##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631029.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	184868
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.93834519765454	31.0	30.0	34.0	28.0	34.0
2	30.98839171733345	31.0	31.0	34.0	28.0	34.0
3	30.396250297509575	31.0	30.0	34.0	26.0	34.0
4	34.55195058095506	35.0	35.0	37.0	30.0	37.0
5	34.77552632148344	35.0	35.0	37.0	32.0	37.0
6	34.993297920678536	35.0	35.0	37.0	32.0	37.0
7	34.9700002163706	35.0	35.0	37.0	32.0	37.0
8	35.03225544712984	36.0	35.0	37.0	32.0	37.0
9	36.783905272951515	39.0	37.0	39.0	32.0	39.0
10	36.614671008503365	38.0	35.0	39.0	32.0	39.0
11	36.61129021788519	38.0	35.0	39.0	32.0	39.0
12	36.494715148105676	38.0	35.0	39.0	32.0	39.0
13	36.64080316766558	38.0	35.0	39.0	32.0	39.0
14	37.294556115715	39.0	36.0	40.0	32.0	41.0
15	37.3205963173724	39.0	36.0	40.0	32.0	41.0
16	37.19265638185083	39.0	36.0	40.0	31.0	41.0
17	37.28611225306705	39.0	36.0	40.0	32.0	41.0
18	37.42870588744401	39.0	36.0	40.0	32.0	41.0
19	37.3656446762014	39.0	36.0	40.0	32.0	41.0
20	37.365087521907526	39.0	36.0	40.0	32.0	41.0
21	37.1216814159292	39.0	36.0	40.0	31.0	41.0
22	37.026532444771405	39.0	36.0	40.0	31.0	41.0
23	37.08775991518272	39.0	36.0	40.0	31.0	41.0
24	36.931075145509226	39.0	36.0	40.0	31.0	41.0
25	36.86607200813553	39.0	35.0	40.0	31.0	41.0
26	36.577601315533244	38.0	35.0	40.0	30.0	41.0
27	36.34789146850726	38.0	35.0	40.0	30.0	41.0
28	36.4713363048229	38.0	35.0	40.0	30.0	41.0
29	36.383695393469935	38.0	35.0	40.0	30.0	41.0
30	36.21148062401281	38.0	35.0	40.0	30.0	41.0
31	36.13755760867213	38.0	35.0	40.0	30.0	41.0
32	35.844624272453856	38.0	35.0	40.0	30.0	41.0
33	35.85330073349633	38.0	35.0	40.0	30.0	41.0
34	35.7404959214142	38.0	35.0	40.0	29.0	41.0
35	35.77988618906463	38.0	35.0	40.0	29.0	41.0
36	35.68199472055737	38.0	34.0	40.0	28.0	41.0
37	35.550684812947615	38.0	34.0	40.0	28.0	41.0
38	35.40917844083346	38.0	34.0	40.0	27.0	41.0
39	35.19071986498474	38.0	34.0	40.0	26.0	41.0
40	34.759915182724974	38.0	33.0	40.0	25.0	41.0
41	34.779047752991325	38.0	33.0	40.0	25.0	41.0
42	34.59592249605124	38.0	33.0	40.0	24.0	41.0
43	33.50822208278339	37.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	13.0
10	21.0
11	15.0
12	10.0
13	13.0
14	7.0
15	10.0
16	11.0
17	12.0
18	26.0
19	53.0
20	63.0
21	105.0
22	215.0
23	382.0
24	631.0
25	1057.0
26	1505.0
27	2243.0
28	3302.0
29	4259.0
30	5511.0
31	6822.0
32	8216.0
33	10557.0
34	13129.0
35	16945.0
36	22237.0
37	30445.0
38	38607.0
39	18445.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.931626890538116	24.764156046476405	18.864270722894172	26.439946340091307
2	11.570958738126663	20.66122855226432	43.23517320466495	24.53263950494407
3	13.285695739662895	31.63770906809183	37.366120691520436	17.71047450072484
4	8.72298072137958	21.200532271674923	26.151091589674795	43.9253954172707
5	12.768029080208581	37.88973754246273	29.87969794664301	19.46253543068568
6	25.3099508838739	36.37243871302767	15.361230715970315	22.956379687128113
7	20.259320163576174	35.92184693943787	14.75863859618755	29.060194300798408
8	27.876106194690266	27.829586515784232	25.454919185581065	18.839388103944437
9	18.637622519852002	11.055455784667979	13.809312590605188	56.497609104874826
10	15.156219572884435	26.126749897223966	27.35681675573923	31.360213774152367
11	28.484107579462105	18.820996602981587	22.227210766601036	30.46768505095528
12	17.179825605296752	28.06705324880455	19.9293549992427	34.82376614665599
13	36.67048921392561	14.857087218988685	23.150031373736937	25.32239219334877
14	23.44483631564143	26.369625895233355	20.641755198303656	29.54378259082156
15	29.251141354912697	25.37702576973841	16.7773762901097	28.594456585239197
16	30.578574983231277	21.133998312309323	16.31380228054612	31.97362442391328
17	17.305320553043252	35.97648051582751	17.3312850249908	29.386913906138435
18	26.554622757859665	15.225458164744573	19.685938074734405	38.53398100266136
19	25.58690525131445	25.338619988315987	19.763290564078154	29.311184196291407
20	25.635047709717202	18.841010883441157	22.54581647445745	32.97812493238419
21	28.894670792132764	19.62210874786334	26.610338187247116	24.872882272756776
22	18.383927991864464	41.64268559188177	20.059177358980463	19.914209057273297
23	24.859900036783003	16.792522232079104	24.964839777571022	33.382737953566874
24	24.111798688794167	28.37970876517299	27.31300170932774	20.19549083670511
25	23.089988532358223	32.03799467728325	20.969015730142587	23.903001060215935
26	29.93865893502391	20.667719670251206	25.82058549884242	23.57303589588247
27	24.361165804790446	35.31817296665729	23.472964493584612	16.847696734967652
28	17.001860787156243	17.58389769998053	35.52697059523552	29.887270917627713
29	17.523313932102905	26.806694506350475	25.782720643918903	29.887270917627713
30	23.084579267369147	21.468290888634055	30.13339247463055	25.31373736936625
31	24.596468831815134	16.55505549905879	34.44133111192851	24.40714455719757
32	20.654196507778522	35.09963866109873	26.262522448449705	17.983642382673043
33	23.160849903715082	19.35056364541186	32.397710799056625	25.09087565181643
34	16.617262046433133	31.534933033299435	30.733820888417686	21.113984031849753
35	18.69712443473181	25.272626955449297	39.91875283986412	16.11149576995478
36	30.362204383668345	19.26185169959106	21.144275915788562	29.231668000952034
37	20.983620745613084	24.003072462513796	29.92621762554904	25.087089166324077
38	20.277170738040116	16.84445117597421	30.47038968344981	32.407988402535864
39	30.627799294631846	18.897808165826426	24.69275374862064	25.78163879092109
40	17.05487158404916	17.553605816041717	43.75608542311271	21.63543717679642
41	23.805093363913713	22.20341000064911	22.9552978341303	31.03619880130688
42	22.888222948265792	17.128437587900557	38.537226561654805	21.44611290217885
43	24.891814700218536	17.313434450526863	33.284830257264645	24.50992059198996
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	3.0
2	6.0
3	34.5
4	63.0
5	63.0
6	105.0
7	147.0
8	166.0
9	185.0
10	291.5
11	398.0
12	398.0
13	626.0
14	854.0
15	2253.5
16	3653.0
17	3632.5
18	3612.0
19	3612.0
20	3015.5
21	2419.0
22	1876.0
23	1333.0
24	1327.5
25	1322.0
26	1322.0
27	1379.5
28	1437.0
29	1362.0
30	1287.0
31	1405.5
32	1524.0
33	1524.0
34	1719.0
35	1914.0
36	2008.0
37	2102.0
38	2259.0
39	2416.0
40	2416.0
41	2937.0
42	3458.0
43	4652.0
44	5846.0
45	7674.5
46	9503.0
47	9503.0
48	13473.5
49	17444.0
50	28440.0
51	39436.0
52	27867.0
53	16298.0
54	16298.0
55	24844.0
56	33390.0
57	26874.0
58	20358.0
59	13133.0
60	5908.0
61	5908.0
62	4791.0
63	3674.0
64	2966.0
65	2258.0
66	1856.0
67	1454.0
68	1454.0
69	1101.5
70	749.0
71	507.5
72	266.0
73	173.0
74	80.0
75	80.0
76	59.0
77	38.0
78	27.0
79	16.0
80	14.0
81	12.0
82	12.0
83	8.5
84	5.0
85	3.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	184868.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.056472726485925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.75743028210799	14.592574161023
2	10.585792113922002	4.246273016422529
3	4.315227358541454	2.5964471947551764
4	2.5459841415394573	2.042538459874072
5	1.6802416527320785	1.684986044096328
6	1.1785964723016344	1.4183092801350152
7	0.8846216085009979	1.2419672414912262
8	0.6904363773666324	1.1078174697622087
9	0.5313123685204164	0.9590626825626934
>10	4.010464426344463	16.27918298461605
>50	0.47197799234047144	6.545210636778674
>100	0.2912778467015481	10.981348854317675
>500	0.024273153891795674	3.2807192158729475
>1k	0.021576136792707264	9.610100179587597
>5k	0.002697017099088408	3.6485492351299302
>10k+	0.008091051297265225	19.764913343574875
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG	13162	7.119674578618257	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT	11696	6.326676331220114	No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	11681	6.318562433736504	TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC	6745	3.6485492351299302	No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC	4461	2.4130731116255926	RNA PCR Primer, Index 11 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC	3586	1.93976242508168	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2492	1.3479888352770626	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2111	1.1418958391933705	No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG	1542	0.8341086613151005	No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA	1244	0.6729125646407166	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1223	0.6615531081636628	No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT	1107	0.5988056342904127	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	919	0.4971114524958349	No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT	796	0.43057749313023347	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	758	0.41002228617175496	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	744	0.40244931518705235	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	720	0.3894670792132765	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	562	0.3040006923859186	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	533	0.28831382391760607	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	527	0.2850682649241621	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	506	0.2737088084471082	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	467	0.2526126749897224	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	442	0.2390895125170392	No Hit
ATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT	408	0.22069801155419003	No Hit
TCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT	395	0.21366596706839475	TruSeq Adapter, Index 11 (95% over 23bp)
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	363	0.19635631910336024	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	362	0.1958153926044529	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	328	0.17742389164160374	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	327	0.1768829651426964	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	323	0.1747192591470671	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	311	0.16822814116017915	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	311	0.16822814116017915	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	306	0.16552350866564253	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	293	0.15849146417984725	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	288	0.1557868316853106	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	288	0.1557868316853106	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	284	0.15362312568968128	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	284	0.15362312568968128	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	274	0.148213860700608	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	255	0.13793625722136876	No Hit
CTTTACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA	251	0.13577255122573945	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	251	0.13577255122573945	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	250	0.13523162472683212	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	240	0.12982235973775885	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	240	0.12982235973775885	No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	234	0.12657680074431485	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	230	0.12441309474868555	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	229	0.12387216824977822	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	228	0.12333124175087089	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	228	0.12333124175087089	No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG	222	0.12008568275742693	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	216	0.11684012376398296	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	212	0.11467641776835363	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	209	0.11305363827163165	No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG	204	0.11034900577709501	No Hit
CTGATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT	202	0.10926715277928035	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	201	0.10872622628037303	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	199	0.10764437328255837	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	194	0.10493974078802173	No Hit
TCGCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	192	0.10385788779020708	TruSeq Adapter, Index 11 (95% over 21bp)
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	190	0.1027760347923924	No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA	189	0.10223510829348507	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	189	0.10223510829348507	No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA	188	0.10169418179457775	No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG	188	0.10169418179457775	No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC	185	0.10007140229785576	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	185	0.10007140229785576	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	5.409264989073285E-4	0.0
11	0.0	0.0	0.0	5.409264989073285E-4	0.0
12	0.0	0.0	0.0	5.409264989073285E-4	0.0
13	0.0	0.0	0.0	5.409264989073285E-4	0.0
14	0.0	0.0	0.0	0.001081852997814657	0.0
15	0.0	0.0	0.0	0.001081852997814657	0.0
16	0.0	0.0	0.0	0.001081852997814657	0.0
17	0.0	0.0	0.0	0.001081852997814657	0.0
18	0.0	0.0	0.0	0.0016227794967219854	0.0
19	0.0	0.0	0.0	0.0016227794967219854	0.0
20	0.0	0.0	0.0	0.0016227794967219854	0.0
21	0.0	0.0	0.0	0.0032455589934439707	0.0
22	0.0	0.0	0.0	0.004327411991258628	0.0
23	0.0	0.0	0.0	0.005950191487980613	0.0
24	0.0	0.0	0.0	0.008654823982517256	0.0
25	0.0	0.0	0.0	0.008654823982517256	0.0
26	0.0	0.0	0.0	0.009195750481424583	0.0
27	0.0	0.0	0.0	0.011900382975961226	0.0
28	0.0	0.0	0.0	0.023800765951922452	0.0
29	0.0	0.0	0.0	0.05463357638964018	0.0
30	0.0	0.0	0.0	0.09358028431096782	0.0
31	0.0	0.0	0.0	0.13631347772464678	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACTTT	30	8.274277E-6	37.0	3
TATGTAG	20	0.0018379434	37.0	5
CCCACTC	20	0.0018379434	37.0	2
ATTACTT	30	8.274277E-6	37.0	2
AGGTGAA	20	0.0018379434	37.0	10
TCTGCCG	20	0.0018379434	37.0	33
TCGCCGA	20	0.0018379434	37.0	1
ATATGTA	20	0.0018379434	37.0	4
TGTAGGT	20	0.0018379434	37.0	7
ACCCACT	20	0.0018379434	37.0	1
CTGCCGT	20	0.0018379434	37.0	34
CTTATAC	1350	0.0	35.766666	1
TTTACAC	70	0.0	34.357143	2
TTATACA	1475	0.0	32.73559	2
TGGAGCT	30	3.587906E-4	30.833334	29
TGATACA	30	3.587906E-4	30.833334	2
GGAGCTG	30	3.587906E-4	30.833334	30
TATACAC	1610	0.0	30.565218	3
CGTCTTC	560	0.0	30.392857	37
GGTATCA	305	0.0	29.721312	1
>>END_MODULE