183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10100.5411255411255411No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTAT9690.5191590587630192No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9380.5025502550255025No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8800.4714757190004715No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT8560.4586172903004586No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT7570.4055762719129056RNA PCR Primer, Index 26 (95% over 21bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7090.37985941451287986No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6930.3712871287128713No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG6920.37075136085037075No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT6750.36164330718786164No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC6020.32253225322532253No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5700.3053876816253054No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4670.2502035917877502No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4430.23734516308773734No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4270.2287728772877288No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG4230.22662980583772666No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3980.21323560927521326No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3960.21216407355021216No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3840.20573485920020573No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG3730.19984141271269987No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3540.18966182332518966No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3470.1859114482876859No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG3430.18376837683768377No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3230.17305301958767305No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3210.17198148386267198No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3190.1709099481376709No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3140.16823110882516823No Hit
ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT3110.16662380523766662RNA PCR Primer, Index 26 (95% over 23bp)
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3010.16126612661266126No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2970.15912305516265912No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.1559084479876559No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA2800.15001500150015No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT2750.14733616218764733No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2690.14412155501264412No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC2690.14412155501264412No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT2650.14197848356264198No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG2530.13554926921263555No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2480.13287042990013287No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG2400.12858428700012858No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2390.12804851913762805No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG2370.12697698341262698No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG2280.12215507265012214No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC2260.1210835369251211No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG2250.12054776906262055No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA2210.1184046976126184No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT2210.1184046976126184No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2190.11733316188761733No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2190.11733316188761733No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2160.11572585830011572No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA2080.11143971540011144No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG2050.10983241181260982No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG2050.10983241181260982No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2030.10876087608760875No Hit
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA2030.10876087608760875No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA2020.10822510822510822No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC2020.10822510822510822No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC1970.10554626891260556No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG1960.10501050105010501No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG1960.10501050105010501No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC1960.10501050105010501No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1930.10340319746260342No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA1920.10286742960010287No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1880.10072435815010072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAATA200.001837987837.022
CTATACA251.2296665E-437.01
ATTACGG403.8038706E-837.02
TCGCCGA200.001837987837.01
GATTACG403.8038706E-837.01
GCCGAGC200.001837987837.03
TACTATA251.2296665E-437.02
TTACGGG355.597667E-737.03
CGCCGAG200.001837987837.02
GTTGTAT200.001837987837.01
ACTATAT251.2296665E-437.03
CTTATAC16150.036.083591
CGTCTTC3500.034.35714337
TTATACA17450.033.3954162
TGATACA352.377679E-531.7142872
TATACAC18650.031.2466493
GCCTCAA303.5880352E-430.83333428
ATACTAT303.5880352E-430.8333341
ACAAATG250.005484698329.612
TAGGACC250.005484698329.64
Produced by FastQC (version 0.11.5)