FastQCFastQC Report
Fri 10 Feb 2017
ERR1631028.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631028.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276503
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT61722.232163846323548No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54131.9576641121434486No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG32861.1884138689272812No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT32281.167437604655284No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA26220.9482718089857977No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC20560.7435724024694126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19310.6983649363659707No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA18900.6835368874840417No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT18890.6831752277552142No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA14950.5406812945971653No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT14570.526938224901719No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT11270.4075905143886323No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT11240.40650553520214966No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC10890.39384744469318594No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC10350.37431781933649905No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC10200.368892923404086No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG10090.3649146663869831No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG9030.3265787351312644No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG8450.30560247085926734No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG8090.29258272062147606No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7440.2690748382476863No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT7340.26545824095941095No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7330.2650965812305834No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG7020.2538851296369298No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7000.25316181017927475No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6870.24846023370451675No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG6860.2480985739756892No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG6680.24158869885679363No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG6610.23905708075500084No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6600.2386954210261733No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6510.2354404834667255No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6500.23507882373789796No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG6210.22459069160189943No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6130.22169741377127916No Hit
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTC5960.21554919838121106Illumina PCR Primer Index 7 (95% over 21bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA5650.20433774678755745No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5490.1985511911263169No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5430.1963812327533517No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5380.194572934109214No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG5370.19421127438038646No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT5290.1913179965497662No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5200.18806305899031836No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG5110.18480812143087055No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4900.1772132671254923No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4840.1750433087525271No Hit
CACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCG4760.1721500309219068No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4560.1649168363453561No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4550.16455517661652858No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4480.16202355851473582No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG4380.15840696122646047No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA4370.15804530149763293No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA4370.15804530149763293No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4350.15732198203997788No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA4270.1544287042093576No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4220.15262040556521989No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC4220.15262040556521989No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4210.15225874583639237No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4090.14791882909046195No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA4060.14683384990397935No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3950.14285559288687644No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG3940.14249393315804892No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3920.14177061370039384No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG3920.14177061370039384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC3870.13996231505625617No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3810.13779235668329096No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG3760.13598405803915328No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA3670.13272912047970548No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3660.13236746075087794No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC3590.12983584264908518No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA3570.1291125231914301No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA3390.12260264807253449No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3340.12079434942839681No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3330.12043268969956927No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3310.11970937024191419No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG3250.11753941186894898No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT3210.11609277295363883No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA3110.11247617566536348No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC3080.11139119647888088No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC3040.10994455756357074No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA3030.1095828978347432No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT3020.10922123810591566No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3000.10849791864826061No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG2980.10777459919060553No Hit
TTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTA2950.10668962000412291No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT2950.10668962000412291No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA2940.10632796027529537No Hit
ATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTG2930.10596630054646786No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC2920.10560464081764032No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA2890.10451966163115771No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2860.10343468244467512No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT2840.10271136298702004No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAACG251.231202E-437.02
GCAACGC251.231202E-437.03
TTACTTA200.001839519837.03
GTTACTT200.001839519837.02
TAGCAAC251.231202E-437.01
TCAGAGT501.7826096E-1037.03
ACTCAGA554.965841E-1033.6363641
CTTATAC4250.032.647061
ATTACTT352.3818002E-531.7142872
TTACTTT352.3818002E-531.7142873
CTCAGAG705.456968E-1231.7142872
TTATACA4450.031.1797732
TATACAC4500.030.8333343
GGGATCA551.845001E-830.2727281
CCTTATT250.005489215729.61
AGATACA250.005489215729.64
CTTATTA250.005489215729.62
CACTACA250.005489215729.621
TGTACTG250.005489215729.62
TTATTAC250.005489215729.63
Produced by FastQC (version 0.11.5)