##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631032.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	323329
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.01286305898945	31.0	30.0	31.0	26.0	34.0
2	29.990195744891427	31.0	30.0	31.0	26.0	34.0
3	29.431987232818585	31.0	30.0	31.0	25.0	34.0
4	33.67035743778009	35.0	33.0	37.0	28.0	37.0
5	33.16688574176767	35.0	32.0	37.0	27.0	37.0
6	33.50060155445382	35.0	33.0	37.0	28.0	37.0
7	33.60704421811839	35.0	33.0	37.0	28.0	37.0
8	33.691320605327704	35.0	33.0	37.0	29.0	37.0
9	35.25306730914951	37.0	34.0	39.0	30.0	39.0
10	34.83398643486974	37.0	33.0	39.0	29.0	39.0
11	35.070185476712574	37.0	34.0	39.0	29.0	39.0
12	34.93864453853505	37.0	33.0	39.0	29.0	39.0
13	35.158680477161035	37.0	34.0	39.0	30.0	39.0
14	35.90083784628042	38.0	34.0	40.0	29.0	40.0
15	35.901963634564176	38.0	34.0	40.0	29.0	40.0
16	35.90786474457905	38.0	34.0	40.0	29.0	40.0
17	35.7661422266484	38.0	34.0	40.0	29.0	40.0
18	35.88627682639046	38.0	34.0	40.0	29.0	41.0
19	35.91084004218613	38.0	34.0	40.0	29.0	41.0
20	35.90311725827253	38.0	34.0	40.0	29.0	41.0
21	35.82323268249987	38.0	34.0	40.0	29.0	41.0
22	35.552959988123554	38.0	34.0	40.0	27.0	40.0
23	35.646833411169425	38.0	34.0	40.0	29.0	40.0
24	35.48148789622954	38.0	34.0	40.0	27.0	40.0
25	35.40078062901874	38.0	34.0	39.0	27.0	40.0
26	35.23160001113417	37.0	33.0	39.0	27.0	40.0
27	35.06140185383928	37.0	33.0	39.0	27.0	40.0
28	35.03309322702263	37.0	33.0	39.0	27.0	40.0
29	34.87233746431653	37.0	33.0	39.0	27.0	40.0
30	34.76773193867547	37.0	33.0	39.0	26.0	40.0
31	34.79107658143872	37.0	33.0	39.0	26.0	40.0
32	34.52878646827226	37.0	33.0	39.0	25.0	40.0
33	34.49473755833841	37.0	33.0	39.0	25.0	40.0
34	34.417262293205994	37.0	33.0	39.0	25.0	40.0
35	34.33923650523151	37.0	32.0	39.0	25.0	40.0
36	34.41641176634326	37.0	33.0	39.0	25.0	40.0
37	34.372518394576424	37.0	33.0	39.0	25.0	40.0
38	34.30947734351079	37.0	32.0	39.0	25.0	40.0
39	34.20230786598171	37.0	32.0	39.0	25.0	40.0
40	33.99731852076368	37.0	32.0	39.0	24.0	40.0
41	33.999211329636374	37.0	32.0	39.0	24.0	40.0
42	33.93197022228133	36.0	32.0	39.0	24.0	40.0
43	33.101194139715275	36.0	30.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	5.0
11	9.0
12	11.0
13	7.0
14	6.0
15	13.0
16	19.0
17	25.0
18	62.0
19	111.0
20	222.0
21	349.0
22	656.0
23	1149.0
24	1821.0
25	2818.0
26	4157.0
27	5950.0
28	8015.0
29	10995.0
30	14374.0
31	17895.0
32	22873.0
33	28593.0
34	34802.0
35	40768.0
36	45387.0
37	42340.0
38	30552.0
39	9339.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.35160780505306	25.865604384388657	16.99754738981038	21.785240420747908
2	14.928138212161606	24.274964509833637	38.81433462510323	21.98256265290153
3	16.674347181972543	30.05638219893669	34.07055970853218	19.198710910558596
4	12.587179003429943	19.609747347129396	36.545747520327595	31.25732612911307
5	11.621599052358434	40.48291368853397	34.46396704285728	13.43152021625032
6	26.484787940456933	40.332911678197746	17.885497434501698	15.296802946843618
7	22.20895743963579	36.01285378051459	22.903915207111023	18.874273572738602
8	24.47568884943819	35.26964794373533	23.102165286751266	17.15249792007522
9	22.693293827649235	15.768149470044444	19.501498473690884	42.037058228615436
10	13.799257103445717	28.75584930519687	34.164272304680374	23.28062128667704
11	30.15628044499565	23.8676394632093	24.465482527085413	21.51059756470963
12	19.046543922753603	28.712240473325927	29.001110324158986	23.24010527976148
13	29.191628341410762	21.131417225179305	26.168701230016485	23.508253203393448
14	21.389049543962958	24.420945847727857	26.442725521063686	27.747279087245495
15	23.694131983212145	30.692267009764045	22.835254493101452	22.77834651392235
16	23.65794593123413	28.903995620559865	23.26917783434211	24.168880613863898
17	19.83676069885473	31.96774802136523	25.11806859267186	23.07742268710818
18	22.408753931753726	26.5237575348948	25.922821646063298	25.14466688728818
19	24.984458554599183	28.853582573787072	26.235196966557282	19.92676190505646
20	22.225349411899334	26.7569565365309	26.263960238642376	24.75373381292739
21	26.669429590293483	26.54107735464496	26.008183614831953	20.781309440229613
22	19.484797218931803	31.815581033560242	28.192027315830003	20.50759443167795
23	23.947125064562723	28.544918643239548	25.926223753514222	21.581732538683507
24	24.725589105833375	28.867500286086305	26.966959351001613	19.439951257078704
25	20.898218223543203	28.22512054285264	28.17068682363784	22.70597440996632
26	25.259101410637463	29.065441083231015	25.907976086277447	19.76748141985408
27	19.97748423432479	29.25564981798725	28.481825014149674	22.285040933538284
28	22.15761654537638	28.130170816722288	28.59966164494988	21.11255099295145
29	22.426383033999425	26.811699538241236	29.197504708825996	21.564412718933347
30	19.55624147540122	29.366682233885612	29.844523689492746	21.232552601220426
31	24.573422118028386	27.255829201834665	26.340662297535943	21.830086382601007
32	21.820807907734846	27.46150206136783	31.29691428854201	19.42077574235531
33	19.73222321536268	29.938545568136483	30.137104930272262	20.19212628622858
34	21.327193044855242	30.464635093047637	27.116652078842296	21.091519783254828
35	20.60718339524138	26.45695251585846	33.42354072786542	19.512323361034735
36	22.494425183017917	27.739237742361496	25.703231074230892	24.063106000389695
37	21.83472562003408	26.266125216111142	29.18977264643753	22.709376517417244
38	21.776271228377286	25.873336446777124	28.190171620856773	24.160220703988816
39	24.583628440381162	26.64932622808347	27.341191170603317	21.425854160932055
40	20.35078820643988	25.308277327428097	32.65095305401	21.689981412122016
41	21.94173736349044	27.71511370770948	26.9468559887916	23.396292940008475
42	21.016673419334488	25.620961930417625	31.743518212099747	21.61884643814814
43	22.53988970986209	25.187038589176968	29.568952985967854	22.704118714993086
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	8.5
2	13.0
3	31.0
4	49.0
5	49.0
6	71.5
7	94.0
8	101.5
9	109.0
10	177.5
11	246.0
12	246.0
13	384.5
14	523.0
15	1232.5
16	1942.0
17	2335.0
18	2728.0
19	2728.0
20	2961.5
21	3195.0
22	3885.5
23	4576.0
24	5769.0
25	6962.0
26	6962.0
27	8487.0
28	10012.0
29	12084.0
30	14156.0
31	15337.0
32	16518.0
33	16518.0
34	17667.5
35	18817.0
36	19532.0
37	20247.0
38	21412.5
39	22578.0
40	22578.0
41	23145.0
42	23712.0
43	22426.5
44	21141.0
45	21080.5
46	21020.0
47	21020.0
48	21131.0
49	21242.0
50	25586.0
51	29930.0
52	24819.0
53	19708.0
54	19708.0
55	22757.0
56	25806.0
57	21193.0
58	16580.0
59	12026.0
60	7472.0
61	7472.0
62	6303.0
63	5134.0
64	4249.0
65	3364.0
66	2805.0
67	2246.0
68	2246.0
69	1775.0
70	1304.0
71	1090.5
72	877.0
73	695.5
74	514.0
75	514.0
76	396.0
77	278.0
78	212.5
79	147.0
80	94.5
81	42.0
82	42.0
83	35.0
84	28.0
85	18.0
86	8.0
87	6.0
88	4.0
89	4.0
90	3.5
91	3.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	323329.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.46555420305274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.20896117231173	54.218126841317016
2	7.007087604156831	8.613890458776813
3	1.7348241754890248	3.198957881738608
4	0.7970521451310142	1.9596500731683926
5	0.43492735094860946	1.336652533206095
6	0.3103047609477365	1.1443832462099055
7	0.22070008286123483	0.9495817034007823
8	0.16466640807020685	0.8097049624529041
9	0.13181903233516415	0.7292096889291895
>10	0.8578181808792549	10.285263114851979
>50	0.08906686402993359	3.6509332909391565
>100	0.036733791379577066	3.855996724681328
>500	0.0020128104865521678	0.8109748465640845
>1k	0.0030192157298282517	4.128486747497102
>5k	0.0010064052432760839	4.308187886266642
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG	7261	2.245700200105775	No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	6668	2.0622956802513848	TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC	4081	1.2621818642930267	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT	2646	0.8183614831951356	No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC	2328	0.7200096496138608	RNA PCR Primer, Index 12 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC	2081	0.6436168732158266	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1112	0.3439221350389232	No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG	1100	0.34021074509245997	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1000	0.3092824955386	No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT	558	0.17257963251053882	No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT	540	0.167012547590844	No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA	524	0.16206402766222638	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	434	0.1342286030637524	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	425	0.131445060603905	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	380	0.117527348304668	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	349	0.1079395909429714	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	346	0.10701174345635558	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.092824955386E-4	0.0	0.0	0.0	0.0
2	3.092824955386E-4	0.0	0.0	0.0	0.0
3	3.092824955386E-4	0.0	0.0	0.0	0.0
4	3.092824955386E-4	0.0	0.0	3.092824955386E-4	0.0
5	3.092824955386E-4	0.0	0.0	3.092824955386E-4	0.0
6	3.092824955386E-4	0.0	0.0	3.092824955386E-4	0.0
7	3.092824955386E-4	0.0	0.0	3.092824955386E-4	0.0
8	3.092824955386E-4	0.0	0.0	3.092824955386E-4	0.0
9	3.092824955386E-4	0.0	0.0	3.092824955386E-4	0.0
10	3.092824955386E-4	0.0	0.0	6.185649910772E-4	0.0
11	3.092824955386E-4	0.0	0.0	6.185649910772E-4	0.0
12	3.092824955386E-4	0.0	0.0	6.185649910772E-4	0.0
13	3.092824955386E-4	0.0	0.0	6.185649910772E-4	0.0
14	3.092824955386E-4	0.0	0.0	6.185649910772E-4	0.0
15	3.092824955386E-4	0.0	0.0	6.185649910772E-4	0.0
16	3.092824955386E-4	0.0	0.0	6.185649910772E-4	0.0
17	3.092824955386E-4	0.0	0.0	6.185649910772E-4	0.0
18	3.092824955386E-4	0.0	0.0	6.185649910772E-4	0.0
19	3.092824955386E-4	0.0	0.0	6.185649910772E-4	0.0
20	3.092824955386E-4	0.0	0.0	6.185649910772E-4	0.0
21	3.092824955386E-4	0.0	0.0	6.185649910772E-4	0.0
22	3.092824955386E-4	0.0	0.0	6.185649910772E-4	0.0
23	3.092824955386E-4	0.0	0.0	9.278474866158E-4	0.0
24	3.092824955386E-4	0.0	0.0	9.278474866158E-4	0.0
25	3.092824955386E-4	0.0	0.0	0.0012371299821544	0.0
26	3.092824955386E-4	0.0	0.0	0.0012371299821544	0.0
27	3.092824955386E-4	0.0	0.0	0.0018556949732316	0.0
28	3.092824955386E-4	0.0	0.0	0.0043299549375404	0.0
29	3.092824955386E-4	0.0	0.0	0.007732062388465	0.0
30	3.092824955386E-4	0.0	0.0	0.0132991473081598	0.0
31	3.092824955386E-4	0.0	0.0	0.0334025095181688	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	325	0.0	34.153847	1
CGTCTTC	400	0.0	32.8375	37
GACGTCT	25	0.0054905745	29.6	25
GGTATCA	140	0.0	27.750002	1
TCGCCGA	35	8.8555546E-4	26.428572	1
GTATCAA	500	0.0	26.270002	1
CCGTCTT	1120	0.0	23.950893	37
TTATACA	475	0.0	23.757895	2
GCCGTCT	1150	0.0	23.165218	36
ATACTGC	40	0.0019282023	23.125002	6
GCCGAGC	40	0.0019282023	23.125002	3
TATACAC	570	0.0	22.719297	3
TTTATCC	50	2.6962857E-4	22.2	3
TACACAT	1385	0.0	20.570396	2
TCTTGGA	45	0.0038199527	20.555553	2
CGCCGAG	45	0.0038199527	20.555553	2
ATACACA	1400	0.0	20.217857	1
ACGTGGG	55	5.132112E-4	20.181818	36
CGTGGGA	65	6.882538E-5	19.923077	37
TGCCGTC	1425	0.0	18.694736	35
>>END_MODULE