##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631031.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	787641
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.473789454840468	31.0	31.0	34.0	28.0	34.0
2	31.489268588100416	31.0	31.0	34.0	28.0	34.0
3	30.962593364235737	31.0	31.0	34.0	27.0	34.0
4	35.09637131637383	37.0	35.0	37.0	32.0	37.0
5	35.29586829532744	37.0	35.0	37.0	33.0	37.0
6	35.482514241894464	37.0	35.0	37.0	33.0	37.0
7	35.482082573151985	37.0	35.0	37.0	33.0	37.0
8	35.50508289944277	37.0	35.0	37.0	33.0	37.0
9	37.28059483952715	39.0	37.0	39.0	34.0	39.0
10	37.12373022734977	39.0	37.0	39.0	33.0	39.0
11	37.18414353747456	39.0	37.0	39.0	33.0	39.0
12	37.11363298761746	39.0	37.0	39.0	33.0	39.0
13	37.20286526475895	39.0	37.0	39.0	33.0	39.0
14	38.32253526670146	40.0	38.0	41.0	34.0	41.0
15	38.339362729974695	40.0	38.0	41.0	34.0	41.0
16	38.333514888127965	40.0	38.0	41.0	34.0	41.0
17	38.28786591861013	40.0	38.0	41.0	33.0	41.0
18	38.32913218077779	40.0	38.0	41.0	34.0	41.0
19	38.331924061850515	40.0	38.0	41.0	34.0	41.0
20	38.332952449148785	40.0	38.0	41.0	34.0	41.0
21	38.291264675150224	40.0	38.0	41.0	34.0	41.0
22	38.21436034944854	40.0	38.0	41.0	34.0	41.0
23	38.22468358046369	40.0	38.0	41.0	34.0	41.0
24	38.168660595372764	40.0	37.0	41.0	33.0	41.0
25	38.132512147031456	40.0	37.0	41.0	33.0	41.0
26	37.98874233311877	40.0	37.0	41.0	33.0	41.0
27	37.92200634553051	40.0	37.0	41.0	33.0	41.0
28	37.911752943282536	40.0	37.0	41.0	33.0	41.0
29	37.8410164021426	40.0	37.0	41.0	33.0	41.0
30	37.74911158763955	40.0	37.0	41.0	33.0	41.0
31	37.7332947370693	40.0	37.0	41.0	33.0	41.0
32	37.64570787960505	40.0	37.0	41.0	33.0	41.0
33	37.59576761494132	40.0	37.0	41.0	32.0	41.0
34	37.54998914480074	40.0	37.0	41.0	32.0	41.0
35	37.491998258089666	39.0	37.0	41.0	32.0	41.0
36	37.47046433590938	39.0	37.0	41.0	32.0	41.0
37	37.416184530769726	39.0	36.0	41.0	32.0	41.0
38	37.38755727545925	39.0	36.0	41.0	32.0	41.0
39	37.29274123617232	39.0	36.0	41.0	31.0	41.0
40	37.19011453187429	39.0	36.0	41.0	31.0	41.0
41	37.1486692541399	39.0	36.0	41.0	31.0	41.0
42	37.11345397205072	39.0	36.0	41.0	31.0	41.0
43	36.44498572319115	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	10.0
11	12.0
12	10.0
13	8.0
14	2.0
15	4.0
16	5.0
17	6.0
18	16.0
19	26.0
20	64.0
21	137.0
22	315.0
23	577.0
24	994.0
25	1781.0
26	2896.0
27	4534.0
28	6856.0
29	9554.0
30	12828.0
31	17541.0
32	22918.0
33	29955.0
34	39854.0
35	53351.0
36	73011.0
37	108407.0
38	186113.0
39	215848.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.549086449283365	21.789749390902706	15.059779772764495	25.601384387049432
2	13.809590917689658	20.894544595824748	41.73563844441821	23.560226042067388
3	14.259669062428188	28.402025796016204	34.17572218815425	23.16258295340136
4	11.572912024640667	19.05792105794391	36.8601939208345	32.50897299658093
5	12.579842847185457	38.053377109622275	35.34719497842291	14.019585064769355
6	28.15838687930161	39.48804087141223	17.31994652386049	15.03362572542567
7	24.7080840128942	32.81126807771561	22.284010101048572	20.196637808341617
8	25.18596670310459	34.89813252484317	21.998473924033917	17.917426848018323
9	23.673983451851797	14.706319249505803	19.0109453418499	42.608751956792496
10	15.05165424349418	28.23100879715505	32.75451633421825	23.962820625132515
11	31.085100953353113	22.643564771260003	25.15409939299757	21.11723488238931
12	20.74853899174878	25.99509167247515	28.210187128399866	25.046182207376205
13	28.976399146311582	19.622264458046242	26.83557610637334	24.56576028926884
14	21.914933326223498	22.108422491972863	25.597067699624574	30.379576482179065
15	24.350306802210653	28.046914774624483	22.364884509567176	25.23789391359769
16	25.591227475461537	26.172075856894196	22.85101969044273	25.385676977201545
17	20.878547460073815	30.742686071446258	24.15910294156856	24.219663526911372
18	24.176750575452523	25.19802803561521	25.068273490079875	25.556947898852396
19	24.357670563111874	28.060880528057837	24.706052630576618	22.87539627825367
20	24.346370998970343	25.37996371443335	24.891543228450523	25.38212205814578
21	26.546611971697764	25.21326340299705	24.432069940493196	23.80805468481199
22	21.823648083327303	30.241061600399167	24.867039679244733	23.068250637028797
23	24.592930027766457	26.23720705245156	24.92137915623996	24.24848376354202
24	25.53752280544055	28.31937392796972	24.328977287876075	21.81412597871365
25	22.9443363156565	29.59533594619884	24.93394833432998	22.52637940381468
26	25.378694100484868	28.93767592088274	25.067003876131384	20.616626102501012
27	23.528993538934618	29.025406244723168	25.020028160037377	22.42557205630484
28	23.060759914732728	25.995345595264848	27.03439765070635	23.909496839296075
29	23.100879715504906	27.207192109095384	25.569644038337263	24.122284137062444
30	24.077339803286016	27.386207675832008	27.02677996701543	21.509672553866547
31	26.67814397676099	25.320037936064782	24.837203751455295	23.164614335718937
32	22.380119876949017	27.278417451605492	26.316430962837128	24.025031708608363
33	21.392741109210924	29.75708476323604	27.390016517677466	21.460157609875566
34	24.19515997770558	28.081702196813012	25.785986255159393	21.937151570322012
35	22.1719031893972	27.37071838566047	29.975331400980902	20.482047023961425
36	23.84233426142113	27.66945854773939	24.14082050071035	24.347386690129134
37	22.25011140862398	26.669510601911277	27.505932271174306	23.57444571829044
38	23.048317698037557	24.307266889356953	27.980133080934085	24.66428233167141
39	24.43346651583653	25.185839741709735	26.18832691543482	24.19236682701891
40	21.615812279960032	23.93755530755763	32.04277075469662	22.40386165778572
41	22.711870001688585	25.123247774049347	26.090566641401345	26.07431558286072
42	23.10722778524734	24.512055619247857	29.495417328452938	22.885299267051867
43	23.42082243052355	23.39124042552381	29.61603065355917	23.571906490393467
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13.0
1	10.5
2	8.0
3	69.0
4	130.0
5	130.0
6	192.5
7	255.0
8	247.0
9	239.0
10	367.0
11	495.0
12	495.0
13	744.5
14	994.0
15	2428.0
16	3862.0
17	4261.0
18	4660.0
19	4660.0
20	4607.0
21	4554.0
22	5464.5
23	6375.0
24	8512.5
25	10650.0
26	10650.0
27	13326.5
28	16003.0
29	18906.5
30	21810.0
31	25620.5
32	29431.0
33	29431.0
34	33635.0
35	37839.0
36	41016.0
37	44193.0
38	47171.0
39	50149.0
40	50149.0
41	51823.0
42	53497.0
43	54532.0
44	55567.0
45	55526.0
46	55485.0
47	55485.0
48	56070.0
49	56655.0
50	70341.0
51	84027.0
52	66030.0
53	48033.0
54	48033.0
55	58125.5
56	68218.0
57	57594.5
58	46971.0
59	35836.0
60	24701.0
61	24701.0
62	22915.5
63	21130.0
64	16695.5
65	12261.0
66	10057.0
67	7853.0
68	7853.0
69	6654.5
70	5456.0
71	4776.5
72	4097.0
73	4909.5
74	5722.0
75	5722.0
76	4997.5
77	4273.0
78	2934.0
79	1595.0
80	928.0
81	261.0
82	261.0
83	180.0
84	99.0
85	78.0
86	57.0
87	36.0
88	15.0
89	15.0
90	10.0
91	5.0
92	4.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	787641.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.65274367434177
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.7070981423738	37.18554818661769
2	12.002255400542644	11.198762894310004
3	3.3299409565699682	4.660526456926536
4	1.3685153365494866	2.55379980841795
5	0.7312891382695091	1.7058322359758844
6	0.4394133549931838	1.2299903170547732
7	0.3109878315308551	1.0155904913173852
8	0.24414050969414294	0.9111859695427204
9	0.18593400165094578	0.7806898187429592
>10	1.3855714639080985	13.070665009916285
>50	0.17941714174459772	5.695233666979307
>100	0.10726902956622662	9.216595965623796
>500	0.004628359143713106	1.4897599889847986
>1k	0.0024503077819657618	2.668587037988799
>5k	2.7225642021841797E-4	0.942451966771865
>10k+	8.16769260655254E-4	5.674780184829298
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT	18092	2.296985555602108	No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG	13926	1.7680643846625557	No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	12660	1.6073312587841413	RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC	7420	0.9420535497771193	No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC	4901	0.6222377961533238	RNA PCR Primer, Index 17 (95% over 22bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC	3875	0.49197540503858994	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2426	0.30800834390286946	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2331	0.29594701139224594	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	2154	0.27347484450403164	No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG	1752	0.22243636377486697	No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA	1433	0.18193567881814177	No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTAT	1126	0.1429585305996006	No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT	1012	0.1284849315868524	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	969	0.12302559160835966	No Hit
GTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCG	928	0.11782017441956424	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	842	0.10690149446257877	No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA	811	0.10296569122227005	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.808841845460051E-4	0.0
5	0.0	0.0	0.0	3.808841845460051E-4	0.0
6	0.0	0.0	0.0	3.808841845460051E-4	0.0
7	0.0	0.0	0.0	3.808841845460051E-4	0.0
8	0.0	0.0	0.0	3.808841845460051E-4	0.0
9	0.0	0.0	0.0	5.078455793946734E-4	0.0
10	0.0	0.0	0.0	5.078455793946734E-4	0.0
11	0.0	0.0	0.0	6.348069742433418E-4	0.0
12	0.0	0.0	0.0	6.348069742433418E-4	0.0
13	0.0	0.0	0.0	6.348069742433418E-4	0.0
14	0.0	0.0	0.0	6.348069742433418E-4	0.0
15	0.0	0.0	0.0	6.348069742433418E-4	0.0
16	0.0	0.0	0.0	6.348069742433418E-4	1.2696139484866836E-4
17	0.0	0.0	0.0	7.617683690920102E-4	1.2696139484866836E-4
18	0.0	0.0	0.0	8.887297639406785E-4	1.2696139484866836E-4
19	0.0	0.0	0.0	8.887297639406785E-4	1.2696139484866836E-4
20	0.0	0.0	0.0	0.0011426525536380153	2.539227896973367E-4
21	0.0	0.0	0.0	0.0015235367381840205	2.539227896973367E-4
22	0.0	0.0	0.0	0.0019044209227300254	2.539227896973367E-4
23	0.0	0.0	0.0	0.0019044209227300254	2.539227896973367E-4
24	0.0	0.0	0.0	0.0021583437124273624	2.539227896973367E-4
25	0.0	0.0	0.0	0.0022853051072760306	2.539227896973367E-4
26	0.0	0.0	0.0	0.0025392278969733673	3.808841845460051E-4
27	0.0	0.0	0.0	0.003047073476368041	5.078455793946734E-4
28	0.0	0.0	0.0	0.009902988798196132	5.078455793946734E-4
29	0.0	0.0	0.0	0.022218244098516964	5.078455793946734E-4
30	0.0	0.0	0.0	0.03796145705975184	5.078455793946734E-4
31	0.0	0.0	0.0	0.07604987551435236	5.078455793946734E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2180	0.0	33.52064	1
CGTCTTC	655	0.0	31.351143	37
TTATACA	2345	0.0	31.240936	2
TATACAC	2450	0.0	30.204082	3
GTCGTAG	25	0.005495305	29.6	1
CTCGCGA	25	0.005495305	29.6	21
TCGCGAG	25	0.005495305	29.6	22
TAGGACC	95	0.0	27.263159	4
GGTATCA	365	0.0	25.849316	1
CCGTCTT	1985	0.0	25.350126	37
GCCGTCT	2050	0.0	24.636585	36
GTATCAA	1100	0.0	23.545456	1
TTACGGG	55	1.9016737E-5	23.545454	3
CGTGATA	40	0.0019306502	23.125002	27
CGGATCG	50	2.7010552E-4	22.2	37
GATTACG	60	3.7243703E-5	21.583332	1
TTACCGG	45	0.0038247542	20.555557	31
TACCGGG	45	0.0038247542	20.555557	32
TGCCGTC	2500	0.0	20.128	35
ATGCCGT	2585	0.0	19.46615	34
>>END_MODULE