FastQCFastQC Report
Fri 10 Feb 2017
ERR1631030.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631030.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853945
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT158741.858901919912875No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG124911.4627405746271716No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT100871.1812236151040172TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC62490.7317801497754539No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC36830.4312924134458308RNA PCR Primer, Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC32210.3771905684792346TruSeq Adapter, Index 11 (95% over 21bp)
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC22320.2613751471113479No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20770.24322409522861543No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC19610.2296400822066995No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT19560.22905456440403069No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA19530.22870325372242942No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC18300.2142995157767772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18010.2109035125212982No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT17870.2092640626738256No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT16460.19275246063856571No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG16310.19099590723055934No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA15460.1810421045851899No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG14770.1729619589083606No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA14700.1721422339846243No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA13410.15703587467576952No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG13130.1537569749808243No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC12490.14626234710666378No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA12380.14497420794079244No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC11970.14017296195890835No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA11890.13923613347463829No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT11880.13911902991410452No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG11830.13853351211143575No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC10560.12366135992364848No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC10280.12038246022870326No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT10030.11745487121535932No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG9940.11640093917055548No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTAT9450.11066286470440133No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG9400.11007734690173254No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA8960.1049247902382472No Hit
ATGCAAAGCAATGTGGCCTCAGAATACACCTCTTAAATTTACA8840.1035195475118421No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT8660.10141168342223444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC18200.033.137361
TTATACA19450.031.3881742
TATACAC20200.030.3143543
TCGCCGA454.0066716E-628.7777771
GGTATCA4150.027.6385541
CGTCTTC5800.027.43103437
GTATCAA12200.023.9590171
CCGTCTT15550.023.91318537
CCTCGAT551.9019162E-523.54545435
CGCCGAG551.9019162E-523.5454542
GCCGTCT15800.023.5348136
AATACGC502.7013134E-422.25
CTAGACT450.003825014520.5555554
ATTACGG450.003825014520.5555552
TTACGGG450.003825014520.5555553
CGCCAAA450.003825014520.55555524
CGAATTA656.899472E-519.92307715
TGCCGTC19100.019.56544735
CCATTTG5000.018.8699999
ATGCCGT19850.018.82619734
Produced by FastQC (version 0.11.5)