FastQCFastQC Report
Fri 10 Feb 2017
ERR1631029.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631029.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184868
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG131627.119674578618257No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT116966.326676331220114No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT116816.318562433736504TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC67453.6485492351299302No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC44612.4130731116255926RNA PCR Primer, Index 11 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC35861.93976242508168No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24921.3479888352770626No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21111.1418958391933705No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG15420.8341086613151005No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA12440.6729125646407166No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12230.6615531081636628No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT11070.5988056342904127No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9190.4971114524958349No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT7960.43057749313023347No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7580.41002228617175496No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7440.40244931518705235No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7200.3894670792132765No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5620.3040006923859186No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG5330.28831382391760607No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5270.2850682649241621No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5060.2737088084471082No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4670.2526126749897224No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG4420.2390895125170392No Hit
ATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT4080.22069801155419003No Hit
TCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT3950.21366596706839475TruSeq Adapter, Index 11 (95% over 23bp)
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3630.19635631910336024No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3620.1958153926044529No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG3280.17742389164160374No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3270.1768829651426964No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3230.1747192591470671No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3110.16822814116017915No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3110.16822814116017915No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG3060.16552350866564253No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG2930.15849146417984725No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2880.1557868316853106No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2880.1557868316853106No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG2840.15362312568968128No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG2840.15362312568968128No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG2740.148213860700608No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG2550.13793625722136876No Hit
CTTTACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA2510.13577255122573945No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA2510.13577255122573945No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2500.13523162472683212No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2400.12982235973775885No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG2400.12982235973775885No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC2340.12657680074431485No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT2300.12441309474868555No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2290.12387216824977822No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2280.12333124175087089No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC2280.12333124175087089No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG2220.12008568275742693No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG2160.11684012376398296No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2120.11467641776835363No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT2090.11305363827163165No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG2040.11034900577709501No Hit
CTGATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT2020.10926715277928035No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2010.10872622628037303No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1990.10764437328255837No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1940.10493974078802173No Hit
TCGCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT1920.10385788779020708TruSeq Adapter, Index 11 (95% over 21bp)
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1900.1027760347923924No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA1890.10223510829348507No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC1890.10223510829348507No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA1880.10169418179457775No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG1880.10169418179457775No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC1850.10007140229785576No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG1850.10007140229785576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTTT308.274277E-637.03
TATGTAG200.001837943437.05
CCCACTC200.001837943437.02
ATTACTT308.274277E-637.02
AGGTGAA200.001837943437.010
TCTGCCG200.001837943437.033
TCGCCGA200.001837943437.01
ATATGTA200.001837943437.04
TGTAGGT200.001837943437.07
ACCCACT200.001837943437.01
CTGCCGT200.001837943437.034
CTTATAC13500.035.7666661
TTTACAC700.034.3571432
TTATACA14750.032.735592
TGGAGCT303.587906E-430.83333429
TGATACA303.587906E-430.8333342
GGAGCTG303.587906E-430.83333430
TATACAC16100.030.5652183
CGTCTTC5600.030.39285737
GGTATCA3050.029.7213121
Produced by FastQC (version 0.11.5)