FastQCFastQC Report
Fri 10 Feb 2017
ERR1631032.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631032.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323329
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG72612.245700200105775No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT66682.0622956802513848TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC40811.2621818642930267No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT26460.8183614831951356No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC23280.7200096496138608RNA PCR Primer, Index 12 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC20810.6436168732158266No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11120.3439221350389232No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG11000.34021074509245997No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10000.3092824955386No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT5580.17257963251053882No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT5400.167012547590844No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA5240.16206402766222638No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC4340.1342286030637524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4250.131445060603905No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT3800.117527348304668No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG3490.1079395909429714No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA3460.10701174345635558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC3250.034.1538471
CGTCTTC4000.032.837537
GACGTCT250.005490574529.625
GGTATCA1400.027.7500021
TCGCCGA358.8555546E-426.4285721
GTATCAA5000.026.2700021
CCGTCTT11200.023.95089337
TTATACA4750.023.7578952
GCCGTCT11500.023.16521836
ATACTGC400.001928202323.1250026
GCCGAGC400.001928202323.1250023
TATACAC5700.022.7192973
TTTATCC502.6962857E-422.23
TACACAT13850.020.5703962
TCTTGGA450.003819952720.5555532
CGCCGAG450.003819952720.5555532
ATACACA14000.020.2178571
ACGTGGG555.132112E-420.18181836
CGTGGGA656.882538E-519.92307737
TGCCGTC14250.018.69473635
Produced by FastQC (version 0.11.5)