FastQCFastQC Report
Fri 10 Feb 2017
ERR1631031.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631031.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787641
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT180922.296985555602108No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG139261.7680643846625557No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT126601.6073312587841413RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC74200.9420535497771193No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC49010.6222377961533238RNA PCR Primer, Index 17 (95% over 22bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC38750.49197540503858994No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24260.30800834390286946No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23310.29594701139224594No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC21540.27347484450403164No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG17520.22243636377486697No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA14330.18193567881814177No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTAT11260.1429585305996006No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT10120.1284849315868524No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9690.12302559160835966No Hit
GTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCG9280.11782017441956424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8420.10690149446257877No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA8110.10296569122227005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC21800.033.520641
CGTCTTC6550.031.35114337
TTATACA23450.031.2409362
TATACAC24500.030.2040823
GTCGTAG250.00549530529.61
CTCGCGA250.00549530529.621
TCGCGAG250.00549530529.622
TAGGACC950.027.2631594
GGTATCA3650.025.8493161
CCGTCTT19850.025.35012637
GCCGTCT20500.024.63658536
GTATCAA11000.023.5454561
TTACGGG551.9016737E-523.5454543
CGTGATA400.001930650223.12500227
CGGATCG502.7010552E-422.237
GATTACG603.7243703E-521.5833321
TTACCGG450.003824754220.55555731
TACCGGG450.003824754220.55555732
TGCCGTC25000.020.12835
ATGCCGT25850.019.4661534
Produced by FastQC (version 0.11.5)