##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631030.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	853945
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.510501261790864	31.0	31.0	34.0	28.0	34.0
2	31.537842601104288	31.0	31.0	34.0	30.0	34.0
3	30.988006253330134	31.0	31.0	34.0	27.0	34.0
4	35.12247392981983	37.0	35.0	37.0	32.0	37.0
5	35.32410401138247	37.0	35.0	37.0	33.0	37.0
6	35.515817763439095	37.0	35.0	37.0	33.0	37.0
7	35.522743268009066	37.0	35.0	37.0	33.0	37.0
8	35.550003805865714	37.0	35.0	37.0	33.0	37.0
9	37.32804454619443	39.0	37.0	39.0	34.0	39.0
10	37.17661910310383	39.0	37.0	39.0	33.0	39.0
11	37.23015650890866	39.0	37.0	39.0	34.0	39.0
12	37.168350420694544	39.0	37.0	39.0	33.0	39.0
13	37.22886602767157	39.0	37.0	39.0	34.0	39.0
14	38.36834573655212	40.0	38.0	41.0	34.0	41.0
15	38.39180860594066	40.0	38.0	41.0	34.0	41.0
16	38.38355163388743	40.0	38.0	41.0	34.0	41.0
17	38.359955266439876	40.0	38.0	41.0	34.0	41.0
18	38.40511039938169	40.0	38.0	41.0	34.0	41.0
19	38.432447054552696	40.0	38.0	41.0	34.0	41.0
20	38.40390189063699	40.0	38.0	41.0	34.0	41.0
21	38.35078020247206	40.0	38.0	41.0	34.0	41.0
22	38.28189520402368	40.0	38.0	41.0	34.0	41.0
23	38.299162124024384	40.0	38.0	41.0	34.0	41.0
24	38.24856284655335	40.0	38.0	41.0	34.0	41.0
25	38.21405476933526	40.0	38.0	41.0	34.0	41.0
26	38.138935177324065	40.0	38.0	41.0	33.0	41.0
27	38.04405201740159	40.0	37.0	41.0	33.0	41.0
28	38.044670324201206	40.0	37.0	41.0	33.0	41.0
29	37.99573625936097	40.0	37.0	41.0	33.0	41.0
30	37.928920480827216	40.0	37.0	41.0	33.0	41.0
31	37.89862227661032	40.0	37.0	41.0	33.0	41.0
32	37.810073248277114	40.0	37.0	41.0	33.0	41.0
33	37.82062076597439	40.0	37.0	41.0	33.0	41.0
34	37.798170842384465	40.0	37.0	41.0	33.0	41.0
35	37.789140986831704	40.0	37.0	41.0	33.0	41.0
36	37.78372377612141	40.0	37.0	41.0	33.0	41.0
37	37.73133164313861	40.0	37.0	41.0	33.0	41.0
38	37.68110358395447	40.0	37.0	41.0	33.0	41.0
39	37.618604242661995	40.0	37.0	41.0	32.0	41.0
40	37.53888013865062	40.0	37.0	41.0	32.0	41.0
41	37.51674873674534	40.0	37.0	41.0	32.0	41.0
42	37.48643765113678	40.0	37.0	41.0	32.0	41.0
43	36.81956097875156	39.0	36.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	8.0
10	8.0
11	10.0
12	18.0
13	10.0
14	6.0
15	14.0
16	11.0
17	14.0
18	15.0
19	30.0
20	63.0
21	122.0
22	241.0
23	483.0
24	965.0
25	1607.0
26	2723.0
27	4293.0
28	6507.0
29	9407.0
30	12958.0
31	17725.0
32	23462.0
33	30721.0
34	40695.0
35	54767.0
36	76850.0
37	114186.0
38	204424.0
39	251599.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.585090374672845	22.863533365731985	15.485891948544694	24.06548431105048
2	13.241953521596825	22.56609032197624	42.182341954107116	22.00961420231982
3	14.325746974336756	28.731124369836465	35.89493468548911	21.048193970337667
4	11.721012477384376	19.758649561739926	37.719759469286664	30.800578491589036
5	11.597936635263395	39.50488614606327	36.302220869025525	12.594956349647813
6	26.64094291786942	40.48797053674417	18.373782854867702	14.49730369051871
7	22.7931541258512	34.62939650680079	23.37714958223305	19.200299785114964
8	23.512638401770605	36.898512199263415	22.385282424512116	17.203566974453857
9	23.102424629220852	16.135582502385983	19.65911153528623	41.102881333106936
10	13.531667730357341	29.950640849235022	34.31520765388872	22.20248376651892
11	29.661277951156105	23.90446691531656	26.445497075338576	19.98875805818876
12	19.4852127478936	27.293912371405654	29.592772368243857	23.62810251245689
13	27.55997166093835	21.49728612498463	28.174296939498443	22.768445274578575
14	20.80403304662478	23.324804290674457	26.806644456024685	29.064518206676077
15	23.388157316923223	29.54639935827249	23.73747723799542	23.327966086808868
16	24.289737629472626	28.684048738501893	23.618500020493123	23.40771361153236
17	20.670769194737364	31.387384433423698	25.058873815058348	22.88297255678059
18	22.75778885057	26.969769715848212	26.399006961806677	23.873434471775116
19	24.78789617598323	27.898635158001976	26.717528646458494	20.595940019556295
20	23.479498094139554	26.525830117864736	26.940845136396373	23.05382665159934
21	23.160742202366663	28.40276598609981	25.47541117987692	22.961080631656607
22	21.452786771981803	31.485751424272056	25.729174595553577	21.332287208192565
23	21.841453489393345	27.249413018402823	26.62255765886562	24.286575833338212
24	24.66622557658866	28.8328873639403	26.788610507702487	19.712276551768557
25	22.191007617586614	29.28022296517926	26.09395218661623	22.434817230617895
26	24.290674457956893	27.23992762999959	26.064090778680125	22.40530713336339
27	22.65239564608962	29.537265280550855	26.63719560393234	21.17314346942719
28	19.69108080731195	28.38695700542775	27.97229329757771	23.94966888968259
29	24.483895332837594	27.451767971005157	26.75617282143464	21.30816387472261
30	21.860424266199814	28.356861390370575	27.23711714454678	22.545597198882835
31	21.640152468835815	28.332035435537417	27.83762420296389	22.190187892662877
32	23.05628582637055	28.88593527686209	27.28442698300242	20.773351913764937
33	20.12986784863194	26.498896298941972	28.748221489674393	24.6230143627517
34	20.06546089033837	29.145553870565433	28.706298415003307	22.082686824092885
35	20.92394709261135	28.7308901627154	29.668772579030268	20.676390165642985
36	22.005164267019538	27.103853292659362	25.38992558068728	25.50105685963382
37	22.15786730995556	27.489006903254893	27.727781063183226	22.62534472360632
38	21.9939223252083	26.4507667355626	28.65020580950764	22.90510512972147
39	23.405605747442753	26.62548524787896	27.149523681267528	22.81938532341076
40	20.827102448049935	25.48278870419055	31.61304299457225	22.077065853187268
41	21.544478859879735	27.617703716281493	26.950916042602273	23.8869013812365
42	22.876648964511766	25.637014093413512	29.65870167282436	21.82763526925036
43	22.04205188858767	25.722851003284752	29.694301155226626	22.540795952900947
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12.0
1	17.0
2	22.0
3	83.0
4	144.0
5	144.0
6	163.5
7	183.0
8	200.5
9	218.0
10	372.5
11	527.0
12	527.0
13	722.5
14	918.0
15	2540.0
16	4162.0
17	4859.5
18	5557.0
19	5557.0
20	6076.0
21	6595.0
22	8751.0
23	10907.0
24	13651.0
25	16395.0
26	16395.0
27	20381.0
28	24367.0
29	31601.0
30	38835.0
31	40012.5
32	41190.0
33	41190.0
34	44554.0
35	47918.0
36	49627.0
37	51336.0
38	56530.5
39	61725.0
40	61725.0
41	65598.5
42	69472.0
43	63035.5
44	56599.0
45	57798.0
46	58997.0
47	58997.0
48	58103.0
49	57209.0
50	59179.0
51	61149.0
52	74839.0
53	88529.0
54	88529.0
55	63963.5
56	39398.0
57	42694.0
58	45990.0
59	37045.0
60	28100.0
61	28100.0
62	21201.5
63	14303.0
64	11682.0
65	9061.0
66	7473.5
67	5886.0
68	5886.0
69	4735.5
70	3585.0
71	2917.0
72	2249.0
73	1712.0
74	1175.0
75	1175.0
76	897.0
77	619.0
78	484.0
79	349.0
80	245.5
81	142.0
82	142.0
83	99.5
84	57.0
85	45.5
86	34.0
87	27.0
88	20.0
89	20.0
90	14.5
91	9.0
92	5.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	853945.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.00517565520493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.23437141975258	37.00972330641329
2	10.665928775649231	9.600439961479971
3	2.7601601958733415	3.7266448335535376
4	1.1701682882166826	2.1065451742936907
5	0.6166607188706776	1.3876461986219895
6	0.38480577256298365	1.0390950832400352
7	0.2958036692253306	0.9318887265058097
8	0.20839674098957991	0.7503145547366631
9	0.16134950703994963	0.6535406615592292
>10	1.1933348015735372	10.608839360653565
>50	0.14731448441676173	4.6769736631700605
>100	0.14077285873175188	13.172320311375534
>500	0.013082979436009959	3.97818039781538
>1k	0.006803149306725178	5.0938420249808205
>5k	2.6165958872019913E-4	0.7358844741563054
>10k+	7.849787661605975E-4	4.528121267444112
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT	15874	1.858901919912875	No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG	12491	1.4627405746271716	No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT	10087	1.1812236151040172	TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC	6249	0.7317801497754539	No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC	3683	0.4312924134458308	RNA PCR Primer, Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC	3221	0.3771905684792346	TruSeq Adapter, Index 11 (95% over 21bp)
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	2232	0.2613751471113479	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2077	0.24322409522861543	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1961	0.2296400822066995	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1956	0.22905456440403069	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1953	0.22870325372242942	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1830	0.2142995157767772	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1801	0.2109035125212982	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1787	0.2092640626738256	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	1646	0.19275246063856571	No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG	1631	0.19099590723055934	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	1546	0.1810421045851899	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1477	0.1729619589083606	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	1470	0.1721422339846243	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1341	0.15703587467576952	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	1313	0.1537569749808243	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	1249	0.14626234710666378	No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA	1238	0.14497420794079244	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1197	0.14017296195890835	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	1189	0.13923613347463829	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	1188	0.13911902991410452	No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG	1183	0.13853351211143575	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	1056	0.12366135992364848	No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC	1028	0.12038246022870326	No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT	1003	0.11745487121535932	No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG	994	0.11640093917055548	No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTAT	945	0.11066286470440133	No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG	940	0.11007734690173254	No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA	896	0.1049247902382472	No Hit
ATGCAAAGCAATGTGGCCTCAGAATACACCTCTTAAATTTACA	884	0.1035195475118421	No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT	866	0.10141168342223444	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.1710356053375803E-4	0.0
3	0.0	0.0	0.0	1.1710356053375803E-4	0.0
4	0.0	0.0	0.0	1.1710356053375803E-4	0.0
5	0.0	0.0	0.0	1.1710356053375803E-4	0.0
6	0.0	0.0	0.0	1.1710356053375803E-4	0.0
7	0.0	0.0	0.0	1.1710356053375803E-4	0.0
8	0.0	0.0	0.0	1.1710356053375803E-4	0.0
9	0.0	0.0	0.0	3.513106816012741E-4	0.0
10	0.0	0.0	0.0	3.513106816012741E-4	0.0
11	0.0	0.0	0.0	3.513106816012741E-4	0.0
12	0.0	0.0	0.0	3.513106816012741E-4	0.0
13	0.0	0.0	0.0	3.513106816012741E-4	0.0
14	0.0	0.0	0.0	3.513106816012741E-4	0.0
15	0.0	0.0	0.0	4.6841424213503214E-4	0.0
16	0.0	0.0	0.0	7.026213632025482E-4	0.0
17	0.0	0.0	0.0	8.197249237363062E-4	0.0
18	0.0	0.0	0.0	9.368284842700643E-4	0.0
19	0.0	0.0	0.0	9.368284842700643E-4	0.0
20	0.0	0.0	0.0	0.0010539320448038222	0.0
21	0.0	0.0	0.0	0.0012881391658713384	0.0
22	0.0	0.0	0.0	0.0019907605290738865	0.0
23	0.0	0.0	0.0	0.0022249676501414025	0.0
24	0.0	0.0	0.0	0.0024591747712089185	0.0
25	0.0	0.0	0.0	0.0026933818922764345	0.0
26	0.0	0.0	0.0	0.002927589013343951	0.0
27	0.0	0.0	0.0	0.003864417497614015	0.0
28	0.0	0.0	0.0	0.008431456358430577	0.0
29	0.0	0.0	0.0	0.018151051882732496	0.0
30	0.0	0.0	0.0	0.03325741119158728	0.0
31	0.0	0.0	0.0	0.07482917518107138	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1820	0.0	33.13736	1
TTATACA	1945	0.0	31.388174	2
TATACAC	2020	0.0	30.314354	3
TCGCCGA	45	4.0066716E-6	28.777777	1
GGTATCA	415	0.0	27.638554	1
CGTCTTC	580	0.0	27.431034	37
GTATCAA	1220	0.0	23.959017	1
CCGTCTT	1555	0.0	23.913185	37
CCTCGAT	55	1.9019162E-5	23.545454	35
CGCCGAG	55	1.9019162E-5	23.545454	2
GCCGTCT	1580	0.0	23.53481	36
AATACGC	50	2.7013134E-4	22.2	5
CTAGACT	45	0.0038250145	20.555555	4
ATTACGG	45	0.0038250145	20.555555	2
TTACGGG	45	0.0038250145	20.555555	3
CGCCAAA	45	0.0038250145	20.555555	24
CGAATTA	65	6.899472E-5	19.923077	15
TGCCGTC	1910	0.0	19.565447	35
CCATTTG	500	0.0	18.869999	9
ATGCCGT	1985	0.0	18.826197	34
>>END_MODULE