FastQCFastQC Report
Fri 10 Feb 2017
ERR1631027.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631027.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296135
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT213307.202796022084522No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG181156.117142519459031No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT141884.791058132270755TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC95763.23366032383879No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC52261.764735677984703RNA PCR Primer, Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC48611.6414810812636131No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43501.4689246458540868No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42611.4388707852837388No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG23010.777010485082817No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA21920.7402029479798065No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA18340.6193121380451483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16580.5598797845577186No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT14350.48457629121853213No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA13660.46127610718084655No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT12130.4096104817059787No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11620.39238860654768937No Hit
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT11550.3900248197612575No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT9900.3343069883667922No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9810.3312678339270941No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9370.31640974555523665No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8560.28905735559795365No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG8310.28061525993212555No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC8220.27757610549242745No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6200.20936397251253652No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6170.2083509210326371No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5960.20125956067334155No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5950.20092187684670842No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5770.19484356796731223No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5600.1891029429145491No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5550.18741452378138349No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5480.18505073699495161No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5430.183362317861786No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5310.17931011194218852No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG5280.17829706046228916No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5080.1715433839296267No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5050.17053033244972732No Hit
ATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCT5020.16951728096982796RNA PCR Primer, Index 40 (95% over 22bp)
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4860.16411433974369796No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4700.158711398517568No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4520.15263308963817177No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4510.15229540581153866No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG4510.15229540581153866No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4310.14554172927887618No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4250.14351562631907744No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4040.13642426595978185No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3980.1343981629999831No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3810.12865753794722004No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3730.12595606733415504No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3720.1256183835075219No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3670.1239299643743563No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3600.12156617758792443No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3580.12089080993465819No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG3540.1195400746281257No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3440.11616323636179444No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3440.11616323636179444No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3360.11346176574872947No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG3350.11312408192209633No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC3340.11278639809546322No Hit
CTTTACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA3340.11278639809546322No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3290.11109797896229759No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3260.11008492748239823No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT3230.10907187600249886No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA3220.10873419217586573No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3220.10873419217586573No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG3180.10738345686933325No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3100.10468198625626826No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC3070.10366893477636889No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG2990.10096746416330389No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2970.10029209651003766No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACAC200.001839730437.03
TAGCAAC251.231413E-437.01
GATGGCT200.001839730437.09
TCAGAGT200.001839730437.03
ACTCAGA200.001839730437.01
CTTATAC24750.035.505051
CGTCTTC6100.034.8770537
TTATACA26800.032.789182
ATTACTT401.5947608E-632.3752
GATTACG401.5947608E-632.3751
TCGCCGA303.5930844E-430.8333321
TTTACAC303.5930844E-430.8333322
CAGACCA303.5930844E-430.8333324
TATACAC28800.030.7690963
GACAGAC551.8459104E-830.27272816
AGACAGA551.8459104E-830.27272815
ACAGACA551.8459104E-830.27272817
CAGCTAG250.005489836429.69
ATTGCTC250.005489836429.629
AGCTAGG250.005489836429.610
Produced by FastQC (version 0.11.5)