##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631027.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	296135
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21618180897226	31.0	31.0	34.0	28.0	34.0
2	31.264470596180796	31.0	31.0	34.0	28.0	34.0
3	30.695969743529133	31.0	31.0	34.0	26.0	34.0
4	34.82942576865281	37.0	35.0	37.0	32.0	37.0
5	35.06753338848836	35.0	35.0	37.0	32.0	37.0
6	35.27092711094602	37.0	35.0	37.0	32.0	37.0
7	35.270562412413256	37.0	35.0	37.0	32.0	37.0
8	35.29410910564439	37.0	35.0	37.0	32.0	37.0
9	37.108636263866146	39.0	37.0	39.0	33.0	39.0
10	36.93250375673257	39.0	37.0	39.0	33.0	39.0
11	36.9617370456042	39.0	37.0	39.0	33.0	39.0
12	36.836287504009995	39.0	37.0	39.0	32.0	39.0
13	36.954351900315736	39.0	37.0	39.0	33.0	39.0
14	37.76239552906613	39.0	37.0	41.0	32.0	41.0
15	37.77436642072028	39.0	37.0	41.0	32.0	41.0
16	37.77196886555119	39.0	37.0	41.0	32.0	41.0
17	37.7714859776791	39.0	37.0	41.0	32.0	41.0
18	37.91451871612609	40.0	37.0	41.0	33.0	41.0
19	37.82118628328296	40.0	37.0	41.0	32.0	41.0
20	37.947696152092796	40.0	37.0	41.0	33.0	41.0
21	37.848633224711705	40.0	37.0	41.0	33.0	41.0
22	37.649774596045724	39.0	37.0	41.0	32.0	41.0
23	37.757840174244855	39.0	37.0	41.0	33.0	41.0
24	37.50359126749624	39.0	36.0	41.0	32.0	41.0
25	37.488125348236444	39.0	36.0	41.0	32.0	41.0
26	37.270390193661676	39.0	36.0	41.0	31.0	41.0
27	37.08749387948064	39.0	36.0	40.0	31.0	41.0
28	37.267280125618385	39.0	36.0	41.0	31.0	41.0
29	37.08096645111183	39.0	36.0	40.0	31.0	41.0
30	36.98465902375606	39.0	35.0	40.0	31.0	41.0
31	36.85490063653401	39.0	35.0	40.0	31.0	41.0
32	36.566572678001585	38.0	35.0	40.0	30.0	41.0
33	36.438587130869365	38.0	35.0	40.0	30.0	41.0
34	36.225572796190924	38.0	35.0	40.0	30.0	41.0
35	36.227203809073565	38.0	35.0	40.0	30.0	41.0
36	36.15395005656204	38.0	35.0	40.0	30.0	41.0
37	35.97759467810289	38.0	35.0	40.0	30.0	41.0
38	35.930423624360515	38.0	35.0	40.0	30.0	41.0
39	35.76019045367822	38.0	35.0	40.0	29.0	41.0
40	35.38013406047917	38.0	34.0	40.0	26.0	41.0
41	35.31850338528036	38.0	34.0	40.0	26.0	41.0
42	35.108778091073326	38.0	34.0	40.0	26.0	41.0
43	34.08775051918888	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	6.0
10	16.0
11	25.0
12	11.0
13	21.0
14	14.0
15	13.0
16	11.0
17	13.0
18	24.0
19	35.0
20	70.0
21	133.0
22	248.0
23	468.0
24	766.0
25	1241.0
26	1911.0
27	3014.0
28	4231.0
29	5684.0
30	7147.0
31	9301.0
32	11607.0
33	14459.0
34	18443.0
35	24278.0
36	31867.0
37	45304.0
38	63015.0
39	52757.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.90323669947828	23.442686612524692	17.159065966535533	26.495010721461497
2	11.576139260810104	20.83576747091698	44.22172320056731	23.366370067705606
3	13.267259864588784	31.493069039458355	37.109426444020464	18.130244651932394
4	8.941192361591842	21.79478953855505	27.989599338139698	41.274418761713406
5	12.872507471254666	37.29143802657572	31.002414439360425	18.83364006280919
6	24.716767690411466	37.95971431948267	15.444982862545798	21.878535127560063
7	20.57304945379641	34.49035068465396	15.431475509480475	29.505124352069156
8	26.949870835936313	29.36802471845611	24.595876880476812	19.086227565130766
9	19.927397977273877	11.259729515254866	14.399513735289649	54.41335877218161
10	15.43451466392017	27.592145474192513	27.100815506441318	29.872524355445996
11	29.23599034224256	19.473888598105592	23.4335691492056	27.85655191044625
12	18.995052931939824	26.580444729599677	20.729059381700914	33.695442956759585
13	35.78064058621913	15.891063197528155	24.65024397656474	23.67805223968798
14	22.79467134921573	24.978134972225504	21.763047258851536	30.464146419707227
15	28.75276478633056	24.170395258919076	18.02826413628919	29.048575818461174
16	29.97585560639573	21.474327587080218	17.242811555540545	31.307005250983504
17	18.214327924764042	34.824657673020745	18.18258564506053	28.77842875715468
18	27.776520843534197	17.663227919698787	21.243689533489793	33.31656170327722
19	23.87458422678846	29.60372802944603	22.861195063062453	23.660492680703058
20	19.988856433721107	18.879227379404664	26.412278183936383	34.71963800293785
21	28.359700812129603	19.123710469887044	23.337329258615156	29.179259459368197
22	17.380924240633494	35.23224205176693	27.93016698465227	19.456666722947304
23	24.28351934084117	19.57755753288196	22.21655663801982	33.92236648825705
24	26.58415925169264	31.64401370996336	24.199436068009522	17.572390970334475
25	24.544886622655206	26.151248585948977	25.136171003089807	24.167693788306007
26	30.351360021611768	27.85992874871258	21.78195755314299	20.00675367653266
27	19.442484002228714	30.145035203538928	33.723808398196766	16.688672396035592
28	17.24416229084708	19.959815624630657	27.030239586675	35.76578249784726
29	18.274435645904738	29.959646782717343	29.27583703378527	22.490080537592654
30	20.043561213635673	17.99348270214598	37.01487497256319	24.948081111655156
31	24.513144342951694	22.799398922788594	21.53207152143448	31.15538521282523
32	26.414641970722812	20.668613976733585	33.84098468603846	19.07575936650514
33	23.435257568338763	19.48300606142469	30.625896972664496	26.45583939757205
34	17.736842993904805	36.29898526010097	24.204163641582387	21.760008104411842
35	26.670268627484084	19.20542995593226	37.477501815050566	16.646799601533086
36	30.469887044759993	19.742347240278928	22.25640332956253	27.531362385398552
37	21.21971398179884	25.785874685531933	29.63175578705658	23.362655545612643
38	20.0530163607814	17.906022591048	31.552163709119153	30.488797339051448
39	28.65652489574012	19.303358265655866	25.0932851571074	26.946831681496615
40	17.776352001620882	18.48650108903034	42.51338072163034	21.22376618771844
41	23.946173198034682	21.973086599017343	23.143498742127747	30.937241460820236
42	24.525976328363754	17.997197224238946	35.9791986762794	21.4976277711179
43	24.694142874027047	18.159960828676112	33.24227126141793	23.903625035878907
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	6.5
2	12.0
3	70.5
4	129.0
5	129.0
6	205.5
7	282.0
8	333.0
9	384.0
10	554.5
11	725.0
12	725.0
13	1147.5
14	1570.0
15	4012.0
16	6454.0
17	6610.0
18	6766.0
19	6766.0
20	5454.0
21	4142.0
22	3174.5
23	2207.0
24	2162.0
25	2117.0
26	2117.0
27	2116.5
28	2116.0
29	2065.5
30	2015.0
31	2050.0
32	2085.0
33	2085.0
34	2423.5
35	2762.0
36	2800.5
37	2839.0
38	3217.0
39	3595.0
40	3595.0
41	4799.0
42	6003.0
43	7833.0
44	9663.0
45	12925.5
46	16188.0
47	16188.0
48	22380.0
49	28572.0
50	46457.5
51	64343.0
52	45715.5
53	27088.0
54	27088.0
55	37651.0
56	48214.0
57	39552.0
58	30890.0
59	20588.0
60	10286.0
61	10286.0
62	8419.0
63	6552.0
64	5185.0
65	3818.0
66	3084.0
67	2350.0
68	2350.0
69	1828.5
70	1307.0
71	901.0
72	495.0
73	291.5
74	88.0
75	88.0
76	64.0
77	40.0
78	31.5
79	23.0
80	17.0
81	11.0
82	11.0
83	6.5
84	2.0
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	296135.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.53727185236463
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.9188585512381	12.437233018724568
2	11.110255324065161	3.8968713593462443
3	4.586590672776986	2.4130886251203
4	2.684176069626834	1.8829250173062961
5	1.7868833519467016	1.5668529555776924
6	1.1899718873955405	1.2521316291556217
7	0.9011437593869142	1.1062522160501123
8	0.7336234451419109	1.0292603035777603
9	0.5603265683367351	0.8843939419521502
>10	4.449878692186236	15.718844445945262
>50	0.5930604228443794	7.284177824303105
>100	0.41398698347903107	13.57691593361136
>500	0.03851041706781684	4.458101879210495
>1k	0.02310625024069011	9.383558174481234
>5k	0.0038510417067816843	4.998396001823493
>10k+	0.005776562560172527	18.11099667381431
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT	21330	7.202796022084522	No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG	18115	6.117142519459031	No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT	14188	4.791058132270755	TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC	9576	3.23366032383879	No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC	5226	1.764735677984703	RNA PCR Primer, Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC	4861	1.6414810812636131	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4350	1.4689246458540868	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4261	1.4388707852837388	No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG	2301	0.777010485082817	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2192	0.7402029479798065	No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA	1834	0.6193121380451483	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1658	0.5598797845577186	No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT	1435	0.48457629121853213	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1366	0.46127610718084655	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1213	0.4096104817059787	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1162	0.39238860654768937	No Hit
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT	1155	0.3900248197612575	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	990	0.3343069883667922	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	981	0.3312678339270941	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	937	0.31640974555523665	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	856	0.28905735559795365	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	831	0.28061525993212555	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	822	0.27757610549242745	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	620	0.20936397251253652	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	617	0.2083509210326371	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	596	0.20125956067334155	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	595	0.20092187684670842	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	577	0.19484356796731223	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	560	0.1891029429145491	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	555	0.18741452378138349	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	548	0.18505073699495161	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	543	0.183362317861786	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	531	0.17931011194218852	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	528	0.17829706046228916	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	508	0.1715433839296267	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	505	0.17053033244972732	No Hit
ATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCT	502	0.16951728096982796	RNA PCR Primer, Index 40 (95% over 22bp)
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	486	0.16411433974369796	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	470	0.158711398517568	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	452	0.15263308963817177	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	451	0.15229540581153866	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	451	0.15229540581153866	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	431	0.14554172927887618	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	425	0.14351562631907744	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	404	0.13642426595978185	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	398	0.1343981629999831	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	381	0.12865753794722004	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	373	0.12595606733415504	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	372	0.1256183835075219	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	367	0.1239299643743563	No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG	360	0.12156617758792443	No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA	358	0.12089080993465819	No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG	354	0.1195400746281257	No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	344	0.11616323636179444	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	344	0.11616323636179444	No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA	336	0.11346176574872947	No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG	335	0.11312408192209633	No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC	334	0.11278639809546322	No Hit
CTTTACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA	334	0.11278639809546322	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	329	0.11109797896229759	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	326	0.11008492748239823	No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT	323	0.10907187600249886	No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA	322	0.10873419217586573	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	322	0.10873419217586573	No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG	318	0.10738345686933325	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	310	0.10468198625626826	No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	307	0.10366893477636889	No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG	299	0.10096746416330389	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	297	0.10029209651003766	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	3.376838266331234E-4	0.0
9	0.0	0.0	0.0	3.376838266331234E-4	0.0
10	0.0	0.0	0.0	3.376838266331234E-4	0.0
11	0.0	0.0	0.0	3.376838266331234E-4	0.0
12	0.0	0.0	0.0	3.376838266331234E-4	0.0
13	0.0	0.0	0.0	6.753676532662468E-4	0.0
14	0.0	0.0	0.0	6.753676532662468E-4	0.0
15	0.0	0.0	0.0	0.0010130514798993703	0.0
16	0.0	0.0	0.0	0.0010130514798993703	0.0
17	0.0	0.0	0.0	0.0010130514798993703	0.0
18	0.0	0.0	0.0	0.0010130514798993703	0.0
19	0.0	0.0	0.0	0.0010130514798993703	0.0
20	0.0	0.0	0.0	0.0013507353065324937	0.0
21	0.0	0.0	0.0	0.0030391544396981105	0.0
22	0.0	0.0	0.0	0.003376838266331234	0.0
23	0.0	0.0	0.0	0.004389889746230604	0.0
24	0.0	0.0	0.0	0.006753676532662468	0.0
25	0.0	0.0	0.0	0.007091360359295591	0.0
26	0.0	0.0	0.0	0.008779779492461209	0.0
27	0.0	0.0	0.0	0.012494301585425567	0.0
28	0.0	0.0	0.0	0.029040809090448615	0.0
29	0.0	0.0	0.0	0.07091360359295591	0.0
30	0.0	0.0	0.0	0.1168386040150607	0.0
31	0.0	0.0	0.0	0.17694632515575667	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACAC	20	0.0018397304	37.0	3
TAGCAAC	25	1.231413E-4	37.0	1
GATGGCT	20	0.0018397304	37.0	9
TCAGAGT	20	0.0018397304	37.0	3
ACTCAGA	20	0.0018397304	37.0	1
CTTATAC	2475	0.0	35.50505	1
CGTCTTC	610	0.0	34.87705	37
TTATACA	2680	0.0	32.78918	2
ATTACTT	40	1.5947608E-6	32.375	2
GATTACG	40	1.5947608E-6	32.375	1
TCGCCGA	30	3.5930844E-4	30.833332	1
TTTACAC	30	3.5930844E-4	30.833332	2
CAGACCA	30	3.5930844E-4	30.833332	4
TATACAC	2880	0.0	30.769096	3
GACAGAC	55	1.8459104E-8	30.272728	16
AGACAGA	55	1.8459104E-8	30.272728	15
ACAGACA	55	1.8459104E-8	30.272728	17
CAGCTAG	25	0.0054898364	29.6	9
ATTGCTC	25	0.0054898364	29.6	29
AGCTAGG	25	0.0054898364	29.6	10
>>END_MODULE