##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1631033.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 186648 Sequences flagged as poor quality 0 Sequence length 43 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.202263083451204 31.0 31.0 34.0 28.0 34.0 2 31.260559984569884 31.0 31.0 34.0 28.0 34.0 3 30.674028117097425 31.0 31.0 34.0 26.0 34.0 4 34.82020166302345 37.0 35.0 37.0 32.0 37.0 5 35.07113925678282 35.0 35.0 37.0 32.0 37.0 6 35.26282628262826 37.0 35.0 37.0 32.0 37.0 7 35.25862050490763 37.0 35.0 37.0 32.0 37.0 8 35.299274570314175 37.0 35.0 37.0 32.0 37.0 9 37.08689618961896 39.0 37.0 39.0 33.0 39.0 10 36.9231869615533 39.0 37.0 39.0 33.0 39.0 11 36.96391603446059 39.0 37.0 39.0 33.0 39.0 12 36.83584608460846 39.0 37.0 39.0 32.0 39.0 13 36.946305344820196 39.0 37.0 39.0 33.0 39.0 14 37.74088658865887 39.0 37.0 41.0 32.0 41.0 15 37.73553962539111 39.0 37.0 41.0 32.0 41.0 16 37.73277506322061 39.0 37.0 41.0 32.0 41.0 17 37.70701534439158 39.0 37.0 41.0 32.0 41.0 18 37.89910955381252 40.0 37.0 41.0 33.0 41.0 19 37.85671424285286 40.0 37.0 41.0 33.0 41.0 20 37.95386502936008 40.0 37.0 41.0 33.0 41.0 21 37.915123655222665 40.0 37.0 41.0 33.0 41.0 22 37.63858171531439 39.0 37.0 41.0 32.0 41.0 23 37.74743367193862 39.0 37.0 41.0 33.0 41.0 24 37.45931378852171 39.0 36.0 41.0 32.0 41.0 25 37.42743560070293 39.0 36.0 41.0 32.0 41.0 26 37.35407290729073 39.0 36.0 41.0 32.0 41.0 27 37.12001735887875 39.0 36.0 40.0 31.0 41.0 28 37.27819210492478 39.0 36.0 41.0 31.0 41.0 29 37.17776777677768 39.0 36.0 40.0 31.0 41.0 30 36.99074193133599 39.0 35.0 40.0 31.0 41.0 31 36.812181218121815 39.0 35.0 40.0 31.0 41.0 32 36.580509836697956 38.0 35.0 40.0 30.0 41.0 33 36.36704384724187 38.0 35.0 40.0 30.0 41.0 34 36.142642835712145 38.0 35.0 40.0 30.0 41.0 35 36.1969911276842 38.0 35.0 40.0 30.0 41.0 36 36.10708392267798 38.0 35.0 40.0 30.0 41.0 37 35.98477347734774 38.0 35.0 40.0 30.0 41.0 38 35.911925121083534 38.0 35.0 40.0 30.0 41.0 39 35.67485319960568 38.0 35.0 40.0 28.0 41.0 40 35.27182718271827 38.0 34.0 40.0 26.0 41.0 41 35.198994899489946 38.0 34.0 40.0 26.0 41.0 42 34.97364022116497 38.0 34.0 40.0 26.0 41.0 43 33.87797351163688 37.0 33.0 40.0 22.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 3.0 10 15.0 11 8.0 12 7.0 13 12.0 14 7.0 15 10.0 16 8.0 17 7.0 18 13.0 19 20.0 20 34.0 21 78.0 22 121.0 23 284.0 24 455.0 25 742.0 26 1295.0 27 1907.0 28 2600.0 29 3609.0 30 4639.0 31 5876.0 32 7214.0 33 9277.0 34 11861.0 35 15540.0 36 20438.0 37 28913.0 38 39708.0 39 31945.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.73273755947023 23.139813981398138 17.411919763404914 26.715528695726714 2 11.76010458188676 20.921735030645923 44.15584415584416 23.162316231623162 3 13.46009600960096 31.294736616518794 37.011915477262015 18.23325189661823 4 8.842848570571343 21.595195233809093 27.895825296815396 41.66613089880416 5 13.135420684925636 36.9963782092495 30.692533539068194 19.175667566756676 6 24.974283142599973 37.64947923363765 15.408147957652908 21.96808966610947 7 21.145150229308644 34.13055591273413 15.247953366765248 29.476340491191976 8 27.25343962967725 29.125948309116627 24.735330675924736 18.885281385281385 9 19.7993013587073 11.269876987698769 14.314109982426814 54.61671167116712 10 15.52119497664052 27.47739059620248 27.039132484677037 29.96228194247996 11 29.53313188461703 19.242638549569243 23.17249582101067 28.05173374480305 12 18.573464489306073 26.778213535639278 20.687068706870686 33.96125326818396 13 35.75821867901075 15.584951352278084 24.666752389524664 23.99007757918649 14 22.82906862114783 25.222879430800223 22.033989113197034 29.914062834854917 15 28.94700184304145 24.257961510436758 17.969654108267967 28.825382538253823 16 30.086044318717587 21.383031160258884 17.21957910076722 31.31134542025631 17 18.345227379880846 34.75526124040976 18.185568556855685 28.713942822853717 18 27.55079079336505 15.711392567828211 23.22125069649822 33.51656594230852 19 23.823453773948824 24.33029017187433 26.290129012901293 25.556127041275555 20 19.81055248381981 18.353799665680853 21.64127126998414 40.1943765805152 21 28.25586130041576 17.19118340405469 25.05893446487506 29.494020830654495 22 19.358900175731858 34.37057991513437 27.008593716514508 19.26192619261926 23 29.826196905404828 18.687583044018687 22.59011615447259 28.8961038961039 24 26.955552698126954 28.393553641078395 26.573014444301574 18.077879216493077 25 24.847306159187347 25.866336633663366 21.672345806009172 27.614011401140115 26 25.688461703313187 21.17836783678368 28.104774763190605 25.02839569671253 27 20.08111525438258 29.60921092109211 27.64722900861515 22.662444815910163 28 18.571321417856073 18.72133641935622 27.745274527452747 34.96206763533497 29 18.106274913205606 21.987913077021986 35.906090609060904 23.9997214007115 30 25.689533239038187 17.653551069392652 29.624748189104626 27.03216750246453 31 22.12774848913463 22.399382795422397 21.01120826368351 34.461660451759464 32 27.453281042389953 20.294886631520296 33.6949766405212 18.556855685568557 33 24.71925764004972 19.32139642535682 30.715035789293214 25.24431014530024 34 17.22172217221722 36.24201705884874 24.01043675796151 22.525824010972524 35 25.703463203463205 19.145664566456645 37.67841069821268 17.472461531867474 36 22.70798508422271 19.717328875744716 22.3024088123098 35.27227722772277 37 21.07175003214607 25.45004500450045 29.23470918520423 24.243495778149242 38 20.379537953795378 17.336912262654835 31.365993742231367 30.917556041318417 39 29.15916591659166 18.914212849856415 24.94963782092495 26.976983412626975 40 17.772491534867775 18.465775148943468 42.327804208992326 21.433929107196434 41 23.93971540011144 21.422677982083922 23.194462303373196 31.443144314431443 42 24.556384209849558 17.556041318417556 36.24416013029874 21.643414341434145 43 24.86016458788736 17.746774677467748 33.46298915605846 23.93007157858643 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 4.5 2 8.0 3 43.0 4 78.0 5 78.0 6 129.0 7 180.0 8 196.5 9 213.0 10 317.0 11 421.0 12 421.0 13 658.5 14 896.0 15 2492.0 16 4088.0 17 4144.0 18 4200.0 19 4200.0 20 3398.0 21 2596.0 22 2007.5 23 1419.0 24 1368.5 25 1318.0 26 1318.0 27 1350.0 28 1382.0 29 1268.0 30 1154.0 31 1169.0 32 1184.0 33 1184.0 34 1439.5 35 1695.0 36 1713.5 37 1732.0 38 1928.5 39 2125.0 40 2125.0 41 2633.0 42 3141.0 43 4368.0 44 5595.0 45 7807.0 46 10019.0 47 10019.0 48 12784.0 49 15549.0 50 16371.5 51 17194.0 52 29773.5 53 42353.0 54 42353.0 55 29001.0 56 15649.0 57 21656.5 58 27664.0 59 21241.5 60 14819.0 61 14819.0 62 9604.0 63 4389.0 64 3476.5 65 2564.0 66 2091.0 67 1618.0 68 1618.0 69 1299.5 70 981.0 71 649.5 72 318.0 73 187.5 74 57.0 75 57.0 76 39.0 77 21.0 78 16.5 79 12.0 80 12.0 81 12.0 82 12.0 83 7.5 84 3.0 85 1.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 186648.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.845098795593845 #Duplication Level Percentage of deduplicated Percentage of total 1 71.72911809859556 13.517423170888518 2 10.615795758230512 4.0011143971540015 3 4.511855347700005 2.5507907933650507 4 2.7264456700972306 2.055205520552055 5 1.6830613521350999 1.5858728730015859 6 1.3106271677943937 1.481933907676482 7 0.9495650196167623 1.2526252625262526 8 0.6510490703360436 0.9815267241009815 9 0.5970318985614375 1.0126012601260126 >10 4.221868425541593 16.222515108653724 >50 0.5856598623983624 7.701127255582701 >100 0.3525331210553249 12.698234109125197 >500 0.03127309944845624 4.468839741116969 >1k 0.02274407232615 9.154129698684155 >5k 0.0056860180815375 8.087951652308089 >10k+ 0.0056860180815375 13.228108525138227 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT 13360 7.157858643007158 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG 11330 6.07024988213107 No Hit TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 9341 5.0046076036175045 RNA PCR Primer, Index 26 (100% over 22bp) ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC 5755 3.083344048690583 No Hit CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC 3537 1.8950109296643949 RNA PCR Primer, Index 26 (100% over 23bp) CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC 3149 1.6871329990141872 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 2783 1.4910419613389911 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 2618 1.4026402640264026 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA 1542 0.8261540439758263 No Hit CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG 1293 0.6927478462131927 No Hit TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA 1154 0.6182761133256183 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1010 0.5411255411255411 No Hit TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTAT 969 0.5191590587630192 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA 938 0.5025502550255025 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA 880 0.4714757190004715 No Hit TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT 856 0.4586172903004586 No Hit ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 757 0.4055762719129056 RNA PCR Primer, Index 26 (95% over 21bp) GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT 709 0.37985941451287986 No Hit CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC 693 0.3712871287128713 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG 692 0.37075136085037075 No Hit GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT 675 0.36164330718786164 No Hit TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC 602 0.32253225322532253 No Hit GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG 570 0.3053876816253054 No Hit GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG 467 0.2502035917877502 No Hit GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC 443 0.23734516308773734 No Hit CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG 427 0.2287728772877288 No Hit GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG 423 0.22662980583772666 No Hit GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG 398 0.21323560927521326 No Hit GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT 396 0.21216407355021216 No Hit GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG 384 0.20573485920020573 No Hit GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG 373 0.19984141271269987 No Hit GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG 354 0.18966182332518966 No Hit GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG 347 0.1859114482876859 No Hit GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG 343 0.18376837683768377 No Hit TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG 323 0.17305301958767305 No Hit GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG 321 0.17198148386267198 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG 319 0.1709099481376709 No Hit GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA 314 0.16823110882516823 No Hit ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT 311 0.16662380523766662 RNA PCR Primer, Index 26 (95% over 23bp) ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA 301 0.16126612661266126 No Hit GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA 297 0.15912305516265912 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 291 0.1559084479876559 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA 280 0.15001500150015 No Hit CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT 275 0.14733616218764733 No Hit ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG 269 0.14412155501264412 No Hit GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC 269 0.14412155501264412 No Hit AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT 265 0.14197848356264198 No Hit CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG 253 0.13554926921263555 No Hit GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC 248 0.13287042990013287 No Hit CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG 240 0.12858428700012858 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 239 0.12804851913762805 No Hit CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG 237 0.12697698341262698 No Hit GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG 228 0.12215507265012214 No Hit ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC 226 0.1210835369251211 No Hit GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG 225 0.12054776906262055 No Hit GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA 221 0.1184046976126184 No Hit GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT 221 0.1184046976126184 No Hit ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA 219 0.11733316188761733 No Hit CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC 219 0.11733316188761733 No Hit GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC 216 0.11572585830011572 No Hit GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA 208 0.11143971540011144 No Hit ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG 205 0.10983241181260982 No Hit GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG 205 0.10983241181260982 No Hit GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG 203 0.10876087608760875 No Hit CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA 203 0.10876087608760875 No Hit GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA 202 0.10822510822510822 No Hit TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC 202 0.10822510822510822 No Hit GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC 197 0.10554626891260556 No Hit GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG 196 0.10501050105010501 No Hit GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG 196 0.10501050105010501 No Hit ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC 196 0.10501050105010501 No Hit GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC 193 0.10340319746260342 No Hit TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA 192 0.10286742960010287 No Hit GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA 188 0.10072435815010072 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.357678625005358E-4 0.0 5 0.0 0.0 0.0 5.357678625005358E-4 0.0 6 0.0 0.0 0.0 5.357678625005358E-4 0.0 7 0.0 0.0 0.0 5.357678625005358E-4 0.0 8 0.0 0.0 0.0 5.357678625005358E-4 0.0 9 0.0 0.0 0.0 0.0010715357250010715 0.0 10 0.0 0.0 0.0 0.0010715357250010715 0.0 11 0.0 0.0 0.0 0.0010715357250010715 0.0 12 0.0 0.0 0.0 0.0010715357250010715 0.0 13 0.0 0.0 0.0 0.0010715357250010715 0.0 14 0.0 0.0 0.0 0.0010715357250010715 0.0 15 0.0 0.0 0.0 0.0016073035875016073 0.0 16 0.0 0.0 0.0 0.0016073035875016073 0.0 17 0.0 0.0 0.0 0.002143071450002143 0.0 18 0.0 0.0 0.0 0.002143071450002143 0.0 19 0.0 0.0 0.0 0.002143071450002143 0.0 20 0.0 0.0 0.0 0.002678839312502679 0.0 21 0.0 0.0 0.0 0.004821910762504822 0.0 22 0.0 0.0 0.0 0.005893446487505893 0.0 23 0.0 0.0 0.0 0.007500750075007501 0.0 24 0.0 0.0 0.0 0.008036517937508036 0.0 25 0.0 0.0 0.0 0.008572285800008572 0.0 26 0.0 0.0 0.0 0.009643821525009644 0.0 27 0.0 0.0 0.0 0.010715357250010715 0.0 28 0.0 0.0 0.0 0.027324160987527324 0.0 29 0.0 0.0 0.0 0.06375637563756376 0.0 30 0.0 0.0 0.0 0.11304701898761305 0.0 31 0.0 0.0 0.0 0.17305301958767305 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAAATA 20 0.0018379878 37.0 22 CTATACA 25 1.2296665E-4 37.0 1 ATTACGG 40 3.8038706E-8 37.0 2 TCGCCGA 20 0.0018379878 37.0 1 GATTACG 40 3.8038706E-8 37.0 1 GCCGAGC 20 0.0018379878 37.0 3 TACTATA 25 1.2296665E-4 37.0 2 TTACGGG 35 5.597667E-7 37.0 3 CGCCGAG 20 0.0018379878 37.0 2 GTTGTAT 20 0.0018379878 37.0 1 ACTATAT 25 1.2296665E-4 37.0 3 CTTATAC 1615 0.0 36.08359 1 CGTCTTC 350 0.0 34.357143 37 TTATACA 1745 0.0 33.395416 2 TGATACA 35 2.377679E-5 31.714287 2 TATACAC 1865 0.0 31.246649 3 GCCTCAA 30 3.5880352E-4 30.833334 28 ATACTAT 30 3.5880352E-4 30.833334 1 ACAAATG 25 0.0054846983 29.6 12 TAGGACC 25 0.0054846983 29.6 4 >>END_MODULE